Spermatogonia

Fayomi, A; David, Sherin; Doungkamchan, Chatchanan; Orwig, Kyle E.

doi:10.1016/b978-0-12-801238-3.64424-0

Cited by 2 publications

(4 citation statements)

References 131 publications

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“…Immunohistochemistry of cytosine modifications in human testes sections showed that levels of 5hmC are decreasing as spermatogenesis proceeds, while 5mC levels remain constant, indicate that during spermatogenesis active DNA demethylation becomes downregulated leading to a conservation of the methylation marks in mature sperm (Nettersheim et al, 2013). To estimate the 5mC and 5hmC levels in spermatogonia cells, we performed co-immunostaining using promyelocytic leukemia zinc-finger (PLZF) as a marker of undifferentiated spermatogonia (Buaas et al, 2004;Costoya et al, 2004;Hobbs et al, 2012), a less abundant spermatogonia population containing spermatogonial stem cells (SSCs) (Fayomi and Orwig, 2018). Spermatogonia cells are enriched in neonatal mouse testis , but very few cells in tubules were stained positive for PLZF in adult testis, as shown in a recent study (Grive et al, 2019).…”

Section: Tet1 Deficiency Reduces Male Fertility With Agementioning

confidence: 99%

“…Adult stem cells play essential roles in maintenance of tissue homeostasis and regeneration. In mouse testis, SSCs are able to self-renew and differentiate, maintaining the continuous spermatogenesis to produce spermatozoa (Fayomi and Orwig, 2018;Kubota and Brinster, 2018). Because some complete degeneration of the seminiferous epithelium with a Sertoli cell-only phenotype was observed in Tet1 À/À testis and Tet1 was expressed in spermatogonia (containing SSCs) , this inspired us to hypothesize that loss of Tet1 might impact self-renewal of SSCs, causing a progressive loss of germ cells with age.…”

Section: Tet1 Deficiency Causes Progressive Loss Of Germ Cells With Agementioning

confidence: 99%

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Tet1 Deficiency Leads to Premature Reproductive Aging by Reducing Spermatogonia Stem Cells and Germ Cell Differentiation

et al. 2020

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Ten-eleven translocation (Tet) enzymes are involved in DNA demethylation, important in regulating embryo development, stem cell pluripotency and tumorigenesis. Alterations of DNA methylation with age have been shown in various somatic cell types. We investigated whether Tet1 and Tet2 regulate aging. We showed that Tet1-deficient mice undergo a progressive reduction of spermatogonia stem cells and spermatogenesis and thus accelerated infertility with age. Tet1 deficiency decreases 5hmC levels in spermatogonia and downregulates a subset of genes important for cell cycle, germ cell differentiation, meiosis and reproduction, such as Ccna1 and Spo11, resulting in premature reproductive aging. Moreover, Tet1 and 5hmC both regulate signaling pathways key for stem cell development, including Wnt and PI3K-Akt, autophagy and stress response genes. In contrast, effect of Tet2 deficiency on male reproductive aging is minor. Hence, Tet1 maintains spermatogonia stem cells with age, revealing an important role of Tet1 in regulating stem cell aging.

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Section: Tet1 Deficiency Reduces Male Fertility With Agementioning

confidence: 99%

Section: Tet1 Deficiency Causes Progressive Loss Of Germ Cells With Agementioning

confidence: 99%

Tet1 Deficiency Leads to Premature Reproductive Aging by Reducing Spermatogonia Stem Cells and Germ Cell Differentiation

et al. 2020

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“…Another murky issue has been whether findings from rodents apply to humans. This has been particularly vexing given the evidence that human and mouse testes differ significantly in seminiferous epithelium organization, the pattern of SSC differentiation, SSC frequency, and sperm output per gram of tissue (Fayomi and Orwig, 2018).…”

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confidence: 99%

“…A critical question for the future is to determine SSC frequency in the spermatogonial subsets defined by the three groups by purifying these subsets using specific markers and then assaying them for ''SSC activity'' via the xenotransplanation assay (Fayomi and Orwig, 2018). This experiment may lead to sur-prises.…”

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confidence: 99%