Sphaeroplast Formation in Yeast During the Transition from Exponential Phase to Stationary Phase

Deutch, Charles E.; Parry, James M.

doi:10.1099/00221287-80-1-259

Cited by 59 publications

(23 citation statements)

References 21 publications

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“…Upon exhaustion of available glucose, ethanol is utilized aerobically, the rate of proliferation and mass increase slows, and cell size at commitment to division decreases (Lord & Wheals, 1980;Lorincz & Carter, 1979). Finally, upon ethanol limitation, cell proliferation ceases, and cells accumulate in the G1 phase of the cell cycle (Hartwell, 1974) and undergo complex physiological changes which include the accumulation of the reserve carbohydrates glycogen and trehalose (Lillie & Pringle, 1980) and result in the cells becoming resistant to environmental stresses such as heat shock (Parry et al, 1976;Saul et al, 1985;Schenberg-Frascino & Moustacchi, 1972), nystatin treatment (Saul et al, 1985;Snow, 1966) and cell wall digestion with zymolyase (Deutch & Parry, 1973;Saul et al, 1985). The concerted changes in metabolic activity, grpwth rate, cell proliferation and cell size that occur through this growth cycle in response to changing environmental conditions show that all these cell functions must be finely integrated with respect to each other and to the environment.…”

Section: Introductionmentioning

confidence: 99%

Regulation of the Saccharomyces cerevisiae WH12 gene

Mountain

Sudbery²

1990

Journal of General Microbiology

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WHZ2 mRNA levels were followed through the growth cycle in WHZ2 mutant and wild-type cells of Saccharomyces cereuisiae. Levels were high during the first (glucose) phase of growth, and were reduced sharply during the second (ethanol) phase of growth. Transcript levels of the glycolytic genes PDCl and PYKl were also measured; they each showed a pattern similar to that of WHZ2, whereas transcript levels of the CDC7 gene remained constant throughout the cycle, showing that a decrease in transcription is not a general feature of genes. These results make it unlikely that the WHZ2 product acts as an inybitor of wll proliferation which is activated upon carbon starvation. No difference was observed between the pattern of expression of mutant and wild-type strains, showing that the mutant phenotype was not the result of a change in regulation at the transcriptional level.

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Section: Introductionmentioning

confidence: 99%

Regulation of the Saccharomyces cerevisiae WH12 gene

Mountain

Sudbery²

1990

Journal of General Microbiology

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“…In many organisms, stationary-phase cells are morphologically and/or physiologically distinct from those in the mitotic cycle (1)(2)(3)(4)(5). The induction of nonproliferative cells into a mitotic cycle is a tightly regulated process and nutrients play a critical role in this regulation (6).…”

mentioning

confidence: 99%

Glucose induces cAMP-independent growth-related changes in stationary-phase cells of Saccharomyces cerevisiae.

Granot

Snyder

1991

Proc. Natl. Acad. Sci. U.S.A.

123

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Nutrients play a critical role in the decision to initiate a new cell cycle. Addition of nutrients to arrested cells such as stationary-phase cells and spores induces them to begin growth. We have analyzed the nutrients required to induce early cellular events in yeast. When stationary-phase cells or spores are incubated in the presence of only glucose, morphological and physiological changes characteristic of mitotically growing cells are induced and, in the absence of additional nutrients to support growth, the cells rapidly lose viability. Preincubation of stationary-phase cells in the presence of glucose decreases the time required to reach bud emergence upon the subsequent addition of rich medium. These processes are specifically induced by D-glucose and not by other components such as nitrogen source or L-glucose. The glucoseinduced events are independent of the adenylate cyclase pathway, since strains with a temperature-sensitive mutation in either the adenylate cyclase gene (CDC35) or its regulator (CDC25) undergo glucose-induced cellular changes when incubated at the restrictive temperature. We suggest that glucose triggers events in the induction of a new mitotic cell cycle and that these events are either prior to the adenylate cyclase pathway or are in an alternative pathway.

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“…Two changes that have been well documented are strengthening of the cell wall and accumulation of trehalose (8,9,47,50). In general, different phases of the growth cycle are marked by major alterations in gene expression.…”

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confidence: 99%

Identification and characterization of a novel yeast gene: the YGP1 gene product is a highly glycosylated secreted protein that is synthesized in response to nutrient limitation.

Destruelle¹,

Holzer²,

Klionsky³

1994

Mol. Cell. Biol.

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Nutrient starvation in the yeast Saccharomyces cerevisiae leads to a number of physiological changes that accompany entry into stationary phase. The expression of genes whose products play a role in stress adaptation is regulated in a manner that allows the cell to sense and respond to changing environmental conditions. We have identified a novel yeast gene, YGP1, that displays homology to the sporulation-specific SPS100 gene. The expression of YGP1 is regulated by nutrient availability. The gene is expressed at a basal level during "respiro-fermentative" (logarithmic) growth. When the glucose concentration in the medium falls below 1%, the YGP1 gene is derepressed and the gene product, gp37, is synthesized at levels up to 50-fold above the basal level. The glucose-sensing mechanism is independent of the SNFJ pathway and does not operate when cells are directly shifted to a low glucose concentration. The expression of YGPJ also responds to the depletion of nitrogen and phosphate, indicating a general response to nutrient deprivation. These results suggest that the YGP1 gene product may be involved in cellular adaptations prior to stationary phase and may be a useful marker protein for monitoring early events associated with the stress response.

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Sphaeroplast Formation in Yeast During the Transition from Exponential Phase to Stationary Phase

Cited by 59 publications

References 21 publications

Regulation of the Saccharomyces cerevisiae WH12 gene

Regulation of the Saccharomyces cerevisiae WH12 gene

Glucose induces cAMP-independent growth-related changes in stationary-phase cells of Saccharomyces cerevisiae.

Identification and characterization of a novel yeast gene: the YGP1 gene product is a highly glycosylated secreted protein that is synthesized in response to nutrient limitation.

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