Spermatogenic and Haematological effects of Aqueous and Ethanolic Extracts of Hymenocardia acida Stem Bark on Aluminium-Induced Toxicity in Male Wistar Rats

Abstract

This study was designed to determine the spermatogenic and haematological effects of aqueous and ethanolic stem bark extracts of Hymenocardia acida on Aluminium chloride-induced toxicity in male wistar rats. Thirty male albino Wistar rats were used and toxicity was induced using 100 mg/kg body weight (bw) AlCl 3 . The rats were distributed into six groups of five rats each. The drug and extract administration lasted for seven days, thereafter the animals sacrificed. The results indicated that oral dose of 100 mg/kg bw extracts of H. acida did not show any significant (p>0.05) change in testosterone levels when compared with both normal and negative control. Ethanolic extract showed non-significant (p>0.05) increase on Luteinizing hormone (LH) when compared with both normal and negative control group. Administration of AlCl 3 caused a significant (p<0.05) decrease in Follicle Stimulating Hormone (FSH), while the plants extracts caused a non-significant increase when compared with the negative control. Thiobarbituric Acid Reactive Substance (TBARS) increased significantly (p<0.05) in negative control, but decreased significantly in groups treated with aqueous and ethanolic extract of H. acida when compared with the negative control. Induction of AlCl 3 -toxicity caused a non-significant (p>0.05) alterations of most of the haematological parameters evaluated, but administration of the plant extracts was able to mildly ameliorate these effects (especially on RBC and Hb). The result of this study shows AlCl 3 toxicity causes alterations of LH, FSH and TBARS, but aqueous and ethanolic stem bark extracts of H. acida may possibly ameliorate the alteration. Also, AlCl 3 -toxicity mildly influences the synthesis of certain haematological indices, but the extracts may modulate some of this interference.

A comparative study of scopolamine, D-Galactose and AlCl3-induced Alzheimer’s like disease by enhancing hippocampal neurodegeneration and memory impairment in male rats

“…The reduction of RBC, HGB by AlCl 3 in this study suggests the induction of anemia. Also, hematological results indicated that AlCl 3 led to a significantly increased WBC when compared to SCO, D-gala, and control groups (Yakubu et al, 2017). Increase in WBC is suggestive of leukocytosis and anemia as seen from the corresponding decreases in RBC which may indicate the activation of the immune system to form defensive cells (Adedosu et al, 2018).…”

Biochemical evaluation of Ceiba pentandra leaf on the amelioration of testosterone induced-benign prostatic hyperplasia in Wister rats

“…This decrease in FSH may be due to C. pentandra toxic effect on the anterior pituitary gland, causing oxidative damage to the anterior pituitary gland's architecture, responsible for the generation of FSH (glycoprotein hormone) and luteinizing hormone. The decline in testosterone levels in groups administered with aqueous leaf extracts of C. pentandra suggested that its phytochemical composition may reduce the effect of the pituitary gland and testosterone synthesis [19] The effect of aqueous leaf extract of C. pentandra on oxidative stress of testosterone propionate-induced benign prostatic hyperplasia in male rats had a non-significant increase (p > 0.05) in Lactate Dehydrogenase (LDH) activity in Groups 3 to 6 compared to the positive and negative control. Superoxide Dismutase (SOD) concentration in Groups 3 to 6 was significantly decreased (p > 0.05) compared to negative and positive control.…”