Spermatogenesis and distinctive mature sperm in the giant freshwater prawn, Macrobrachium rosenbergii (De Man, 1879)

Poljaroen, Jaruwan; Vanichviriyakit, Rapeepun; Tinikul, Yotsawan; Phoungpetchara, Ittipon; Linthong, Vichai; Weerachatyanukul, Wattana; Sobhon, Prasert

doi:10.1016/j.jcz.2010.03.002

Cited by 46 publications

(55 citation statements)

References 44 publications

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“…So the acetylation of histone H4 in the central region of the nucleus (together with chromatin fiber), indicates that the gene is active and the DNA is in a loose state, making the mature sperm nucleus non-condensed. The non-condensed sperm nucleus may facilitate fertilization in crustaceans agreeing with Kurtz and Poljaroen's reports (Kurtz et al 2008(Kurtz et al , 2009Poljaroen 2010).…”

Section: Basic Protein In the Late Spermatidsupporting

confidence: 88%

“…Though they vary from species to species, all share the same characteristics such as without flagella, are non-motile, and without a condensed nucleus. In the past a few years, investigators have studied this type of sperm from different angles, such as spermatogenesis (Du et al 1987;Wang et al 1997Wang et al , 1998Zhao et al 1997;Yang et al 1998;Poljaroen et al 2010), acrosomal reaction (Wu et al 2002), and oocyte activation (Wu et al 1999), etc. However, the research on basic proteins during spermatogenesis of the decapoda crustacean has been limited to only a few species.…”

Section: Introductionmentioning

confidence: 99%

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Transition of basic protein during spermatogenesis of Fenneropenaeus chinensis (Osbeck, 1765)

Wang

Kang

et al. 2011

Cytotechnology

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According to the ultrastructural characteristic observation of the developing male germ cells, spermatogenesis of the crustacean shrimp, Fenneropenaeus chinensis, is classified into spermatogonia, primary spermatocytes, secondary spermatocyte, four stages of spermatids, and mature sperm. The basic protein transition during its spermatogenesis is studied by transmission electron microscopy of ammoniacal silver reaction and immunoelectron microscopical distribution of acetylated histone H4. The results show that basic protein synthesized in cytoplasm of spermatogonia is transferred into the nucleus with deposition on new duplicated DNA. In the spermatocyte stage, some nuclear basic protein combined with RNP is transferred into the cytoplasm and is involved in forming the cytoplasmic vesicle clumps. In the early spermatid, most of the basic protein synthesized in the new spermatid cytoplasm is transferred into the nucleus, and the chromatin condensed gradually, and the rest is shifted into the pre-acrosomal vacuole. In the middle spermatid, the nuclear basic protein linked with DNA is acetylated and transferred into the proacrosomal vacuole and assembled into the acrosomal blastema. At the late spermatid, almost all of the basic protein in the nucleus has been removed into the acrosome. During the stage from late spermatid to mature sperm, some de novo basic proteins synthesized in the cytoplasm belt transfer into the nucleus without a membrane and almost all deposit in the periphery to form a supercoating. The remnant histone H4 accompanied by chromatin fibers is acetylated in the center of the nucleus, leading to relaxed DNA and activated genes making the nucleus non-condensed.

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Section: Basic Protein In the Late Spermatidsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Transition of basic protein during spermatogenesis of Fenneropenaeus chinensis (Osbeck, 1765)

Wang

Kang

et al. 2011

Cytotechnology

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“…In the present study, 1 h of lysis and 15 min of denaturation have been considered adequate to eliminate extracellular matrix and to allow DNA relaxation and strand separation in Palaemonidae spermatozoa. These results match with the lack of protamines in the mature sperm of palaemonids as shown in P. serratus (Sellos and Legal 1981) or in Machrobrachuim rosenbergii (Poljaroen et al 2010). Indeed, in contrast to the increasingly condensed chromatin in spermatids of most other invertebrates and vertebrates, the chromatin of these prawns is increasingly decondensed and becomes totally decondensed in the mature sperm (Papathanassiou and King 1984;Poljaroen et al 2010).…”

Section: Optimization Of the Comet Assaysupporting

confidence: 82%

“…These results match with the lack of protamines in the mature sperm of palaemonids as shown in P. serratus (Sellos and Legal 1981) or in Machrobrachuim rosenbergii (Poljaroen et al 2010). Indeed, in contrast to the increasingly condensed chromatin in spermatids of most other invertebrates and vertebrates, the chromatin of these prawns is increasingly decondensed and becomes totally decondensed in the mature sperm (Papathanassiou and King 1984;Poljaroen et al 2010). This particularity presents the advantage to not resort to an enzymatic digestion or a longer lysis (or denaturation) duration for DNA relaxation.…”

Section: Optimization Of the Comet Assaysupporting

confidence: 79%

Assessment of sperm quality in palaemonid prawns using Comet assay: methodological optimization

Erraud

Bonnard

Duflot

et al. 2017

Environ Sci Pollut Res

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The aim of this study was to adapt the Comet assay in spermatozoa of the marine prawn Palaemon serratus to use it as a marker of sperm quality. Indeed, due to the characteristics of their spermatozoa, the measurement of DNA integrity is one of the few markers which can be transferred to crustaceans to assess the quality of their semen. In the first step, the methods of collecting and maintaining spermatozoa were optimized. Cell survival was estimated during kinetics of preservation (i.e. 1, 2, 4 and 8 h) in various suspension media to define artificial seawater (ASW) as optimal. Several methods in the releasing of spermatozoa from the spermatophore of prawns were estimated with regard to their incidence both on the efficiency of extraction and the survival of cells. Pipetting up and down turned out to be the most successful and the least invasive technique. Secondly, the transfer of Comet assay was optimized by studying various times in both cell lysis (i.e. 1, 6, 18 h) and DNA denaturation (i.e. 15, 30 and 45 min), after in vitro exposure of spermatozoa to an H2O2 gradient as model genotoxicant. Results revealed that a minimum of 1 h in cell lysis and 15 min of DNA denaturation were sufficient to obtain valuable results, linked with a low compaction of DNA in spermatozoa of Palaemon sp. Finally, the sensitivity of P. serratus spermatozoa was assessed after in vitro exposures to model genotoxicants displaying various modes of interaction with DNA (i.e. UV-C, 13.3-79.5 J m; HO, 5-10 μM and MMS, 0.5-5 mM) and some environmental contaminants known or suspected to be genotoxic (i.e. cadmium and diuron, 0.015-1.5 μg L; carbamazepine, 0.1-10 μg L) for invertebrates. The low variability of the baseline level of DNA strand breaks recorded in controls highlighted the robustness of the method. P. serratus spermatozoa displayed significant DNA damage from the lowest doses tested for all model genotoxicants, but conversely, no genotoxic effect of tested environmental contaminants was observed. These results, which are discussed according to the protocol tested in the present study and the comparison with literature data, could suggest a difference in the response or sensitivity of spermatozoa to environmental genotoxicity between invertebrate species, and therefore the interest of Palaemonidae prawns in ecogenotoxicology. In conclusion, the present study underlines the potential of the Comet assay as a marker to assess the contamination impact on the sperm quality in Palaemonidae prawns in view to a potential application for in situ biomonitoring surveys.

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“…The microtubules within each arm are not arranged in the 9 + 2 axonemal pattern typical of the flagellated tails of spermatozoa of other animals. Therefore, they are not responsible for sperm movement (Tudge, 2009;Poljaroen et al, 2010). One hundred and fifty proteins from nine different categories have been identified in the signal crayfish P. leniusculus spermatophore, the most diverse categories in the protein profile being cytoskeleton proteins including actin and tubulin (Niksirat et al, 2014a).…”

Section: Biology Of Gametes In the Crayfishmentioning

confidence: 99%

Different aspects of reproduction strategies in crayfish: A review

Yazicioglu

Reynolds

Kozák

2016

Knowl. Manag. Aquat. Ecosyst.

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-Study of the reproductive strategy of crayfish species is of great importance in the current astacological world. Crayfish are among the largest freshwater invertebrates, and as keystone species, they are able to regulate the structure of the benthic fauna in the freshwaters, demonstrating different ecological strategies and life spans ranging up to 20+ years. In order to bring together the various pieces of information related to this issue, this overview of published scientific reports was conducted. The majority of crayfish species studied show sexual dimorphism, with approximately equal numbers of males and females. However, over some decades numerous observations have been made for a few species that may have different modes of reproduction, such as hermaphroditism or intersex (e.g. Cherax quadricarinatus, Samastacus spinifrons, Parastacus virilastacus and Pacifastacus leniusculus) and parthenogenesis (only Procambarus fallax f. virginalis).A recent study showed a new case of parthenogenesis as apomictic parthenogenesis (only Orconectes limosus). In addition, there are many investigations into the reproduction biology of crayfish, including using eyestalk ablation or androgenic gland ablation under various lab conditions and hybridization under natural conditions (e.g. Astacus astacus X Astacus leptodactylus, Orconectes rusticus X Orconectes propinquus). There are also some chemical factors which could possibly affect the reproduction system of crayfish in the wild.Key-words: crustacea / parthenogenesis / intersex / hybridization Résumé -Différents aspects des stratégies de reproduction des écrevisses : une revue. L'étude de la stratégie de reproduction des espèces d'écrevisses est d'une grande importance dans le monde astacicole actuel. Les écrevisses sont parmi les plus grands invertébrés d'eau douce, et comme des espèces clés, elles sont capables de réguler la structure de la faune benthique dans les eaux douces, présentant différentes stratégies écologiques et des durées de vie allant jusqu'à 20 ans. Afin de réunir les différents éléments d'information relatifs à cette question, une revue des publications scientifiques a été conduite. La majorité des espèces d'écrevisses étudiées montrent un dimorphisme sexuel, avec un nombre approximativement égal de mâles et de femelles. Cependant, depuis quelques décennies, de nombreuses observations ont été faites pour quelques espèces qui peuvent avoir différents modes de reproduction, tels que l'hermaphrodisme ou l'intersexe (par exemple Cherax quadricarinatus, Samastacus spinifrons, Parastacus virilastacus et Pacifastacus leniusculus) et la parthénogenèse (seulement Procambarus fallax f. virginalis). Une étude récente a montré un nouveau cas de parthénogenèse, une parthénogenèse apomictique (seulement Orconectes limosus). En outre, il y a beaucoup de recherches sur la biologie de la reproduction de l'écrevisse, y compris celle de l'ablation du pédoncule oculaire ou ablation de la glande androgène dans diverses conditions de laboratoire et de l'hybridati...

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Spermatogenesis and distinctive mature sperm in the giant freshwater prawn, Macrobrachium rosenbergii (De Man, 1879)

Cited by 46 publications

References 44 publications

Transition of basic protein during spermatogenesis of Fenneropenaeus chinensis (Osbeck, 1765)

Transition of basic protein during spermatogenesis of Fenneropenaeus chinensis (Osbeck, 1765)

Assessment of sperm quality in palaemonid prawns using Comet assay: methodological optimization

Different aspects of reproduction strategies in crayfish: A review

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