Sphere-Derived Multipotent Progenitor Cells Obtained From Human Oral Mucosa Are Enriched in Neural Crest Cells

Abe, Sumiyoshi; Yamaguchi, Satoshi; Sato, Yutaka; Harada, Kiyoshi

doi:10.5966/sctm.2015-0111

Cited by 30 publications

(75 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with our results, Davies et al suggested that NC‐derived progenitors in oral mucosa possess a multipotent capacity 10 . Also, a recent study showed that human oral mucosa‐derived cells were capable of differentiating into NC lineages in vitro and generated ectopic bone tissues in vivo 36 . Since abundant GFP‐positive NCDCs were seen in buccal mucosa, we consider that this type of tissue may be beneficial to harvesting NCDCs for use in regenerative medicine strategies.…”

Section: Distribution and Clinical Potential Of Ncdcs In Oral And Densupporting

confidence: 91%

Prospects of neural crest‐derived cells from oral and dentofacial tissues for application in regenerative medicine

Suzawa

Yoshida

Takahashi

et al. 2020

Oral Science International

View full text Add to dashboard Cite

During vertebrate development, neural crest cells arise in the dorsal region of the fusing neural tube, then migrate extensively across the embryonic body to differentiate into various cell types. In adults, subsets of neural crest‐derived cells (NCDCs) reside as stem cells and are considered useful as cell sources for autologous cell therapy. Previous studies have suggested that these NCDC subsets persist into adulthood in mammals, especially those within dentofacial compartments. We have found NCDCs in a wide variety of tissues, including palate, gingiva, and tongue, as well as hair follicles, submandibular glands, and buccal mucosa in adult mice. NCDCs from buccal mucosa can also form neurosphere‐like structures that have a capability to differentiate into osteoblasts. These findings indicate that NCDCs reside in various adult oral and dentofacial regions, and possess potential to differentiate into osteoblastic cells, thus suggesting that those in adults may be a useful source for bone regeneration strategies. In recent years, several researchers have reported regeneration experiments using NCDCs obtained from oral and dentofacial tissues. Here, we review findings related to the distribution of NCDCs, with focus on the oral and dentofacial regions, as well as the future prospects of NCDC‐based regenerative therapies.

show abstract

Section: Distribution and Clinical Potential Of Ncdcs In Oral And Densupporting

confidence: 91%

Prospects of neural crest‐derived cells from oral and dentofacial tissues for application in regenerative medicine

Suzawa

Yoshida

Takahashi

et al. 2020

Oral Science International

View full text Add to dashboard Cite

show abstract

“…In human, the confirmation of the presence of neural crest cells in adult tissues is less obvious and mainly based on their expression profile, associated with their differentiating capacity and their ability to follow migration pathways when injected in chicken embryos. In those conditions, adult human NCSC have been identified in skin [28–32], hair follicle [30,31], in dental pulp [33–36], periodontal ligament [37,38], in cornea [39], inferior turbinate [40], olfactory or oral mucosa [41,42] and in adipose tissue [43]. …”

Section: Discussionmentioning

confidence: 99%

Human bone marrow harbors cells with neural crest-associated characteristics like human adipose and dermis tissues

et al. 2017

View full text Add to dashboard Cite

Adult neural crest stem-derived cells (NCSC) are of extraordinary high plasticity and promising candidates for use in regenerative medicine. Several locations such as skin, adipose tissue, dental pulp or bone marrow have been described in rodent, as sources of NCSC. However, very little information is available concerning their correspondence in human tissues, and more precisely for human bone marrow. The main objective of this study was therefore to characterize NCSC from adult human bone marrow. In this purpose, we compared human bone marrow stromal cells to human adipose tissue and dermis, already described for containing NCSC. We performed comparative analyses in terms of gene and protein expression as well as functional characterizations. It appeared that human bone marrow, similarly to adipose tissue and dermis, contains NESTIN+ / SOX9+ / TWIST+ / SLUG+ / P75NTR+ / BRN3A+/ MSI1+/ SNAIL1+ cells and were able to differentiate into melanocytes, Schwann cells and neurons. Moreover, when injected into chicken embryos, all those cells were able to migrate and follow endogenous neural crest migration pathways. Altogether, the phenotypic characterization and migration abilities strongly suggest the presence of neural crest-derived cells in human adult bone marrow.

show abstract

“…One of the major challenges facing the field of stem cell-based tissue engineering, including 3D bio-printing of complex tissues and organs, particularly the neural tissues, is the lack of a readily accessible source of stem cells. Previous studies have demonstrated that numerous adult tissues, including most of the oral and craniofacial tissues, originate from ectoderm-derived migratory neural crest cells during development 25 , 26 , while specific subpopulations of adult progenitor cells 27 , particularly those from oral and craniofacial tissues 16 , 28 – 30 , confer neural crest stem-like cells properties. Most recently, we have shown that GMSCs of neural crest-origin could be easily and readily converted into neural progenitor-like cells and exhibited enhanced capability of differentiating into neuronal and Schwann cells both in vitro and in vivo 17 .…”

Section: Discussionmentioning

confidence: 99%

3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration

Zhang

Nguyen

Shi

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Despite the promising neuro-regenerative capacities of stem cells, there is currently no licensed stem cell-based product in the repair and regeneration of peripheral nerve injuries. Here, we explored the potential use of human gingiva-derived mesenchymal stem cells (GMSCs) as the only cellular component in 3D bio-printed scaffold-free neural constructs that were transplantable to bridge facial nerve defects in rats. We showed that GMSCs have the propensity to aggregate into compact 3D-spheroids that could produce their own matrix. When cultured under either 2D- or 3D-collagen scaffolds, GMSC spheroids were found to be more capable of differentiating into both neuronal and Schwann-like cells than their adherent counterparts. Using a scaffold-free 3D bio-printer system, nerve constructs were printed from GMSC spheroids in the absence of exogenous scaffolds and allowed to mature in a bioreactor. In vivo transplantation of the GMSC-laden nerve constructs promoted regeneration and functional recovery when used to bridge segmental defects in rat facial nerves. Our findings suggest that GMSCs represent an easily accessible source of MSCs for 3D bio-printing of scaffold-free nervous tissue constructs with promising potential application for repair and regeneration of peripheral nerve defects.

show abstract

Sphere-Derived Multipotent Progenitor Cells Obtained From Human Oral Mucosa Are Enriched in Neural Crest Cells

Cited by 30 publications

References 52 publications

Prospects of neural crest‐derived cells from oral and dentofacial tissues for application in regenerative medicine

Prospects of neural crest‐derived cells from oral and dentofacial tissues for application in regenerative medicine

Human bone marrow harbors cells with neural crest-associated characteristics like human adipose and dermis tissues

3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration

Contact Info

Product

Resources

About