Spermiogenesis of Eupyrene Sperm in Prepupae, Pupae, and Adults of Heliothis virescens (Lepidoptera: Noctuidae): an Ultrastructural Study

Lachance, Leo E.; Olstad, D Geneva

doi:10.1093/aesa/81.2.292

Cited by 13 publications

(7 citation statements)

References 16 publications

(21 reference statements)

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“…1) (Klowden, 2004(Klowden, , 2013. In most Lepidoptera, at the end of their larval development (pre-pupal stage), the paired kidney-shaped testes fuse into a single organ that then undergoes torsion such that pupae and adult males possess a single gonad consisting of eight twisted chambers, known as testicular follicles (TF), (Cholodkovsky, 1880;Drecktrah, 1966;Jones, 1973;LaChance & Olstad, 1988;Pereira & Santos, 2015). It has been clearly shown, at least in some species, that simultaneously with the testis Fig.…”

Section: Introductionmentioning

confidence: 99%

Effects of larval diapause and the juvenile hormone analog, fenoxycarb, on testis development and spermatogenesis in the wax moth, Galleria mellonella (Lepidoptera: Pyralidae)

Bębas¹,

Cymborowski²,

Kazek³

et al. 2018

Eur. J. Entomol.

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http://www.eje.cz fusion-torsion process, spermiogenesis of developmentally advanced spermatids is initiated, leading to the appearance of mature spermatozoa bundles that increase in number with the progression of metamorphosis (Chaudhury & Raun, 1966; Nowock, 1972, 1973; Jones et al., 1984; Corsato-Alvarenga et al., 1987). The population of primary germ cells usually decreases as the germarium in each TF degenerates or at least decreases and is less active (Sonoli & Hooli, 1992; Witalis & Godula, 1993). In insects, the germ cells derived from single primary spermatogonia remain interconnected after division and are enveloped in somatic cells (known as cyst cells), thus forming a structure consisting of cysts that ultimately form elongated bundles of spermatozoa (

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Section: Introductionmentioning

confidence: 99%

Effects of larval diapause and the juvenile hormone analog, fenoxycarb, on testis development and spermatogenesis in the wax moth, Galleria mellonella (Lepidoptera: Pyralidae)

Bębas¹,

Cymborowski²,

Kazek³

et al. 2018

Eur. J. Entomol.

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“…Apyrene (anucleated) spermatozoa have a dense cap, two mitochondrial derivatives, and an axoneme with a "9+9+2" microtubular pattern (França & Báo 2000, Mancini & Dolder 2004a, Friedlander et al 2005, Mancini et al 2005, Dallai 2014). The spermatogenesis process occurs inside of the testes; the larvae contain paired organs, but the organ may fuse together into a single structure or remain separated in adults (LaChance & Olstad 1988, Friedlander et al 2005.…”

Section: Introductionmentioning

confidence: 99%

Testis Morphology and Dichotomic Spermatogenesis in the Sugarcane Borer Diatraea saccharalis (F.) (Lepidoptera: Crambidae)

Pereira

Santos

2015

Neotrop Entomol

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In the present study, we describe the testis morphology and spermatogenesis of the sugarcane borer, one of the most destructive pests of sugarcane, using light, transmission, and scanning electron microscopies. Diatraea saccharalis (F.) larvae have paired, kidney-shaped testes that are limited externally and divided into four follicles by tunica cells. The adults have two fused whitish and spherical testes that are divided into eight follicles and are composed of spermatogonia, spermatocyte, spermatid, and sperm bundles. The spermatogonia and spermatocyte cysts are located at the apical extremity of the follicle, while the spermatid cysts and sperm bundles are located at the basal extremity of the follicle. Inside of the cysts, which are formed by the somatic cells, the germ line cells develop synchronously and are interconnected by cytoplasmic bridges. Each cyst contains only apyrene or eupyrene, and none of the cysts contain both of the cells. The inner part of the apyrene cyst consists of an electron-dense cap as opposed to a nucleus. The eupyrene sperm contains a nucleus, acrosome, and reticular and lacinate appendages. Both of the sperm types have a "9 + 9 + 2" axoneme and two mitochondrial derivatives at the tail region. The spermatogenesis process and spermatozoa morphology are similar to that of other Lepidoptera.

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“…During the prepupal or pupal period, the two separated testes fuse together to form a single one [6][7][8]. The testicular fusion has been reported in many lepidopteran insects, such as the Crambidae insects Diatraea saccharalis [7], the Lymantriidae insect Agraulis vanillae [8], the Nymphalidae insect Dione juno [8], the Noctuidae insects Heliothis zea [9], Heliothis virescens [10], and S. litura [11] and Ostrinia nubilalis [12], the Sphingidae insect Manduca sexta [13]. Most of these insects are important agricultural pests, causing extensive damage to cotton, soybean, tobacco, cruciferous vegetables [14,15].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of the testicular fusion in Spodoptera litura

Chang

Liu

et al. 2020

BMC Genomics

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Background: Lepidoptera is one group of the largest plant-feeding insects and Spodoptera litura (Lepidoptera: Noctuidae) is one of the most serious agricultural pests in Asia countries. An interesting and unique phenomenon for gonad development of Lepidoptera is the testicular fusion. Two separated testes fused into a single one during the larva-to-pupa metamorphosis, which is believed to contribute to sperm production and the prevalence in field. To study the molecular mechanism of the testicular fusion, RNA sequencing (RNA-seq) experiments of the testes from 4-day-old sixth instar larvae (L6D4) (before fusion), 6-day-old sixth instar larvae (L6D6, prepupae) (on fusing) and 4-day-old pupae (P4D) (after fusion) of S. litura were performed. Results: RNA-seq data of the testes showed that totally 12,339 transcripts were expressed at L6D4, L6D6 and P4D stages. A large number of differentially expressed genes (DEGs) were up-regulated from L6D4 to L6D6, and then more genes were down-regulated from L6D6 to P4D. The DEGs mainly belongs to the genes related to the 20E signal transduction pathway, transcription factors, chitin metabolism related enzymes, the families of cytoskeleton proteins, extracellular matrix (ECM) components, ECM-related protein, its receptor integrins and ECM-remodeling enzymes. The expression levels of these genes that were up-regulated significantly during the testicular fusion were verified by qRT-PCR. The matrix metalloproteinases (MMPs) were found to be the main enzymes related to the ECM degradation and contribute to the testicular fusion. The testis was not able to fuse if MMPs inhibitor GM6001 was injected into the 5th abdomen region at L6D6 early stage. Conclusions: The transcriptome and DEGs analysis of the testes at L6D4, L6D6 and P4D stages provided genes expression information related to the testicular fusion in S. litura. These results indicated that cytoskeleton proteins, ECM-integrin interaction genes and ECM-related proteins were involved in cell migration, adhesion and fusion during the testicular fusion. The ECM degradation enzymes MMPs probably play a critical role in the fusion of testis.

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Spermiogenesis of Eupyrene Sperm in Prepupae, Pupae, and Adults of Heliothis virescens (Lepidoptera: Noctuidae): an Ultrastructural Study

Cited by 13 publications

References 16 publications

Effects of larval diapause and the juvenile hormone analog, fenoxycarb, on testis development and spermatogenesis in the wax moth, Galleria mellonella (Lepidoptera: Pyralidae)

Effects of larval diapause and the juvenile hormone analog, fenoxycarb, on testis development and spermatogenesis in the wax moth, Galleria mellonella (Lepidoptera: Pyralidae)

Testis Morphology and Dichotomic Spermatogenesis in the Sugarcane Borer Diatraea saccharalis (F.) (Lepidoptera: Crambidae)

Transcriptomic analysis of the testicular fusion in Spodoptera litura

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