Sphaerisporangium flaviroseum sp. nov. and Sphaerisporangium album sp. nov., isolated from forest soil in China

Cao, Yan-Ru; Jiang, Yi; Xu, Li-Hua; Jiang, Cheng‐Lin

doi:10.1099/ijs.0.006130-0

Cited by 29 publications

(16 citation statements)

References 25 publications

(22 reference statements)

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“…Small amounts of phosphatidylinositol and phosphoglycolipid were also detected. Similar observations of phosphatidylinositol and phosphoglycolipid were reported by Cao et al 2 for S. album and S. flaviroseum. The cellular fatty acid profile was represented by the predominance of iso-C 16: 0 (47.9%).…”

Section: Methodssupporting

confidence: 77%

“…This genus was emended by Cao et al 2 for the strain containing MK-9(H 6 ), MK-9(H 4 ), MK-9(H 2 ) and MK-9 as predominant menaquinones and the DNA G+C contents of 67-72 mol%. The genus formed a phyletic line within the 16S rRNA genes tree of the Streptosporangiaceae, the family of which included the following genera: Acrocarpospora, Herbidospora, Microbispora, Microtetraspora, Nonomuraea, Planobispora, Planomonospora, Planotetraspora, Sphaerisporangium, Streptosporangium and Thermopolyspora.…”

Section: Introductionmentioning

confidence: 99%

“…Sphaerisporangium album and S. flaviroseum were isolated from forest soil samples in China. 2 Sphaerisporangium cinnabarinum, S. melleum and S. rubeum were isolated from sandy soil samples in Bangladesh. 1 Sphaerisporangium viridialbum was transferred from Streptosporangium viridialbum.…”

Section: Introductionmentioning

confidence: 99%

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Sphaerisporangium siamense sp. nov., an actinomycete isolated from rubber-tree rhizospheric soil

et al. 2011

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A Gram-positive aerobic actinomycete, designated SR14.14 T , isolated from the rhizospheric soil of rubber tree was determined taxonomically using a polyphasic approach. The organism contained meso-diaminopimelic acid and the N-acetyl type of peptidoglycan. The predominant menaquinones were MK-9, MK-9(H 2 ) and MK-9(H 4 ). Madurose was detected in the whole-cell hydrolysates. Mycolic acids were not presented. Major phospholipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol mannoside. Major cellular fatty acid was iso-C 16: 0 and the G+C content was 71.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequence suggested that the isolate belongs to the genus Sphaerisporangium. The sequence similarity value between the strain SR14.14 T and its closely related species, Sphaerisporangium album, was 97.8%. DNA-DNA hybridization values between them were well below 70%. Based on genotypic and phenotypic data, strain SR14.14 T represents a novel species in the genus Sphaerisporangium, for which the name Sphaerisporangium siamense sp. nov. is proposed. The type strain is SR14.14 T (¼BCC 41491 T ¼NRRL B-24805 T ¼NBRC 107570 T ). The Journal of Antibiotics (2011) 64, 293-296; doi:10.1038/ja.2011 published online 16 March 2011 Keywords: actinomycete; rhizosphere soil; rubber-tree; Sphaerisporangium INTRODUCTIONThe genus Sphaerisporangium was proposed by Ara and Kudo 1 with Sphaerisporangium melleum as type species. This genus was emended by Cao et al. 2 for the strain containing MK-9(H 6 ), MK-9(H 4 ), MK-9(H 2 ) and MK-9 as predominant menaquinones and the DNA G+C contents of 67-72 mol%. The genus formed a phyletic line within the 16S rRNA genes tree of the Streptosporangiaceae, the family of which included the following genera: Acrocarpospora, Herbidospora, Microbispora, Microtetraspora, Nonomuraea, Planobispora, Planomonospora, Planotetraspora, Sphaerisporangium, Streptosporangium and Thermopolyspora. 1,3,4 At the time of writing, the genus Sphaerisporangium encompassed six species. Sphaerisporangium album and S. flaviroseum were isolated from forest soil samples in China. 2 Sphaerisporangium cinnabarinum, S. melleum and S. rubeum were isolated from sandy soil samples in Bangladesh. 1 Sphaerisporangium viridialbum was transferred from Streptosporangium viridialbum. 1,5 During our investigation of actinomycetes from rhizospheric soil of rubber tree collected from Phatthalung province, Thailand, the strain SR14.14 T was isolated. A polyphasic study was designed to establish the taxonomic status of this strain. Resultant data based on this polyphasic study showed that this isolate represented a novel species of the genus Sphaerisporangium. MATERIALS AND METHODSStrain SR14.14 T was isolated from the rhizospheric soil of rubber tree using the spread plate method on starch casein agar, 6 supplemented with ketokonazole (100 mg ml À1 ) and nalidixic acid (25 mg ml À1 ). The plates were incubated at 28 1C for 2 weeks. The strain was purified and maintained on glucose yeast extract (GYE) agar...

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Section: Methodssupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

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Sphaerisporangium siamense sp. nov., an actinomycete isolated from rubber-tree rhizospheric soil

et al. 2011

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“…Data were taken from this and previous studies (Greiner-Mai et al, 1987;Goodfellow et al, 1990;Kudo et al, 1993;Tamura et al, 2000;Stackebrandt et al, 2001;Tamura & Sakane, 2004;Ara & Kudo, 2007;Goodfellow et al, 2005;Cao et al, 2009). For all taxa, the fatty acid type [saturated fatty acids, unsaturated fatty acids, iso-fatty acids, variable and methyl-branched fatty acids (Kroppenstedt, 1985)] was 3c.…”

Section: Strain 6014mentioning

confidence: 99%

Sinosporangium album gen. nov., sp. nov., a new member of the suborder Streptosporangineae

Zhang

Liu

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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, was isolated from a soil sample collected from Qinghai province, north-west China, and subjected to a polyphasic taxonomic study. The isolate formed elementary branching hyphae and abundant aerial mycelia with globose sporangia on ISP 4 and R2A media. Whole-cell hydrolysates of strain 6014 T contained arabinose, galactose and ribose as diagnostic sugars and meso-diaminopimelic acid as the diagnostic diamino acid. The polar lipids consisted of phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, N-acetylglucosamine-containing phospholipids, two unknown phospholipids and an unknown glycolipid. The menaquinone system contained MK-9(H 2 ) and MK-9(H 4 ). The major fatty acids were C 14 : 0 , i-C 15 : 0 , C 16 : 0 and 10-methyl-C 16 : 1 . The genomic DNA G+C content of the isolate was 69.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 6014 T fell within the radius of the suborder Streptosporangineae, in which the strain formed a distinct lineage next to genera of the family Streptosporangiaceae. Based on data from this polyphasic study, strain 6014 T can be readily distinguished from previously described organisms and represents a member of a novel species within a new genus in the suborder Streptosporangineae. The name Sinosporangium album gen. nov., sp. nov. is proposed with 6014 T (5DSM 45181 T 5KCTC 19655 T ) as the type strain.The suborder Streptosporangineae was proposed by Stackebrandt et al. (1997) based on phylogenetic and signature nucleotide analysis. At the time of writing, there were only three families in this suborder, namely, Streptosporangiaceae, Nocardiopsaceae and Thermomonosporaceae, with Streptosporangiaceae as the type family. In this paper, characterization of strain 6014 T is reported, with proposals for Sinosporangium gen. nov. and Sinosporangium album sp. nov. in the suborder Streptosporangineae (Stackebrandt et al., 1997). Strain 6014T was isolated on Czapek agar (Waksman, 1961) incubated at 28 u C for 3 weeks. The purified strain was maintained on ISP 4 agar and R2A slants at 4 u C and as glycerol suspensions (20 %, v/v) at 220 u C. Biomass for molecular systematic and chemotaxonomic studies was obtained by cultivation in shake flasks using tryptic soy broth (Difco) at 28 u C for 10 days. Cultural characteristics of the isolate were determined after growth for 7-28 days at 28 u C on R2A, ISP 2, ISP 3, ISP 4 and ISP 5 (Shirling & Gottlieb, 1966), Czapek agar, nutrient agar (Difco) and potato agar (Waksman, 1961) media. The coverslip technique (Zhou et al., 1998) was employed to observe characteristics of the hyphae, sporangia and spores. The sporangia and spore chain morphologies were recorded by examining gold-coated dehydrated specimens of 14 day cultures from R2A and ISP 4 agar by scanning electron microscopy (Quanta; FEI).Growth was tested at 0, 4, 10, 15, 20, 28-37 (at intervals of 1 uC), 40, 45 and 55 uC on R2A. Other physiological and biochemical tests were performed at 28 u C. The pH range was examined at ...

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“…The genus Sphaerisporangium which belongs to the family Streptosporangiaceae was established by Ara and Kudo (2007) and emended by Cao et al (2009) …”

Section: Introductionmentioning

confidence: 99%

Sphaerisporangium corydalis sp. nov., isolated from the root of Corydalis yanhusuo L.

Wang

Liu

Cheng

et al. 2015

Antonie van Leeuwenhoek

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Two Gram-stain positive, aerobic actinomycete strains, designated NEAU-YHS12 and NEAU-YHS15(T), were isolated from the root of Corydalis yanhusuo L. collected from Wuchang, Heilongjiang Province, northeast China. Phylogenetic analysis of 16S rRNA gene sequences showed that the two strains are closely related to one another (99.8 % similarity), and had the closest relationship with Sphaerisporangium cinnabarinum JCM 3291(T) (98.7, 98.6 %), Sphaerisporangium flaviroseum YIM 48771(T) (98.6, 98.6 %), Sphaerisporangium melleum JCM 13064(T) (98.5, 98.4 %) and Sphaerisporangium dianthi NEAU-CY18(T) (98.4, 98.4 %). DNA-DNA hybridization value between strains NEAU-YHS12 and NEAU-YHS15(T) was 82 ± 1.4 %, and the values between the two strains and the closely related type strains were well below 70 %. The two strains also shared a number of phenotypic characteristics that were distinct from the closely related species. Both strains were observed to contain MK-9(H6), MK-9(H4) and MK-9(H2) as the detected menaquinones. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. The phospholipid profiles were found to contain diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, phosphoglycolipid, and an unidentified phospholipid. The major fatty acids were identified as iso-C16:0, C17:1 ω7c, C18:0 and iso-C15:0. On the basis of the genetic and phenotypic properties, it is proposed that strains NEAU-YHS15(T) and NEAU-YHS12 be classified as representatives of a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium corydalis sp. nov is proposed. The type strain is NEAU-YHS15(T) (CGMCC 4.7148(T) = DSM46732(T)).

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Sphaerisporangium flaviroseum sp. nov. and Sphaerisporangium album sp. nov., isolated from forest soil in China

Cited by 29 publications

References 25 publications

Sphaerisporangium siamense sp. nov., an actinomycete isolated from rubber-tree rhizospheric soil

Sphaerisporangium siamense sp. nov., an actinomycete isolated from rubber-tree rhizospheric soil

Sinosporangium album gen. nov., sp. nov., a new member of the suborder Streptosporangineae

Sphaerisporangium corydalis sp. nov., isolated from the root of Corydalis yanhusuo L.

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