Spermatogenesis and karyotypes of three species of water striders (Gerridae, Heteroptera)

Castanhole, Márcia Maria Urbanin; Pereira, Luiz Haroldo; Souza, Hederson Vinícius de; Itoyama, Mary Massumi

doi:10.4238/vol9-3gmr841

Cited by 16 publications

(18 citation statements)

References 17 publications

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“…A peculiarity observed during spermiogenesis of M. brasiliensis was the presence of a vesicle in the spermatids, which has not been observed in the terrestrial (Souza et al, 2007a(Souza et al, ,b, 2008(Souza et al, , 2009) and aquatic species (Castanhole et al, , 2010 studied. Thus, we emphasize that ultrastructural studies are required to describe this structure in more detail in order to clarify its relationship with the formation of sperm.…”

Section: Discussionmentioning

confidence: 86%

“…The round spermatids presented two close corpuscles and during stretching, one corpuscle remained in the head and the other migrated to the initial part of the tail. At the end of the spermiogenesis process, these markings were not visualized (Castanhole et al, 2010).…”

Section: Discussionmentioning

confidence: 95%

“…There have been few cytogenetic studies of aquatic heteropterans (Castanhole et al, , 2010. Of these insects, the nucleolar behavior is only known for the species Limnogonus aduncus, Brachymetra albinerva, Halobatopsis platensis, and Cylindrostethus palmaris (Castanhole et al, , 2010.…”

Section: Introductionmentioning

confidence: 99%

“…Of these insects, the nucleolar behavior is only known for the species Limnogonus aduncus, Brachymetra albinerva, Halobatopsis platensis, and Cylindrostethus palmaris (Castanhole et al, , 2010. The nucleolus is a cellular structure related to biosynthetic activity, therefore, the size and number of nucleoli and nucleolar corpuscles present in the nucleus depends on the functional characteristics of the cells, which may then reflect on metabolic and functional differences (Tavares and Azevedo-Oliveira, 1997;Souza et al, 2007a,b;Alevi et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Study of nucleolar behavior during spermatogenesis in Martarega brasiliensis (Heteroptera, Notonectidae)

Pereira¹,

Alevi²,

Moreira³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Few cytogenetic studies have been undertaken using aquatic heteropterans and the nucleolar behavior of these insects has been described in only four species, Limnogonus aduncus, Brachymetra albinerva, Halobatopsis platensis, and Cylindrostethus palmaris. The nucleolus is a cellular structure related to biosynthetic 8989 Nucleolar behavior in Martarega brasiliensis ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8988-8994 (2015) activity and it exhibits a peculiar behavior in the heteropterans of the Triatominae subfamily; it persists during all stages of meiosis. Thus, this study aims to analyze spermatogenesis in Martarega brasiliensis, with an emphasis on nucleolar behavior. Twenty M. brasiliensis adult males were used and collected from the Municipal reservoir in the city of São José do Rio Preto, São Paulo, Brazil. The species were fixed in methanol:acetic acid (3:1), then dissected, and the testicles were extracted, torn apart, and impregnated with silver ions. During prophase, the nuclei of M. brasiliensis were composed of the nucleolus and nucleolar corpuscles, which varied in number from one to four, emphasizing that this insect has great synthetic activity during meiosis. The analysis of cells in metaphase I showed that M. brasiliensis presents a nucleolar organizing region in at least one autosome. Furthermore, the phenomenon of nucleolar persistence was not observed. All spermatids presented nucleolar markings that varied in number and position according to the stage of elongation. Moreover, it was also possible to highlight the presence of a vesicle in spermatids. Thus, this paper describes the nucleolar behavior of M. brasiliensis and highlights important characteristics during spermatogenesis, thus, increasing the knowledge about the biology of these aquatic heteropterans.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Study of nucleolar behavior during spermatogenesis in Martarega brasiliensis (Heteroptera, Notonectidae)

Pereira¹,

Alevi²,

Moreira³

et al. 2015

Genet. Mol. Res.

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“…It is believed that this characteristic is related to the great energy expenditure that these species need for survival in aquatic environments. Besides, it was observed that the testicular meiotic cells of terrestrial species are much smaller than those of aquatic ones, and when comparing the aquatic species studied nowadays, such cells of the families Gerridae (Castanhole et al, , 2010 and Veliidae are smaller than those of the family Notonectidae. According to Gäde et al (2004), N. glauca (Notonectidae) is very active and constantly swims around in captivity.…”

Section: Discussionmentioning

confidence: 99%

Spermatogenesis of riffle bugs, Rhagovelia whitei and Rhagovelia sp (Veliidae), and backswimmers Martarega sp (Notonectidae)

Castanhole¹,

Pereira²,

Souza³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. We examined the course of spermatogenesis and the meiotic chromosome complements in aquatic species of true bugs, Heteroptera. The chromosome complement of the Veliidae species was 2n = 39 (38A + X0) and 23 (22A + X0) in Rhagovelia whitei and Rhagovelia sp, respectively, and in the species of the Notonectidae (Martarega sp) it was 26 (22A + 2m + XY); all collected from the region of São José do Rio Preto, SP, Brazil. An impressive characteristic of the first analysis was the size of the cells belonging to Martarega sp, which were six times larger than the same cells in Pentatomidae and twice as large as the cells in aquatic Heteroptera (Gerridae). Regarding spermatogenesis, all the species analyzed showed the same pattern: holocentric chromosomes and elongated spermatids with the chromatin distributed evenly along the head. The family Veliidae showed several bodies impregnated with silver nitrate at prophase, while the family Notonectidae displayed only one. The cells of Notonectidae also showed an evident and round body until the end of prophase I and in the family Veliidae the silverimpregnated bodies were disorganized, where the only region visualized was possibly that of the NOR. In metaphase, silver-stained regions were found at the periphery of all chromosomes in Veliidae and at the periphery of some chromosomes in Notonectidae. The spermatids of Veliidae showed a less silver-impregnated vesicle, while Notonectidae showed silver staining only in part of the nuclear membrane. Therefore, families of Heteroptera have some differences and features that can help identify and classify these species.

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Morphology of the male reproductive system and sperm of Leptoglossus zonatus (Dallas, 1852) (Heteroptera: Coreidae)

Paulo,

Rezende,

Dias

et al. 2024

Microscopy Res & Technique

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Taxonomic data on Coreidae have been fragmented over time and need to be revised. Likewise, data related to the development of germ cells and the features of the male reproductive system, including sperm, will contribute to understanding the biological mechanisms of reproduction and the systematics of its representatives. Aiming to provide these data, we describe the morphology of the male reproductive system and spermatozoa of Leptoglossus zonatus using light and transmission electron microscopies, respectively. Each of the two testes is surrounded by a bright red‐pigmented sheath and formed by seven follicles arranged side by side. The two vasa deferentia are filled with individualized sperm, especially in their final portion, which is dilated and curved. After dilation, the vasa deferentia receive the ducts of the accessory glands of mesodermal origin. The other unpaired accessory gland is of ectodermal origin and opens into the ejaculatory duct. Both glandular types are densely coiled and have lumens filled with secreted material. Testicular follicles contain cysts with germ cells at different stages of spermatogenesis, indicating continuous production of gametes throughout adult life. Mature sperm measure around 310 μm long, with a nucleus of 36 μm and a flagellum formed only by an axoneme of 9 + 9 + 2 microtubules and two symmetrical mitochondrial derivatives. Like the sperm of other Heteroptera, the acrosome has a single structure (without perforatorium), there are no accessory bodies in the flagella, and the mitochondrial derivatives are connected to the axonemes, supporting the synapomorphic condition of these characteristics for this suborder of bedbugs.Research Highlights The Leptoglossus zonatus sperm are slender and long, about 310 μm in length, and a nucleus 36 μm long. Spermatogenesis occurs throughout adult life and equally in the seven testicular follicles. The centriole adjunct in L. zonatus sperm does not give rise to accessory bodies. The ectodermal gland produces a filamentous secretion, whereas in the ectodermal sac, the secretion is globular.

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Spermatogenesis and karyotypes of three species of water striders (Gerridae, Heteroptera)

Cited by 16 publications

References 17 publications

Study of nucleolar behavior during spermatogenesis in Martarega brasiliensis (Heteroptera, Notonectidae)

Study of nucleolar behavior during spermatogenesis in Martarega brasiliensis (Heteroptera, Notonectidae)

Spermatogenesis of riffle bugs, Rhagovelia whitei and Rhagovelia sp (Veliidae), and backswimmers Martarega sp (Notonectidae)

Morphology of the male reproductive system and sperm of Leptoglossus zonatus (Dallas, 1852) (Heteroptera: Coreidae)

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