Sperm vacuoles cannot help to differentiate fertile men from infertile men with normal sperm parameter values

Gatimel, Nicolas; Léandri, Roger; Marino, Luise V.; Esquerré-Lamare, Camille; Parinaud, Jean

doi:10.1093/humrep/deu206

Cited by 9 publications

(19 citation statements)

References 50 publications

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“…Despite the correlation between age, sperm concentration and chromatin decondensation, DNA fragmentation or vacuoles in the sperm head were not related to age. The agerelated DNA fragmentation was found in several studies Gatimel et al, 2014;Liu et al, 2014); our data show an abnormal pattern as DNA fragmentation increased in men aged between 35 and 39 years and decreased in the older age men. We have no explanation for this finding, which may be a consequence of the effect of new lifestyle stressors, as previously mentioned.…”

Section: Discussionsupporting

confidence: 74%

“…The clinical significance of sperm morphology evaluation is, however, still a matter of debate. In fact, contrasting results have been reported by different laboratories worldwide (Menkveld, 2010(Menkveld, , 2013, mainly owing to different staining procedures (Gatimel et al, 2014;Henkel et al, 2008), implying that the simple examination with the optical microscope cannot provide accurate information on the morphological quality of the spermatozoon.…”

Section: Introductionmentioning

confidence: 97%

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Vacuoles in sperm head are not associated with head morphology, DNA damage and reproductive success

Fortunato¹,

Boni

Leo³

et al. 2016

Reproductive BioMedicine Online

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In this retrospective study of 873 men enrolled for assisted reproduction techniques, relationships between sperm quality parameters, motile sperm organelle morphology examination (MSOME), DNA damage and live birth rate were evaluated. The presence of vacuoles in the sperm heads was detected by MSOME. Either chromatin decondensation or DNA fragmentation was used to study DNA damage. Results show that age significantly affected some of the examined parameters. In particular, sperm concentration was positively correlated (R = 0.088; P = 0.01) and chromatin decondensation was negatively correlated (R = -0.102; P = 0.003) with age. Furthermore, live birth rate was significantly lower in men aged 40 years or older (P < 0.02) compared with the younger age groups. The presence of sperm head vacuoles was not associated with head morphology, main sperm quality parameters, DNA fragmentation and live birth rate. Considering sperm heads in relation to the shape (normal/abnormal) and vacuoles (presence/absence), no significant variations in the occurrence of vacuoles in either normal or abnormal heads were found. These data suggest that vacuoles are physiological features that do not alter sperm functionality, and it seems that MSOME is not necessary for increasing the success of assisted reproduction techniques.

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Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 97%

Vacuoles in sperm head are not associated with head morphology, DNA damage and reproductive success

Fortunato¹,

Boni

Leo³

et al. 2016

Reproductive BioMedicine Online

View full text Add to dashboard Cite

show abstract

“…Although the origin of these vacuoles raises many questions, some strong correlations have been established between the morphology of the spermatozoon, in particular the presence of large vacuoles, and its nuclear quality (degree of chromatin condensation and/or DNA integrity, chromosomal content) [8]. However, the high frequency of sperm head vacuoles in fertile men highlights the physiological nature of some of these vacuoles [9,10]. The observation of sperm head vacuoles is used to select spermatozoa before microinjection: intracytoplasmic morphologically selected sperm injection (IMSI).…”

Section: Introductionmentioning

confidence: 99%

Intracytoplasmic morphologically selected sperm injection (IMSI) does not improve outcome in patients with two successive IVF-ICSI failures

Gatimel

Parinaud

Léandri

2016

J Assist Reprod Genet

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Purpose Assessment of sperm morphology has been reconsidered since 2001 with the development of motile sperm organelle morphology examination (MSOME). This observation technique that combines high magnification microscopy and the Nomarski interference contrast makes it possible to select spermatozoa with as few vacuoles as possible before microinjection into the oocyte (intracytoplasmic morphologically selected sperm injection, IMSI). More than 10 years after the development of IMSI, the indications of the IMSI technique and its ability to increase pregnancy and/ or birthrates (compared with conventional ICSI) are still subject to debate. We aimed to better define the interest of IMSI in the third attempt. Methods We assessed the benefit of IMSI by carrying out a retrospective comparative study between IMSI and conventional ICSI during a third ART attempt. Two hundred sixteen couples with two previous ICSI failures were studied between February 2010 and June 2014. Results IMSI did not significantly improve the clinical outcomes compared with ICSI, either for implantation (12 vs 10 %), clinical pregnancy (23 vs 21 %), or live birth rates (20 vs 19 %).Conclusion This study provides supplementary arguments for not achieving IMSI procedure in the third attempt after two previous ICSI failures.

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“…Gatimel et al did not find any significant differences between the vacuolar parameters and aniline blue staining, SCSA and TUNEL assay. In addition, they reported that all of the vacuole parameters values were identical in fertile and infertile men (Gatimel et al, 2014). There are limited studies about the relation between the presence of vacuole and sperm aneuploidy.…”

Section: Imsi and Sperm Chromatinmentioning

confidence: 99%

Noninvasive imaging systems for gametes and embryo selection in IVF programs: a review

Omidi

Faramarzi

Agha‐Rahimi

et al. 2017

Journal of Microscopy

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Optimizing the efficiency of the in vitro fertilization procedure by improving pregnancy rates and reducing the risks of multiple pregnancies simultaneously are the primary goals of the current assisted reproductive technology program. With the move to single embryo transfers, the need for more cost-effective and noninvasive methods for embryo selection prior to transfer is paramount. These aims require advancement in a more acquire gametes/embryo testing and selection procedures using high-tech devices. Therefore, the aim of the present review is to evaluate the efficacy of noninvasive imaging systems in the current literatures, focusing on the potential clinical application in infertile patients undergoing assisted reproductive technology treatments. In this regards, three advanced imaging systems of motile sperm organelle morphology examination, polarization microscopy and time-lapse monitoring for the best selection of the gametes and preimplantation embryos are introduced in full.

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Sperm vacuoles cannot help to differentiate fertile men from infertile men with normal sperm parameter values

Abstract: The study was funded by a grant from Association pour la Recherche sur les Traitements de la Stérilité. There are no competing interests to declare.

Cited by 9 publications

References 50 publications

Vacuoles in sperm head are not associated with head morphology, DNA damage and reproductive success

Vacuoles in sperm head are not associated with head morphology, DNA damage and reproductive success

Intracytoplasmic morphologically selected sperm injection (IMSI) does not improve outcome in patients with two successive IVF-ICSI failures

Noninvasive imaging systems for gametes and embryo selection in IVF programs: a review

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