Spermatogonia survival in young ram lambs following irradiation, Busulfan or thermal treatment

Olejnik, Jeanette; Suchowerska, Natalka; Herrid, Muren; Jackson, Michael; Hinch, G. N.; Hill, Jonathan R.

doi:10.1016/j.smallrumres.2018.07.017

Cited by 6 publications

(9 citation statements)

References 45 publications

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“…While busulfan treatment may appear practical in field conditions (as no expensive equipment is required), it has a clear disadvantage related to its systemic toxicity. The dose-dependent species-specific systemic toxicity of busulfan during treatment was demonstrated in pigs [138] and lambs [139]; therefore, researchers must be aware of the busulfan-related toxicity danger. In lambs, a dose of 8 mg/kg induced thin diarrhea with lethargy and a lack of appetite beginning at 5 days post busulfan injection; the authors suggested using reduced doses of busulfan [139].…”

Section: Ssc Transplantation: Fundamental For the Manipulation Of Spementioning

confidence: 99%

Manipulation of spermatogonial stem cells in livestock species

Savvulidi

Ptáček

Vargová

et al. 2019

J Animal Sci Biotechnol

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We are entering an exciting epoch in livestock biotechnology during which the fundamental approaches (such as transgenesis, spermatozoa cryopreservation and artificial insemination) will be enhanced based on the modern understanding of the biology of spermatogonial stem cells (SSCs) combined with the outstanding recent advances in genomic editing technologies and in vitro cell culture systems. The general aim of this review is to outline comprehensively the promising applications of SSC manipulation that could in the nearest future find practical application in livestock breeding. Here, we will focus on 1) the basics of mammalian SSC biology; 2) the approaches for SSC isolation and purification; 3) the available in vitro systems for the stable expansion of isolated SSCs; 4) a discussion of how the manipulation of SSCs can accelerate livestock transgenesis; 5) a thorough overview of the techniques of SSC transplantation in livestock species (including the preparation of recipients for SSC transplantation, the ultrasonographic-guided SSC transplantation technique in large farm animals, and the perspectives to improve further the SSC transplantation efficiency), and finally, 6) why SSC transplantation is valuable to extend the techniques of spermatozoa cryopreservation and/or artificial insemination. For situations where no reliable data have yet been obtained for a particular livestock species, we will rely on the data obtained from studies conducted in rodents because the knowledge gained from rodent research is translatable to livestock species to a great extent. On the other hand, we will draw special attention to situations where such translation is not possible. Electronic supplementary material The online version of this article (10.1186/s40104-019-0355-4) contains supplementary material, which is available to authorized users.

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Section: Ssc Transplantation: Fundamental For the Manipulation Of Spementioning

confidence: 99%

Manipulation of spermatogonial stem cells in livestock species

Savvulidi

Ptáček

Vargová

et al. 2019

J Animal Sci Biotechnol

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“…In our study, the testicular injection of shal rams with 1, 2 and 4 mg/kg of busulfan resulted in a dose dependent decrease in testis size and also spermatocytes population after 5 weeks of treatments. Systemic injection of busulfan reduced endogenous spermatogonia in the pre-pubertal ram (Olejnik et al, 2005), however, Olejnik et al (2018) reported that spermatogonia numbers were not significantly reduced by using 4 mg/kg busulfan intravenously, although 27% were changed, indicating that the power of their experiment was insufficient to detect a difference (Olejnik et al, 2018).…”

Section: Discussionmentioning

confidence: 98%

“…Nevertheless, animals in the 4 mg/kg group showed only mild clinical effects that were not life-threatening (Olejnik et al, 2005). However, Olejnik et al (2018) used a dose of 4 mg/kg in their experiment and reported a dramatic falls in white blood cells and platelets numbers, which were recovered within 3 weeks (Olejnik et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The results of Olejnik et al (2018) revealed that sheep are more sensitive to the toxic effects of busulfan compared to other species. While 4 mg/kg caused a small effect on spermatogonia, higher doses of it would carry a very high risk of animal health due to bone marrow toxicity (Olejnik et al, 2018). Susceptibility to busulfan varies even in pig breeds; however, different types of mice have been shown to be resistant to it (Ganguli et al, 2016;Qin et al, 2016b).…”

Section: Discussionmentioning

confidence: 99%

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Spermatogonial stem cell survival in ram lambs following busulfan treatment

et al. 2020

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To clarify the effect of busulfan on the depletion of spermatogonial stem cells (SSCs) from shal rams testis, in the first experiment, lambs were treated by intraperitoneal injection with 4 mg/kg busulfan. In the second experiment, different concentrations of busulfan (1, 2 and 4 mg/kg) were injected directly into both sides of the left testis. The testes of 8 lambs were collected by standard castration procedure for histological analysis five weeks after the treatments and the left testis of remaining lambs were collected after eight weeks and a two-time enzymatic digestion process was used to isolate SSCs. The results showed that all rams that had received intraperitoneal injections of busulfan died. But by testicular injecting of same dose of the drug, 40% of the animals died. The testicular injection of rams with 1, 2 and 4 mg/kg of busulfan resulted in a dose dependent decrease in testis size and also spermatocytes population after 5 weeks of treatments. From the results of colony formation 8 weeks after treatment with busulfan, it can be concluded that only in 1 and 2 mg/kg of busulfan, recovery of endogenous germ cells was performed. In conclusion, the results demonstrated that intra-testicular injections of busulfan (2 mg/kg) reduced spermatocytes population in ram testis within 5 weeks of treatments, and this effect was reversible within 8 weeks of injection. However, it was not recommended to inject 4 mg/kg busulfan into the peritoneal cavity or testis of lambs based on its side effects.

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