Spherical nucleic acids: A whole new ball game

Williams, Sharon

doi:10.1073/pnas.1313483110

Cited by 17 publications

(8 citation statements)

References 7 publications

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“…Conjugation of biomolecules (e.g., DNA, proteins and peptides) to inorganic nanoparticles (NPs) opened up a broad range of exciting research in photonics, , biocatalysis, ,− bioelectronics, ,, biosensors, − drug delivery, , and gene regulation. − NPs used in drug and gene delivery are particularly attractive, since their small size allows for tissue penetration as well as elimination, while still affording a high number of targeting moieties and bioactive molecules on NP surfaces . NPs in these studies are often conjugated with molecules that have high affinity to specific biomolecules for the purpose of targeting diseased cells or tissues; however such molecules tend to also exhibit nonspecific affinity to other biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle Assembly and Gelatin Binding Mediated by Triple Helical Collagen Mimetic Peptide

San

Tarbet

et al. 2016

ACS Appl. Mater. Interfaces

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Peptide-conjugated nanoparticles (NPs) have promising potential for applications in biosensing, diagnosis, and therapeutics because of their appropriate size, unique self-assembly, and specific substrate-binding properties. However, controlled assembly and selective target binding are difficult to achieve with simple peptides on NP surfaces because high surface energy makes NPs prone to self-aggregate and adhere nonspecifically. Here, we report the self-assembly and gelatin binding properties of collagen mimetic peptide (CMP) conjugated gold NPs (CMP-NPs). We show that the orientation of CMPs displayed on the NP surface can control NP assembly either by promoting or hindering triple helical folding between CMPs of neighboring NPs. We also show that CMP-NPs can specifically bind to denatured collagen by forming triple-helical hybrids between denatured collagen strands and CMPs, demonstrating their potential use for detection and selective removal of gelatin from protein mixtures. CMP conjugated NPs offer a simple and effective method for NP assembly and for targeting denatured collagens with high specificity. Therefore, they may lead to new types of functional nanomaterials for detection and study of denatured collagen associated with diseases characterized by high levels of collagen degradation.

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Section: Introductionmentioning

confidence: 99%

Nanoparticle Assembly and Gelatin Binding Mediated by Triple Helical Collagen Mimetic Peptide

San

Tarbet

et al. 2016

ACS Appl. Mater. Interfaces

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“…[1][2][3] This elegant natural mechanism has inspired the construction of enzyme complexes by assembling enzymes on scaffolds, which demonstrated potential applications in cell imaging, RNA interference and intracellular sensing. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] Like the invention of spherical nucleic acids (SNAs), 15,16,[20][21][22][23][24][25][26][27][28][29][30][31] the construction of enzyme complexes by assembling DNAzymes on the surface of nanoparticles could facilitate the enzyme complexes to penetrate the cell membrane, cross the epidermal barrier and the blood-brain barrier and avoid attack by the immune system, which have been identified by accumulated studies of SNA.…”

Section: Introductionmentioning

confidence: 99%

Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes

et al. 2015

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Enzyme complexes are assembled at the two-dimensional lipid membrane or prearranged on three-dimensional scaffolding proteins to regulate their catalytic activity in cells. Inspired by nature, we have developed gold nanoparticle-based spherical DNAzymes (SNAzymes) with programmably engineered activities by exploiting poly-adenine (polyA)-Au interactions. In a SNAzyme, AuNPs serve as the metal core, which is decorated with a functional shell of DNAzymes. Conventional thiolated DNAzyme-based assembly leads to disordered structures with suppressed activity. In contrast, by using an anchoring block of polyA tails, we find that the activity of SNAzymes can be programmably regulated. By using a polyA30 tail, SNAzymes demonstrated remarkably enhanced binding affinity compared to the thiolated DNAzyme-based assembly (∼75-fold) or individual DNAzymes in the solution phase (∼10-fold). More significantly, this increased affinity is directly translated to the sensitivity improvement in the SNAzyme-based lead sensor. Hence, this design of SNAzymes may provide new opportunities for developing biosensors and bioimaging probes for theranostic applications.

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“…First of all, the affinity of SNA to complementary nucleic acids is higher than that of linear counterpart due to its special geometry, thereby increasing the stability of the structure (Seferos et al, 2009). Second, SNA can enter a variety of cells and with excellent cellular uptake in the absence of an auxiliary transfection agent (Williams, 2013). Finally, SNAs is composed of biologically compatible materials and are not toxic to cells (Melamed et al, 2018), making SNA a powerful tool in numerous biomedical applications.…”

Section: Spherical Nucleic Acidsmentioning

confidence: 99%

DNA Nanostructure as an Efficient Drug Delivery Platform for Immunotherapy

Chi

Yang

et al. 2020

Front. Pharmacol.

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Immunotherapy has received increasing attention due to its low potential side effects and high specificity. For instance, cancer immunotherapy has achieved great success. CpG is a well-known and commonly used immunotherapeutic and vaccine adjuvant, but it has the disadvantage of being unstable and low in efficacy and needs to be transported through an effective nanocarrier. With perfect structural programmability, permeability, and biocompatibility, DNA nanostructures are one of the most promising candidates to deliver immune components to realize immunotherapy. However, the instability and low capability of the payload of ordinary DNA assemblies limit the relevant applications. Consequently, DNA nanostructure with a firm structure, high drug payloads is highly desirable. In the paper, the latest progress of biostable, high-payload DNA nanoassemblies of various structures, including cage-like DNA nanostructure, DNA particles, DNA polypods, and DNA hydrogel, are reviewed. Cage-like DNA structures hold drug molecules firmly inside the structure and leave a large space within the cavity. These DNA nanostructures use their unique structure to carry abundant CpG, and their biocompatibility and size advantages to enter immune cells to achieve immunotherapy for various diseases. Part of the DNA nanostructures can also achieve more effective treatment in conjunction with other functional components such as aPD1, RNA, TLR ligands.

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Spherical nucleic acids: A whole new ball game

Cited by 17 publications

References 7 publications

Nanoparticle Assembly and Gelatin Binding Mediated by Triple Helical Collagen Mimetic Peptide

Nanoparticle Assembly and Gelatin Binding Mediated by Triple Helical Collagen Mimetic Peptide

Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes

DNA Nanostructure as an Efficient Drug Delivery Platform for Immunotherapy

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