Spermiogenesis of rat, mouse, hamster and guinea pig as revealed by the “periodic acid‐fuchsin sulfurous acid” technique

Leblond, C. P.; Clermont, Y.

doi:10.1002/aja.1000900202

Cited by 722 publications

(346 citation statements)

References 20 publications

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“…Its interpretation in terms of the stages of spermatogenesis irradiated is more problematical. Fortunately there has recently been a number of investigations on the timing of the various stages of spermatogenesis (Leblond and Clermont, 1952 ; Edwards, 1955, andOakberg, 1957). These have been synthesised into a single scheme by Sirlin and Edwards (i7).…”

Section: Sperm Sample Equated To Stage Irradiatedmentioning

confidence: 99%

Mutagenic sensitivity of maturing germ cells in the male mouse

Bateman

1958

Heredity

126

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Section: Sperm Sample Equated To Stage Irradiatedmentioning

confidence: 99%

Mutagenic sensitivity of maturing germ cells in the male mouse

Bateman

1958

Heredity

126

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“…DNA histograms of the stages of the mouse seminiferous epithelial cycle closely resembled corresponding histograms of the rat cycle (28), reflecting the anatomical similarity of the organization of the seminiferous epithelium in these species (14). There were, however, differences in the DNA histograms between these species.…”

Section: Discussionmentioning

confidence: 64%

“…Key terms: Mouse, flow cytometry, spermatogenesis, testis Mouse spermatogenic cells in the seminiferous epithelium are arranged in distinct cell associations called stages of the cycle of seminiferous epithelium (14,21). Organization of the mouse seminiferous epithelium is similar to that in the rat, but instead of 14 stages, only 12 stages are distinguished in the mouse (21).…”

mentioning

confidence: 99%

DNA flow cytometric analysis of mouse seminiferous epithelium

Toppari¹,

Bishop²,

Parker³

et al. 1988

Cytometry

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In order to provide a basis for quantitative studies of murine spermatogenesis, we performed a DNA flow cytometric analysis on the mouse seminiferous tubules isolated at defined stages of the epithelial cycle by transillumination-assisted microdissection. Accurate stage identification was performed by examining spermatids in the adjacent tubule segments by phase-contrast microscopy. For flow cytometry, suspension of nuclei of spermatogenic cells was obtained by detergent treatment of isolated seminiferous tubules, and fresh samples were stained with propidium iodide.DNA histograms of the 12 stages of the mouse seminiferous epithelial cycle varied in a stage-specific manner. DNA histograms of stages I-VIII of the cycle were characterized by a hypofluorescent haploid peak, the location of which changed with the decreasing DNA dye (propidium iodide)-binding capacity of elongated spermatids. The absence of the hypohaploid peak and the high ratio of the cells with 4C amount of DNA to the cells with 1C amount of DNA characterized stages IX-XI of the cycle. Stage XI1 showed a high 2C peak, owing to a large population of secondary spermatocytes arisen from the first meiotic division. By using fluorescent beads as an internal volume standard cell numbers in defined stages were determined. These data provide a basis for quantitative studies of mouse spermatogenesis.

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“…The numbers of primary spermatocytes and round spermatids were scored at stages I-VIII of the seminiferous cycle in 15 transverse cross sections of the seminiferous tubules (Leblond and Clermont, 1952;Oakberg, 1956). The Abercrombie correction (Abercrombie, 1946) was applied to the cell counts to calculate the mean spermatid-to-spermatocyte ratio (SSR).…”

Section: Fertility Estimatesmentioning

confidence: 99%

Fertility assessment in hybrids between monobrachially homologous Rb races of the house mouse from the island of Madeira: implications for modes of chromosomal evolution

et al. 2010

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The speciation model of divergence by monobrachially homologous fusions (that is, with one arm in common) benefits from a wide conceptual acceptance, because heterozygotes between populations carrying such fusions suffer from high levels of meiotic dysfunction. The same meiotic configurations can also be generated by WART (whole-arm reciprocal translocation), rearrangements that are known to occur in mammals. Estimating the disadvantage of heterozygotes carrying monobrachially homologous fusions is required to evaluate the relevance of this mode of chromosomal evolution in diversification and speciation. House mice are an excellent study models because chromosomal races exist carrying monobrachially homologous fusions, and WARTs have been documented in this species. The fertility of heterozygote mice carrying the smallest number of monobrachially homologous fusions (that is, a chain of four chromosomes, C4) was investigated in laboratory-bred hybrids between two parapatric chromosomal races from the island of Madeira. Meiotic nondisjunction analyses and histological sections of testes showed that aneuploidy (16.7%) and germ cell death (50.9%) rates reached significantly higher mean values in hybrids than in homozygotes. In females, however, the histological analysis of ovarian follicle parameters revealed no significant differences between hybrid and homozygous individuals. Overall, the reproductive assays indicated that these C4-carrying hybrids were not sterile but showed an approximately 50% decrease in fertility compared to homozygous parental mice. Implications for modes of chromosomal evolution involving monobrachially homologous fusions are discussed.

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Spermiogenesis of rat, mouse, hamster and guinea pig as revealed by the “periodic acid‐fuchsin sulfurous acid” technique

Cited by 722 publications

References 20 publications

Mutagenic sensitivity of maturing germ cells in the male mouse

Mutagenic sensitivity of maturing germ cells in the male mouse

DNA flow cytometric analysis of mouse seminiferous epithelium

Fertility assessment in hybrids between monobrachially homologous Rb races of the house mouse from the island of Madeira: implications for modes of chromosomal evolution

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