Sperm shape abnormality and urine mutagenicity in mice treated with niclosamide

Vega, Sabrina; Guzman, Patricia; García, López; Espinosa, José; Nava, Carlos

doi:10.1016/0165-1218(88)90099-7

Cited by 83 publications

(44 citation statements)

References 7 publications

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“…The product used in the program was niclosamide, an ethanolamine salt of 2',5-dichloro-4'-nitrosalicylanilide, manufactured under the trade name Bayluscide  , whose efficacy had previously been established (Gonnert 1961). Application of this product caused biocidal action on non-target plants and animals (Andrews et al 1983), besides causing genotoxicity and carcinogenic effects (Vega et al 1988). The high cost (Pieri 1995), the possibility of recolonization of breeding grounds (Sarquis et al 1997(Sarquis et al , 1998, and the ecological toxicity of this product were limitations on its use as an official molluscide in government programs to control schistosomiasis.…”

Section: Physiological Changes In Biomphalaria Glabratamentioning

confidence: 99%

Physiological changes in Biomphalaria glabrata say, 1818 (Pulmonata: Planorbidae) caused by sub-lethal concentrations of the latex of Euphorbia splendens var. hislopii N.E.B (Euphorbiaceae)

Mello-Silva

Vasconcellos

Pinheiro

et al. 2006

Mem. Inst. Oswaldo Cruz

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Molluscides have been used as one of the strategies to control schistosomiasis. Many plant extracts with molluscidal effects have been tested, but the action of the latex of Euphorbia splendens var. hislopii is considered the most promising because it meets the recommendations of the World Health Organization (WHO). The objective of this study was to determine the lethal dose and identify the effects of the different doses of latex of E. splendens var.hislopii on the physiology of Biomphalaria glabrata submitted to treatment for 24 h. The concentrations of glucose, uric acid and total proteins in the hemolymph and of glycogen in the digestive gland and cephalopodal mass were determined. The LD 50 value was 1 mg/l. The highest escape index was found to be at a concentration of 0.6 mg/l. The results showed that the latex of E. splendens var. hislopii caused a sharp reduction in the reserves of glycogen in the digestive gland and elevation of the protein content in the hemolymph of B. glabrata. Key words: plant molluscide -Euphorbia splendens var. hislopii -Biomphalaria glabrataThe use of molluscides as one of the strategies to control schistosomiasis began in Brazil in 1976, with the creation of the Special Schistosomiasis Control Program by the National Health Foundation (Machado 1982). The product used in the program was niclosamide, an ethanolamine salt of 2',5-dichloro-4'-nitrosalicylanilide, manufactured under the trade name Bayluscide  , whose efficacy had previously been established (Gonnert 1961). Application of this product caused biocidal action on non-target plants and animals (Andrews et al. 1983), besides causing genotoxicity and carcinogenic effects (Vega et al. 1988). The high cost (Pieri 1995), the possibility of recolonization of breeding grounds (Sarquis et al. 1997(Sarquis et al. , 1998, and the ecological toxicity of this product were limitations on its use as an official molluscide in government programs to control schistosomiasis.At the same time, various plants were tested as natural molluscides. Jurberg et al. (1989) (Mendes et al. 1984). Of these, E. splendens stands out for its molluscidal activity in doses under 0.5 mg/l, eight times smaller than the lethal dose for fish (Vasconcellos & Schall 1986).Some questions have arisen in the selection of plant molluscides, such as: toxicity, availability, annual growth, adaptability to different local conditions, and location of molluscidal activity in parts of the plant that easily regenerate, such as the leaves. To be eligible for use, a product must be storable and remain viable for at least one year; be physically and chemically stable; have ethnobotanical value; and be easy to extract and apply, preferably in aqueous extracts (Kloos & McCullough 1982). Considering all these criteria, in 1998 the Oswaldo Cruz Institute (IOC, Fiocruz, RJ) obtained a biotechnology patent on a method to collect, extract and apply E. splendens var. hislopii latex as a molluscide (Vasconcellos 2000).In the published works on plants with molluscidal action and ...

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Section: Physiological Changes In Biomphalaria Glabratamentioning

confidence: 99%

Physiological changes in Biomphalaria glabrata say, 1818 (Pulmonata: Planorbidae) caused by sub-lethal concentrations of the latex of Euphorbia splendens var. hislopii N.E.B (Euphorbiaceae)

Mello-Silva

Vasconcellos

Pinheiro

et al. 2006

Mem. Inst. Oswaldo Cruz

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“…An aliquot was taken from this suspension in a leukocyte hemocytometer up to mark 0.5 and then diluted with PBS up to mark 11. The suspension was mixed well and charged into a Neuabauer's counting chamber and the spermatids were counted as per the standard procedure (Vega et al, 1988;Narayana et al, 2002Narayana et al, , 2005b. In each animal, 5 transversely cut stage XIV tubules were identified.…”

Section: Cytotoxicity Of Mp On Testismentioning

confidence: 99%

An Organophosphate Insecticide Methyl Parathion (O- O- Dimethyl O-4-Nitrophenyl Phosphorothioate) Induces Cytotoxic Damage and Tubular Atrophy in the Testis Despite Elevated Testosterone Level in the Rat

Narayana

Prashanthi²,

Arunkumar³

et al. 2006

J. Toxicol. Sci.

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-Methyl parathion (MP) is an organophosphate pesticide used in agriculture, although quite often illegally used indoors to contain insects. The present study was planned to investigate the effects of MP on rat testis. Adult male Wistar rats (13-14 weeks) were treated with MP as follows. Experiment 1-0, 1.75, 3.5 or 7 mg/kg i.p. for 5 days and sacrificed on Day 14; experiment 2 and 3-0, 0.5, or 1 mg/kg i.p. for 12 days, and sacrificed on Days 130 and 77, respectively; experiment 4-0, 0.75, or 1.5 mg/kg i.p. for 25 days, and sacrificed on Day 17; experiment 5-0 or 3.5 mg/kg po for 25 days, and sacrificed on Day 17, after the last exposure. MP decreased the body weight and the testis weight in experiments 4 and 5 (p<0.05-0.001) due to decreased food intake and tubular atrophy respectively. MP increased the intra-testicular testosterone level and decreased the LH level in experiments 4 and 5. The seminiferous epithelium showed sloughing of germ cells, vacuoles, focal necrosis, and formation of multinucleated giant cells, cellular degeneration (nuclear pyknosis, halo appearance and shrinkage of nuclei) and tubular atrophy, especially in experiment 4. The degree of testicular damage was higher in experiment 4>5>1>3>2 indicating more effect of prolonged i.p. treatment. Homogenization-resistant spermatid count was decreased in experiments 1, 4 and 5, and MP also decreased the tubular diameter, and epithelial height (p<0.05-0.001). Incidences of stage XIV tubules, number of meiotic figures and elongating spermatids were also decreased, whereas the incidence of tubules showing epithelial sloughing increased (p<0.05-0.001). We conclude that MP is a reproductive toxicant in male rats which causes significant testicular damage in the testis.

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“…Two thousand sperms per animal were analysed for abnormal shapes following the criteria described by Wyrobek et al (1983). Sperm count per epididymis, following the method of Vega et al (1988), was also determined.…”

Section: Sperm-shape Abnormality Assaymentioning

confidence: 99%

Assessment of the genotoxic risk of Punica granatum L. (Punicaceae) whole fruit extracts

Sánchez-Lamar¹,

Fonseca²,

Fuentes³

et al. 2008

Journal of Ethnopharmacology

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Sperm shape abnormality and urine mutagenicity in mice treated with niclosamide

Cited by 83 publications

References 7 publications

Physiological changes in Biomphalaria glabrata say, 1818 (Pulmonata: Planorbidae) caused by sub-lethal concentrations of the latex of Euphorbia splendens var. hislopii N.E.B (Euphorbiaceae)

Physiological changes in Biomphalaria glabrata say, 1818 (Pulmonata: Planorbidae) caused by sub-lethal concentrations of the latex of Euphorbia splendens var. hislopii N.E.B (Euphorbiaceae)

An Organophosphate Insecticide Methyl Parathion (O- O- Dimethyl O-4-Nitrophenyl Phosphorothioate) Induces Cytotoxic Damage and Tubular Atrophy in the Testis Despite Elevated Testosterone Level in the Rat

Assessment of the genotoxic risk of Punica granatum L. (Punicaceae) whole fruit extracts

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