Spermiometrics: the method and its clinical application

Scheiber, K.; Schmassmann, Adrian; Rohr, H.P.; Oefner, Peter J.; Gotwald, M.; Bartsch, Georg

doi:10.1007/bf00326396

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…During the 1980–1990s, many groups worked on the definition of sperm head morphometry with semi-automatic image analysis systems 41424344454647. From the beginning, the introduction of commercial Computer-Assisted/Aided Semen Analysis (CASA) technology for morphological analysis proved useful in different species for determining sperm quality 22344849505152535455.…”

Section: Objective Approaches To Sperm Morphology Assessmentmentioning

confidence: 99%

Foreword to Sperm morphometrics today and tomorrow special issue in Asian Journal of Andrology

Soler

Cooper

2016

Asian J Androl

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Early in his investigations, Leeuwenhoek (1670s)1 deduced that spermatozoa were alive and an integral part of semen, rather than artifacts or parasites. He eventually observed spermatozoa in the semen of men, dogs, horses, birds, fishes, amphibians, molluscs, and many insects, and concluded that they must be a universal feature of male reproduction. The huge differences in sperm form among species have been discussed in relation to evolutionary changes dictated by the egg and its investments.2 Spallanzani (1800s)1 was the first scientist to develop successful methods for artificial insemination, first with amphibians and later with dogs. With these experiments, he showed that physical contact between intact spermatozoa and ova was necessary to achieve the fertilization. Some years later (1820s), Prévost and Dumas1 performed the defining experiment to identify correctly the function of spermatozoa in reproduction.

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Section: Objective Approaches To Sperm Morphology Assessmentmentioning

confidence: 99%

Foreword to Sperm morphometrics today and tomorrow special issue in Asian Journal of Andrology

Soler

Cooper

2016

Asian J Androl

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Computer‐Assisted Human Semen Analysis Sampling Errors and Reproducibility

Ginsburg

Moghissi

Abel

1988

Journal of Andrology

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Videomicrographic computer-automated semen analysis systems allow quantitative description of sperm motility, velocity, progression, and head movement amplitude and frequency with unprecedented ease. The minimum number of spermatozoa needed for stable results, the variability of measurements and optimum methods of sampling the ejaculate were determined for one such system (Cell-Soft, CRYO Resources, New York, NY). Sampling a minimum of 225 spermatozoa yields stable measurements, and analyzing four microscope fields in triplicate provides data with the lowest coefficient of variation. The variability attributable to the instrument itself was acceptable for all measurements (6.2% to 15.4%) except mean amplitude of lateral head displacement. Limitations of these results and the potential utility of videomicrographic sperm movement analysis are discussed.

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Spermiometrics: the method and its clinical application

Cited by 2 publications

References 14 publications

Foreword to Sperm morphometrics today and tomorrow special issue in Asian Journal of Andrology

Foreword to Sperm morphometrics today and tomorrow special issue in Asian Journal of Andrology

Computer‐Assisted Human Semen Analysis Sampling Errors and Reproducibility

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