Spherical bioactive glass particles and their interaction with human mesenchymal stem cells in vitro

Labbaf, Sheyda; Tsigkou, Olga; Müller, Karin H.; Stevens, Molly M.; Porter, Alexandra E.; Jones, Julian R.

doi:10.1016/j.biomaterials.2010.08.082

Cited by 176 publications

(149 citation statements)

References 46 publications

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“…The particles were then heated to 680˚C for 3 h, at a heating rate of 3˚/min. 680˚C was chosen to allow for the removal of nitrates and incorporation of calcium into the network, without crystallisation [5,23]. Subsequently the particles were washed 3 times in ethanol to remove any excess calcium not incorporated into the network.…”

Section: Particle Synthesismentioning

confidence: 99%

“…Labbaf et al incorporated calcium by adapting the process used by Zhao et al [29] in which Boltorn™ polymer was used as a template in the sol-gel process before being burnt out during calcination. The submicron particles were shown to be of composition 86 mol% SiO2 and 14 mol% CaO, verified by quantitative inductively coupled plasma optical emission spectroscopy (ICP) analysis [23]. However, despite being spherical and dispersed, the resulting particles did not show a homogeneous size distribution even after optimisation of the polymer:TEOS ratio.…”

Section: Introductionmentioning

confidence: 95%

“…Bioactive glass nanoparticles can also be incorporated into nanocomposites [22]. Whilst the synthesis of monodispersed Stӧber silica nanoparticles has been achieved previously in literature, the addition of calcium significantly complicates the procedure and can cause the particles to become irregular in morphology [23] or to agglomerate [24][25][26]. While nominal compositions of particles are quoted in the literature, the final compositions are rarely ratified by analytical techniques or only non-quantitative energy-dispersive X-ray spectroscopy (EDX) data is provided [3,25,27].…”

Section: Introductionmentioning

confidence: 99%

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Controlling particle size in the Stöber process and incorporation of calcium

Greasley

Page

Sirovica

et al. 2016

Journal of Colloid and Interface Science

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The Stӧber process is commonly used for synthesising spherical silica particles. This article reports the first comprehensive study of how the process variables can be used to obtain monodispersed particles of specific size. The modal particle size could be selected within in the range 20 -500 nm. There is great therapeutic potential for bioactive glass nanoparticles, as they can be internalised within cells and perform sustained delivery of active ions. Biodegradable bioactive glass nanoparticles are also used in nanocomposites. Modification of the Stӧber process so that the particles can contain cations such as calcium, while maintaining monodispersity, is desirable. Here, while calcium incorporation is achieved, with a homogenous distribution, careful characterisation shows that much of the calcium is not incorporated. A maximum of 10 mol% CaO can be achieved and previous reports are likely to have overestimated the amount of calcium incorporated.

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Section: Particle Synthesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Controlling particle size in the Stöber process and incorporation of calcium

Greasley

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Sirovica

et al. 2016

Journal of Colloid and Interface Science

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136

151

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“…Nanoparticulate bioactive glasses with a chemical structure that is similar to Hench's 45S5 composition (Hench 1998) also display antibacterial properties and have potential for dentin remineralisation when applied to demineralised dentin matrix (Curtis et al 2010;Vollenweider et al 2007;Waltimo et al 2007). Labbaf et al investigated sub-micron bioactive glass particles of composition 85 mol% SiO 2 and 15 mol% CaO, synthesised by the sol-gel process, confirming the non-toxicity of the sub-micron bioactive glass particles (Labbaf et al 2011). It is thus of high interest to develop and investigate bioactive glass nanoparticles with different chemical composition.…”

Section: Introductionmentioning

confidence: 99%

Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells

et al. 2013

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The present study investigates a new family of bioactive glass nanoparticles with and without Sr-doping focusing on the influence of the nanoparticles on human bone marrow stromal cells (hBMSCs) in vitro. The bioactive glass nanoparticles were fabricated by flame spray synthesis and a particle diameter of 30-35 nm was achieved. Glass nanoparticles were undoped (BG 13-93-0Sr) or doped with 5 wt% strontium (Sr) ) and used at concentrations of 10 and 100 g/cm² (particles per culture plate area), respectively. Cells were cultured for 14 days after which the samples were analysed regarding metabolic activity and expression of various bone-specific genes. Cell growth and morphology indicated the high cytocompatibility of the nanoparticulate bioactive glass. The presence of the nanoparticles enhanced cell growth compared to the plain polystyrene control group. At a concentration of 100 g/cm², Sr-doped particles led to significantly enhanced gene expression of osteocalcin, collagen type 1 and vascular endothelial growth factor. Thus, Sr-doped nanoparticles showing a dose-dependent increase of osteogenic differentiation in hBMSCs are a promising biomaterial for bone regeneration purposes.

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“…This is mainly due to their degradation capability which liberates ions such as Ca, P, Si, Na, Sr etc [9,11,16,36] depending upon their initial chemical composition of the glass. Often, sol-gel derived BG structures have a greater bioactivity than melt-derived ones and this is mainly due to a greater surface area, inherent nanoporosity and higher levels of OH on the surface of glass [37]. However, the main drawback of sol-gel derived BGs is their burst release of ions in the first few hours of immersion in body fluid which causes a sudden increase in pH in the surrounding area that result in cell and tissue death hence BGs are often preconditioned to avoid this occurrence.…”

Section: Figurementioning

confidence: 99%

Long term effects of bioactive glass particulates on dental pulp stem cells in vitro

Gholami¹,

Labbaf²,

Houreh³

et al. 2017

Biomedical Glasses

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Bioactive glasses (BG) are known for their ability to induce bone formation by the action of their dissolution products. Glasses can deliver active ions at a sustained rate, determined by their composition and surface area. Nanoporous sol-gel derived BGs can biodegrade rapidly, which can lead to a detrimental burst release of ions and a pH rise. The addition of phosphate into the glass can buffer the pH during dissolution. Here, dissolution of BG with composition 60 mol% SiO 2 , 28 mol% CaO and 12 mol% P 2 O 5 at 600 µg/ml were investigated. Initially, the dissolution and apatite formation of the BG particulates were examined in simulated body fluid using FTIR and XRD. BG particulates were indirectly exposed to dental pulp stem cells, and the effect of 14 days continuous ion release on human dental pulp stem cells (hDPSC) viability and differentiation was evaluated. Alamar blue assay showed that cell proliferation was not inhibited by the continuous release of Ca, P and soluble silica. In fact, hDPSC in the presence of BG particulate displayed a higher density of mineralized nodules than untreated cells, as assessed by Alizarin red. The results will have a great contribution to the in vivo application of this particular BG.

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Spherical bioactive glass particles and their interaction with human mesenchymal stem cells in vitro

Cited by 176 publications

References 46 publications

Controlling particle size in the Stöber process and incorporation of calcium

Controlling particle size in the Stöber process and incorporation of calcium

Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells

Long term effects of bioactive glass particulates on dental pulp stem cells in vitro

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