Spermatozoa micro ribonucleic acid–34c level is correlated with intracytoplasmic sperm injection outcomes

Cui, Long; Li, Fang; Shi, Biwei; Qiu, Sunquan; Ye, Yinghui

doi:10.1016/j.fertnstert.2015.05.003

Cited by 46 publications

(48 citation statements)

References 32 publications

(47 reference statements)

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“…In maternal plasma, the upregulation of miR-34c expression was directly related to good-quality embryos in D3. [ 35 ] There was a strong correlation between miR-34c expression level in sperm and the results of intracytoplasmic sperm injection (ICSI) treatment in cattle. [ 35 ] MiR-34c-positive patients are more likely to have good-quality embryo, implantation, pregnancy, and live birth.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Dysregulation and Steroid Hormone Receptor Expression in Uterine Tissues of Rats with Endometriosis during the Implantation Window

Cai

Zhu

et al. 2018

Chinese Medical Journal

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Background:Estrogen receptor (ER) and progesterone receptor (PR) are involved in endometriosis, but the involvement of microRNAs (miRNAs) is unknown. The aim of the study was to explore the correlation between miRNA and ER/PR in uterine tissues of rats with endometriosis during the implantation window.Methods:Twenty female Sprague-Dawley rats were randomized in three groups: endometriosis (n = 7), fat tissue control (n = 6), and normal (n = 7) groups. The female rats were mated and sacrificed on day 5 (implantation). Uterine tissues were obtained for hematoxylin-eosin staining, immunohistochemistry, and miRNA expression. Reverse transcription polymerase chain reaction (RT-PCR) was used to validate the expression of rno-miR-29c-3p, rno-miR-34c-5p, rno-miR-141-5p, rno-miR-24-1-5p, and rno-miR-490-5p.Results:The 475 miRNAs were found to differentially express between the endometriosis and normal control groups, with 127 being upregulated and 348 being downregulated. Expression of five miRNAs (rno-miR-29c-3p, rno-miR-34c-5p, rno-miR-141-5p, rno-miR-24-1-5p, and rno-miR-490-5p) were validated by RT-PCR and found to be differentially expressed among the three groups. Expression of ER and PR proteins (immunohistochemistry) in the glandular epithelium and endometrial stroma was significantly different among the three groups (all P < 0.05). Five miRNAs were involved in pathways probably taking part in implantation and fertility.Conclusions:The results suggested that miRNAs, ER, and PR could play important roles in the embryo implantation period of rats with endometriosis. These miRNAs might play a role in endometrial receptivity in endometriosis.

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Section: Discussionmentioning

confidence: 99%

MicroRNA Dysregulation and Steroid Hormone Receptor Expression in Uterine Tissues of Rats with Endometriosis during the Implantation Window

Cai

Zhu

et al. 2018

Chinese Medical Journal

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“…A recent knockout study suggests that the observations could be due to simultaneous inhibition of miR‐34 and miR‐449 by the injected miRNA inhibitor . Interestingly, the levels of miR‐34c in human spermatozoa are positively correlated with better assisted reproduction outcomes by intracytoplasmic sperm injection, and sperm miR‐34c‐5p has been proposed as a noninvasive biomarker for diagnosis of male infertility …”

Section: Is There a Role Of Mirnas In Preimplantation Embryo Developmmentioning

confidence: 99%

MicroRNA and Embryo Implantation

Liu

Niu

et al. 2015

American J Rep Immunol

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Problem In mammals, implantation involves interactions between an activated blastocyst and a receptive endometrium. There are controversies on the role of microRNAs in preimplantation embryo development. The actions of endometrial microRNAs on implantation are beginning to be understood. Method of Study Review of literature on microRNAs in preimplantation embryos and endometrium. Results Emerging evidence suggests a role of microRNAs in blastocyst activation and implantation. Differential expression of microRNAs is found between receptive and non‐receptive endometria. Members of the let‐7, miR‐200, miR‐30 families, and the miR‐17‐92 clusters are more commonly found to be associated with endometrial receptivity. Experimental studies show that the targets of the differentially expressed microRNAs affect endometrial receptivity, decidualization, and embryo implantation. Free and exosome/microvesicle containing microRNAs have been detected in human and ovine uterine luminal fluid (ULF). They may serve as mediators of embryo–endometrium dialog. Some observations suggest that the microRNAs in ULF may be used as biomarkers in infertility treatment. Conclusion MicroRNAs in endometrium and blastocysts are involved in the implantation process.

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“…The morphological evaluation of all the embryos was performed on day 3 after oocyte retrieval. A good-quality embryo was defined as described previously (Cui et al, 2015): 7-9 cells with equally sized, mononucleated blastomeres and <10% fragmentation. A clinical pregnancy was identified by a positive plasma β-human chorionic gonadotrophin (β-HCG) concentration and the visualization of a fetal heartbeat using ultrasound after 6 weeks.…”

Section: In Vitro Fertilizationmentioning

confidence: 99%

Sperm Ribosomal DNA Promoter Methylation Levels Are Correlated With Paternal Aging and May Relate With in vitro Fertilization Outcomes

Tian

et al. 2020

Front. Genet.

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The impact of aging on reproductive outcomes has received considerable critical attention; however, there is much less information available on the effects of paternal age compared to the effects of maternal age. In this study, methylation levels of sperm rDNA promoter regions and Long Interspersed Nucleotide Element 1 (LINE-1) were measured using pyrosequencing and fertilization, day 3 good-quality embryo, pregnancies, and implantation results were assessed. We observed significantly increasing levels of DNA methylation in the sperm rDNA promoter regions with age based on stratifying the samples by age alone (P = 0.0001) and performing linear regression analysis (P < 0.0001). Meanwhile, no statistically significant correlations were observed between global LINE-1 methylation with age. No statistically significant correlations were observed between sperm rDNA promoter methylation levels and either the day 3 goodquality embryo rate or clinical pregnancy rate. In contrast, the correlation between sperm rDNA promoter methylation levels and fertilization (2 pronuclei) rate was nearly significant (P = 0.0707), especially the methylation levels of some individual CpG units (CpG_10, P = 0.0176; CpG_11, P = 0.0438; CpG_14, P = 0.0232) and rDNA promoter methylation levels measured using primerS2 (P = 0.0513). No significant correlation was found between sperm rDNA promoter methylation levels and fertilization rates (2 pronuclei, 1 pronuclei, and 1 polypronuclei). Our results demonstrate that sperm are susceptible to age-associated alterations in methylation levels of rDNA promoter regions, suggesting that sperm rDNA promoter methylation levels can be applied to DNA methylation-based age prediction, and that the aberrant methylation of rDNA promoters may be partially responsible for enhanced disease susceptibility of offspring sired by older fathers. Methylation levels of sperm rDNA promoter regions may correlate with polypronuclei rates of IVF programs.

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Spermatozoa micro ribonucleic acid–34c level is correlated with intracytoplasmic sperm injection outcomes

Abstract: Our results demonstrate that miR-34c levels in spermatozoa are correlated with ICSI outcomes, suggesting that paternal miR-34c may play a role in the early phases of embryonic development. Levels of MiR-34c in human spermatozoa may be used as an indicator for ICSI outcomes.

Cited by 46 publications

References 32 publications

MicroRNA Dysregulation and Steroid Hormone Receptor Expression in Uterine Tissues of Rats with Endometriosis during the Implantation Window

MicroRNA Dysregulation and Steroid Hormone Receptor Expression in Uterine Tissues of Rats with Endometriosis during the Implantation Window

MicroRNA and Embryo Implantation

Sperm Ribosomal DNA Promoter Methylation Levels Are Correlated With Paternal Aging and May Relate With in vitro Fertilization Outcomes

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