Spermatozoa quality and sperm lipid composition in intensively cultured and wild burbot (Lota lota)

Blecha, Miroslav; Dzyuba, Borys; Boryshpolets, Sergii; Horokhovatskyi, Yevhen; Dadras, Hadiseh; Malinovskyi, Oleksandr; Sampels, Sabine; Policar, T.

doi:10.1016/j.anireprosci.2018.09.011

Cited by 18 publications

(11 citation statements)

References 35 publications

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“…Nevertheless, the spermatozoon motility duration (which is known to be generally shorter for freshwater species and longer for marine fish) at 4 °C was typical of other freshwater species such as the Arctic char ( Salvelinus alpinus ) and the pikeperch ( Sander lucioperca ) in temperatures of 2–5 °C [ 25 , 26 ]. The VCL was well within the range of previous values found for the burbot, but lower than the VCL of trout sperm [ 20 , 27 , 28 ].…”

Section: Discussionsupporting

confidence: 84%

“…Several studies have been conducted to mitigate the population decline directly or indirectly. Most of them have focused on understanding its life cycle, enhancing reproduction in hatcheries, or understanding and improving sperm physiology [ 3 , 16 , 20 , 21 , 22 , 23 , 24 ]. Nevertheless, sperm energy budgeting (production, accumulation, and usage) and its adaptation to raised temperatures were still unexplored before this study commenced.…”

Section: Discussionmentioning

confidence: 99%

“…Fortunately, cellular systems can detoxify themselves [ 43 ]. Burbots have a ω6/ω3 fatty acid (FA) ratio, which is more skewed towards ω6 than ω3: 1.6:1 to ~3:1 (re-calculated from [ 20 ]). Such a proportionately high level of ω6 FAs is known to slow down lipid oxidation and ROS formation [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Existing data on the lipid composition of burbot sperm indicate that the ratio of polyunsaturated fatty acids (PUFAs)/saturated or mono-unsaturated fatty acids (SFAs + MUFAs) is ~1:1 [ 20 ]. Although mitochondrial β-oxidation in fish happens more freely for SFAs and MUFAs, β-oxidation of PUFAs can be quite variable or complicated [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

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Bioenergetic Pathways in the Sperm of an Under-Ice Spawning Fish, Burbot (Lota lota): The Role of Mitochondrial Respiration in a Varying Thermal Environment

Rahi

Dzyuba

Policar

et al. 2021

Biology

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Regarding the sperm of cold-water fish, the contributions of different bioenergetic pathways, including mitochondrial respiration, to energy production at the spawning temperature and its adaptation at the maximum critical temperature (CTmax) are unclear. The roles of glycolysis, fatty acid oxidation, oxidative phosphorylation (OXPHOS) at 4 °C, and OXPHOS at 15 °C for energy production in burbot (Lota lota) spermatozoa were studied by motility and the oxygen consumption rate (OCR) (with and without pathway inhibitors and the OXPHOS uncoupler). At both temperatures, the effects of the inhibitors and the uncoupler on the motility duration, curvilinear velocity, and track linearity were insignificant; in addition, the OCRs in activation and non-activation media differed insignificantly and were not enhanced after uncoupler treatment. After inhibitor treatment in both media, OXPHOS was insignificantly different at the 2, 30, and 60 s time points at 4 °C but was reduced significantly at the 30 and 60 s time points after treatment with sodium azide at 15 °C. In conclusion, for burbot sperm at both the spawning temperature and the CTmax, the energy synthesized via OXPHOS during motility was insufficient. Therefore, the majority of the energy required to sustain motility was derived from pre-accumulated energy produced and stored during the quiescent state of the spermatozoa.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Bioenergetic Pathways in the Sperm of an Under-Ice Spawning Fish, Burbot (Lota lota): The Role of Mitochondrial Respiration in a Varying Thermal Environment

Rahi

Dzyuba

Policar

et al. 2021

Biology

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“…The ratio of n-3 and n-6 PUFAs was considerably higher in sterlet sperm (0.71) compared to stingray sperm (0.41). High amounts of n-3 FAs have already been reported for other externally fertilizing fish species [16,[55][56][57]. Despite these differences, the ratio SFA/(MUFA+PUFA) was nearly identical in sterlet and stingray sperm (sterlet: 0.24, stingray: 0.25).…”

Section: Gas Chromatographic Investigations Of the Lipid Fractionmentioning

confidence: 76%

Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization

et al. 2020

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The lipid composition of sperm membranes is crucial for fertilization and differs among species. As the evolution of internal fertilization modes in fishes is not understood, a comparative study of the sperm lipid composition in freshwater representatives of externally and internally fertilizing fishes is needed for a better understanding of taxa-specific relationships between the lipid composition of the sperm membrane and the sperm physiology. The lipidomes of spermatozoa from stingray, a representative of cartilaginous fishes possessing internal fertilization, and sterlet, a representative of chondrostean fishes with external fertilization, have been studied by means of nuclear magnetic resonance (NMR), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), electrospray MS, gas chromatography-(GC) MS, and thin-layer chromatography (TLC). NMR experiments revealed higher cholesterol content and the presence of phosphatidylserine in stingray compared to sterlet sperm. Unknown MS signals could be assigned to different glycosphingolipids in sterlet (neutral glycosphingolipid Gal-Cer(d18:1/16:0)) and stingray (acidic glycosphingolipid sulpho-Gal-Cer(d18:1/16:0)). Free fatty acids in sterlet sperm indicate internal energy storage. GC-MS experiments indicated a significant amount of adrenic acid, but only a low amount of docosahexaenoic acid in stingray sperm. In a nutshell, this study provides novel data on sperm lipid composition for freshwater stingray and sterlet possessing different modes of fertilization.

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Factors Influencing Milt Quality in Fishes and Its Usefulness to Cryopreservation

Ciereszko

Judycka

Nynca

et al. 2020

Cryopreservation of Fish Gametes

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Spermatozoa quality and sperm lipid composition in intensively cultured and wild burbot (Lota lota)

Cited by 18 publications

References 35 publications

Bioenergetic Pathways in the Sperm of an Under-Ice Spawning Fish, Burbot (Lota lota): The Role of Mitochondrial Respiration in a Varying Thermal Environment

Bioenergetic Pathways in the Sperm of an Under-Ice Spawning Fish, Burbot (Lota lota): The Role of Mitochondrial Respiration in a Varying Thermal Environment

Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization

Factors Influencing Milt Quality in Fishes and Its Usefulness to Cryopreservation

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