Spermatogonial stem cells: updates from specification to clinical relevance

Sharma, Swati; Wistuba, Joachim; Pock, Tim; Schlatt, Stefan; Neuhaus, Nina

doi:10.1093/humupd/dmz006

Cited by 95 publications

(86 citation statements)

References 170 publications

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“…Recently, we analysed the spermatogonial potential of various mammalian species. Data from these analyses support this concept as the spermatogonial potential appears conserved during evolution and was similarly balanced within all species analysed (Sharma et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Ageing in men with normal spermatogenesis alters spermatogonial dynamics and nuclear morphology in Sertoli cells

et al. 2019

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Background: Ageing in men is believed to be associated with fertility decline and elevated risk of congenital disorders for the offspring. The previous studies also reported reduced germ and Sertoli cell numbers in older men. However, it is not clear whether ageing in men with normal spermatogenesis affects the testis and germ cell population dynamics in a way sufficient for transmitting adverse age effects to the offspring. Objectives: We examined men with normal spermatogenesis at different ages concerning effects on persisting testicular cell types, that is the germ line and Sertoli cells, as these cell populations are prone to be exposed to age effects. Material and methods: Ageing was assessed in testicular biopsies of 32 patients assigned to three age groups: (i) 28.8 AE 2.7 years; (ii) 48.1 AE 1 years; and (iii) 70.9 AE 6.2 years, n = 8 each, with normal spermatogenesis according to the Bergmann-Kliesch score, and in a group of meiotic arrest patients (29.9 AE 3.8 years, n = 8) to decipher potential links between different germ cell types. Besides morphometry of seminiferous tubules and Sertoli cell nuclei, we investigated spermatogenic output/efficiency, and dynamics of spermatogonial populations via immunohistochemistry for MAGE A4, PCNA, CREM and quantified A-pale/A-dark spermatogonia. Results: We found a constant spermatogenic output (CREM-positive round spermatids) in all age groups studied. In men beyond their mid-40s (group 2), we detected increased nuclear and nucleolar size in Sertoli cells, indirectly indicating an elevated protein turnover. From the 7th decade (group 3) of life onwards, testes showed increased proliferation of undifferentiated spermatogonia, decreased spermatogenic efficiency and elevated numbers of proliferating A-dark spermatogonia. Discussion and conclusion: Maintaining normal sperm output seems to be an intrinsic determinant of spermatogenesis. Ageing appears to affect this output and might provoke compensatory proliferation increase in A spermatogonia which, in turn, might hamper germ cell integrity.

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Section: Discussionmentioning

confidence: 97%

Ageing in men with normal spermatogenesis alters spermatogonial dynamics and nuclear morphology in Sertoli cells

et al. 2019

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“…The cryopreserved testicular cells or tissue could be used for prepubertal human male fertility preservation with different approaches that are already used in rodents [27][28][29][30]. The main limitation of using autologous testicular tissue or cells in human therapy from cancer patients is the possibility of the presence of residual cancer cells, which may restore and evoke the disease.…”

Section: Cryopreservation Of Human Testicular Tissuementioning

confidence: 99%

“…In humans, it was reported that cryopreserved testicular cells from non-Hodgkin's lymphoma patients were transplanted into their testes after recovery from cancer [55,56]; however, follow-up for these patients was not reported. Xeno-germ cell transplantation of baboons, marmosets, and humans into mice only colonized, but did not differentiate [28]. In addition, a limitation of xeno-germ cell transplantation is the risk of possible retrovirus transmission to humans if the developed cells are used for fertility therapy.…”

Section: Germ Cell Transplantationmentioning

confidence: 99%

Approaches and Technologies in Male Fertility Preservation

Huleihel

Lunenfeld

2020

IJMS

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Male fertility preservation is required when treatment with an aggressive chemo-/-radiotherapy, which may lead to irreversible sterility. Due to new and efficient protocols of cancer treatments, surviving rates are more than 80%. Thus, these patients are looking forward to family life and fathering their own biological children after treatments. Whereas adult men can cryopreserve their sperm for future use in assistance reproductive technologies (ART), this is not an option in prepubertal boys who cannot produce sperm at this age. In this review, we summarize the different technologies for male fertility preservation with emphasize on prepubertal, which have already been examined and/or demonstrated in vivo and/or in vitro using animal models and, in some cases, using human tissues. We discuss the limitation of these technologies for use in human fertility preservation. This update review can assist physicians and patients who are scheduled for aggressive chemo-/radiotherapy, specifically prepubertal males and their parents who need to know about the risks of the treatment on their future fertility and the possible present option of fertility preservation.

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“…Возможность восстановления фертильности у мужчин имеет большой потенциал в фундаментальной и прикладной науке [15,50]. Развитие культуральных методов может дать будущую надежду на сохранение фертильности в случаях без других вариантов, например, у препубертатных пациентов, больных раком.…”

Section: возможности использования сперматогониальных стволовых клетоunclassified

Possible use of spermatogonial stem cells in the treatment of male infertility

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Скалецкая

Sevastianov

2020

RJTAO

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Spermatogonial stem cells, which are already present at birth in the testicles, are the progenitors of male gametes. These cells cannot produce mature sperm before puberty due to their dependence on hormonal stimuli. This feature of the reproductive system limits preservation of fertility only to males who can produce an ejaculate. Therefore, the use of cancer treatment which can lead to fertility loss has made sperm cryopreservation a standard practice. Prepubertal cancer boys – who are prescribed chemotherapy that is toxic to their reproductive system – are deprived of this fertility management procedure. This review focuses on the problem of obtaining and preserving spermatogonial stem cells for future transplantation to restore spermatogenesis. Development of these methods is becoming increasingly urgent due to higher survival rates in childhood cancer over the past decades thanks to improvements in diagnosis and effective treatment. Restoring and preserving fertility using spermatogonial stem cells may be the only option for such patients.

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Spermatogonial stem cells: updates from specification to clinical relevance

Cited by 95 publications

References 170 publications

Ageing in men with normal spermatogenesis alters spermatogonial dynamics and nuclear morphology in Sertoli cells

Ageing in men with normal spermatogenesis alters spermatogonial dynamics and nuclear morphology in Sertoli cells

Approaches and Technologies in Male Fertility Preservation

Possible use of spermatogonial stem cells in the treatment of male infertility

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