Sperm Subpopulations in Boar (Sus scrofa) and Gazelle (Gazella dama mhorr) Semen as Revealed by Pattern Analysis of Computer-Assisted Motility Assessments

Abáigar, Teresa; Holt, W. V.; Harrison, R. A. P.; Barrio, Gabriel del

doi:10.1095/biolreprod60.1.32

Cited by 196 publications

(180 citation statements)

References 23 publications

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“…As a result, the CASA-subjected study of motility can be included in the group of functional tests that can be considered as useful for in vivo predictive evaluation of an ejaculate (Quintero-Moreno et al 2004). In this sense, our mean values obtained in boar sperm motion parameters incubated in control conditions, noncapacitation medium (TBM) for 1 h at 398C (Table 2), are within the range obtained by others authors (Holt et al 1996, Abaigar et al 1999, Quintero-Moreno et al 2004, which supports the use of this experimental approach to study the possible involvement of the PI3-K intracellular pathway in the regulation of motility of boar spermatozoa.…”

Section: Discussionsupporting

confidence: 85%

“…Most of the studies in boar semen have used CASA systems just to make an objective measurement of motility (Abaigar et al 1999, Eriksson et al 2001, Peñ a et al 2003a. Although the technology to objectively measure this intrinsic ability of sperm cells has improved in recent years, the practical meaning of the changes in the motion parameters of the spermatozoa observed in our experiment is difficult to interpret, since few experiments have been addressed at determining the real meaning of the changes of sperm kinematics.…”

Section: Discussionmentioning

confidence: 99%

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Inhibition of phosphatidylinositol 3-kinase modifies boar sperm motion parameters

Aparicio¹,

Gil²,

García-Herreros³

et al. 2005

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Motility is the most widely used indicator of sperm quality. Besides modulation by the cAMP pathway little is known regarding the intracellular pathways that regulate boar sperm motility. Recently the role of phosphatidylinositol 3-kinase (PI3-K) in the regulation of human sperm motility has been described. Therefore, the aim of this study was to investigate the role of PI3-K in boar sperm kinematics by using the specific PI3-K inhibitor, LY294002. Boar sperm was incubated up to 1 h in non-capacitating medium in the presence or absence of the cAMP analog, 8Br-cAMP or the PI3-K inhibitor, LY294002 or both. Boar sperm incubated in capacitating medium was treated in the presence or absence of LY294002. First, we have clearly identified that PI3-K is present in whole lysates of boar spermatozoa. Inhibition of PI3-K significantly increased boar sperm straight-line velocity, circular velocity and average velocity without an effect on the percentage of progressively motile spermatozoa in both media. Inhibition of PI3-K induced the same effects on boar sperm velocities as activation of the cAMP/protein kinase A (PKA) pathway and treatment with the PI3-K inhibitor, LY294002 had neither summatory nor synergic effects on boar sperm motion parameters when treated simultaneously with the cAMP analog 8Br-cAMP. Our data suggest that PI3-K plays a negative role, regulating boar sperm motion parameters through a possible inhibition of the cAMP/PKA activating pathway, and since some Computer Aided Sperm Analysis (CASA)-derived parameters have been related to field fertility our results point to the possibility of modulating sperm motile quality by modifying the PI3-K cellular pathway.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Inhibition of phosphatidylinositol 3-kinase modifies boar sperm motion parameters

Aparicio¹,

Gil²,

García-Herreros³

et al. 2005

Reproduction

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“…However, the sperm kinetic parameters are the most-used features to identify sperm subpopulations in humans (Chantler et al 2004) and animals (Holt et al 1996, Abaigar et al 1999, 2001, Quintero-Moreno et al 2003, MartinezPastor et al 2005a, Nunez-Martinez et al 2006). …”

Section: Introductionmentioning

confidence: 99%

“…However, this amount of information has so far been underrated, and motility evaluation has been limited to the classical univariate statistical analysis, variable by variable. This restricted overview could diminish the whole information value or trigger erroneous conclusions (Abaigar et al 1999). The use of the mean value is the result of the simplistic assumption that spermatozoa parameters follow a normal distribution.…”

Section: Introductionmentioning

confidence: 99%

Artificial neural networks for the definition of kinetic subpopulations in electroejaculated and epididymal spermatozoa in the domestic cat

et al. 2012

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This study was designed for the identification of different sperm kinetic subpopulations in feline semen using artificial neural networks (ANNs) and for the evaluation of the effect of ejaculation on motility patterns of these subpopulations. Seven tomcats presented for routine orchiectomy were electroejaculated, and after 5 days, orchiectomized and epididymal tail sperms were collected. Sperm motility characteristics were evaluated using a computer-assisted sperm analyzer that provided individual kinetic characteristics of each spermatozoon. A total of 23 400 spermatozoa for electroejaculated and 9200 for epididymal tail samples were evaluated using a multivariate approach, comprising principal component analysis and ANN classification. The multivariate approach allowed the identification and characterization of three different and well-defined sperm subpopulations. There were significant differences before (epididymal tail spermatozoa) and after (electroejaculated sperm) ejaculation in sperm kinetic subpopulation characteristics. In both epididymal and ejaculated samples, the majority of subpopulation was characterized by high velocity and progressiveness; however, the electroejaculated samples showed significantly higher values, suggesting that the microenvironment of the epididymal tail could affect the sperm motility or, alternatively, seminal plasma could increase the kinetic characteristics of the spermatozoa, indicating that only after ejaculation, the spermatozoa express their motility potential. Nevertheless, further studies are required to clarify the functional significance of each kinetic subpopulation.

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“…Este tipo de analise não determina somente a porcentagem da motilidade, mas também quantifica característica especificas do movimento espermático, (GARNER, 1997;MALMGREN, 1997), podendo ainda determinar a presença e a cinética das sub-populações de espermatozóides, como a avaliação da integridade das células por meio de sondas fluorescentes (MORTIMER, 1997;ABAIGAR, et al, 1999;VERSTEGEN et al, 2002;QUINTERO MORENO et al, 2003). Desta forma, Moses et al (1994) relataram que o conjunto destes parâmetros fornece detalhes que possibilitariam melhor avaliação da qualidade do sêmen.…”

Section: I T E R a T U R A | 37unclassified

Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo

Gallego¹

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A verdade é que, quando sua mente estava completamente alheia, ele tinha o pensamento mais estranho que qualquer lunático no mundo jamais tivera, pois lhe parecia razoável e necessário, tanto por uma questão de honra quanto para servir a nação, se tornar um cavaleiro errante e viajar pelo mundo em sua armadura e seu cavalo para buscar aventuras e envolver-se em tudo que ele havia lido sobre como os cavaleiros errantes se envolviam, aproveitando as oportunidades, colocando-se em perigo e corrigindo todo tipo de mal.-Miguel de Cervantes, Don Quixote. DEDICO.A Dios.A mis Papas Ramiro y Alba Lucia, su amor es la base de todas mis logros, Dios me bendijo al poder tenerlos a mi lado.A mi hermanita María del Pilar, eres la otra faz de una obra de arte, tu alegría y emotividad me llenan de felicidad. No te imaginas como te admiro.A mis abuelitas Ruth y Mary, Las abuelitas más dulces y tiernas que puedan existir, siempre las llevo en mi corazón.A mis Abuelos Libardo y Jorge (In memoriam), Sus vidas son una inspiración para mi. Es un honor llevar el apellido Mejia Gallego. Los Extraño A mi novia Dunia, Porque nuestros sueños se hagan realidad. LOS AMO !!!!! Agradecimientos.A mis tias Angela, Nohora y Liliana: Siempre han creido en mis capacidades, ello me ha llenado de confianza para llegar a donde estoy ahora, de todas he aprendido cosas importantes en la vida.Las llevo y las llevare en mi corazón siempre.A mis tios

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Sperm Subpopulations in Boar (Sus scrofa) and Gazelle (Gazella dama mhorr) Semen as Revealed by Pattern Analysis of Computer-Assisted Motility Assessments

Cited by 196 publications

References 23 publications

Inhibition of phosphatidylinositol 3-kinase modifies boar sperm motion parameters

Inhibition of phosphatidylinositol 3-kinase modifies boar sperm motion parameters

Artificial neural networks for the definition of kinetic subpopulations in electroejaculated and epididymal spermatozoa in the domestic cat

Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo

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