Sperm Sexing Mediated by Magnetic Nanoparticles in Donkeys, a Preliminary In Vitro Study

Domïnguez, E.; Moreno-Irusta, Ayelén; Castex, Hernan Ramírez; Bragulat, Ana Flores; Ugaz, Cristian; Clemente, Henry; Giojalas, Laura Cecilia; Losinno, Luis

doi:10.1016/j.jevs.2018.04.005

Cited by 27 publications

(36 citation statements)

References 25 publications

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“…Sperm recovery was performed as described by Domínguez et al., (2018). The iron oxide MNP has a diameter of 50 nm coated with silica provided by Dr. NahidShahabadi (Faculty of Chemistry, Razi University, Kermanshah, Iran).…”

Section: Methodsmentioning

confidence: 99%

“…Sperm cells with the X chromosome and those with the Y chromosome have potentially Z values equal to −20 and −16 mV, respectively (Ishijima, Okuno, & Mohri, 1991). Therefore, Z potential and MNP interaction can be an effective alternative technique that is faster, easier and, cheaper than the flow cytometry or other methods to recover X chromosomes bearing sperms (Domínguez et al., 2018). Nanotechnology combines science, engineering and technology, involving a nanometre scale of 1–100 widely used in energy, medical drug and products as well as agricultural products (Odhiambo et al., 2014).…”

Section: Introductionmentioning

confidence: 99%

“…(Makker, Agarwal, & Sharma, 2008). The only study using MNP to separate sperms containing the X chromosome from those containing the Y chromosome was that performed by Domínguez et al., (2018) on donkey semen.…”

Section: Introductionmentioning

confidence: 99%

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Recovery of sperms bearing X chromosomes with different concentrations of magnetic nanoparticles in ram

Moradi

Hajarian

Karamishabankareh

et al. 2020

Reprod Domestic Animals

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Pre‐conceptual sex selection is still a highly debatable process whereby X and Y chromosome bearing spermatozoa are isolated before oocyte fertilization. Recently, magnetic nanoparticles (MNP) have been used to determine X and Y chromosomes bearing spermatozoa as a result of searching for a cheap, highly efficient method using non‐toxic materials. This study aimed to recover the sperm bearing X chromosomes in ram with different concentrations of MNP and then evaluate the success of this method using polymerase chain reaction (PCR). Ram sperms were divided into four groups, treated with 0 (control), 50, 100 and 200 μg/ml MNP, respectively. MNP was used to restore sperm cells bearing X chromosomes. Upon recovery, the PCR was performed to identify the X and Y sperms, Methyl ThiazoleTetrazolium (MTT), to assess MNP toxicity and sperm viability and acridine orange (AO) to evaluate sperm DNA integrity. The results of PCR revealed that the treatment of spermatozoa‐ bearing X chromosomes with 50 μg/ml MNP had the highest effects on the recovery of X sperm rather than the other concentrations of MNP. However, the concentrations of MNP did not have any toxic effects on spermatozoa, sperm viability and, DNA integrity, but the high concentration of MNP (200 μg/ml) significantly reduced DNA integrity. According to MTT and AO results, the concentrations of MNP used in this study had no toxic effects on spermatozoa and did not reduce the sperm viability and DNA integrity, except that 200 μg/ml MNP significantly reduced DNA integrity.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Recovery of sperms bearing X chromosomes with different concentrations of magnetic nanoparticles in ram

Moradi

Hajarian

Karamishabankareh

et al. 2020

Reprod Domestic Animals

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show abstract

“…In the build-up phase of a donkey farming system, having more female offspring is desirable. Sperm sexing technology exists and has been applied to and assessed for dairy and beef cattle but more recently also to polo horses [58,59]. In a recent study on sperm sexing in donkeys, Domínguez et al [59] report that after the sexing procedure, 90 percent of sperm has the X chromosome.…”

Section: The Donkey Farming System Modelmentioning

confidence: 99%

“…Sperm sexing technology exists and has been applied to and assessed for dairy and beef cattle but more recently also to polo horses [58,59]. In a recent study on sperm sexing in donkeys, Domínguez et al [59] report that after the sexing procedure, 90 percent of sperm has the X chromosome. Sexing sperm can negatively impact conception rates although the new sexing technique had no impact on conception rates in horses.…”

Section: The Donkey Farming System Modelmentioning

confidence: 99%

The Potential for New Donkey Farming Systems to Supply the Growing Demand for Hides

Bennett

Pfuderer

2020

Animals

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The demand for donkey hides for ejiao, a Traditional Chinese Medicine, has resulted in rapidly increasing prices for donkey hides and donkeys. This has put pressure on donkey populations globally and has implications for donkey welfare and the livelihoods of those who rely on donkeys as working animals. The aim of the research was to explore the feasibility of setting up new donkey farming systems to supply the rising demand for ejiao using a system dynamics model of donkey production. Results show that the size of the initial female breeding herd, reproductive performance, age of reproduction, percentage of female births and average breeding life of donkeys are key variables affecting the time to build up the donkey population to supply the demand for hides, which will be at least ten to fifteen years. The implications of this are: (i) prices for donkey hides will continue to increase, (ii) companies producing ejiao will use other ingredients, (iii) China will continue to source donkey hides from around the world, and (iv) there will be continued theft and illegal trade of donkeys and concerns for rural households reliant on donkeys for their livelihoods and adverse impacts on donkey welfare.

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Review on Sperm Sorting Technologies and Sperm Properties toward New Separation Methods via the Interface of Biochemistry and Material Science

et al. 2019

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Successful fertilization in mammals requires spermatozoa to efficiently traverse the female reproductive tract to meet the egg. This process naturally selects high quality sperm cells for fertilization, but when artificial reproductive technologies are used such as in vitro fertilization, intracytoplasmic sperm injection, or intrauterine insemination, other methods of sperm selection are required. Currently, technology enables sperm sorting based on motility, maturity as defined by zeta potential or hyaluronic acid binding site expression, absence of apoptotic factors, appropriate morphology, and even sex. This review summarizes current knowledge on all known methods of sperm cell sorting, compares their efficiency, and discusses the advantages and limitations of each technique. Scope for further refinement and improvement of current methods are discussed as is the potential to utilize a variety of materials to innovate new methods of sperm separation.

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Sperm Sexing Mediated by Magnetic Nanoparticles in Donkeys, a Preliminary In Vitro Study

Cited by 27 publications

References 25 publications

Recovery of sperms bearing X chromosomes with different concentrations of magnetic nanoparticles in ram

Recovery of sperms bearing X chromosomes with different concentrations of magnetic nanoparticles in ram

The Potential for New Donkey Farming Systems to Supply the Growing Demand for Hides

Review on Sperm Sorting Technologies and Sperm Properties toward New Separation Methods via the Interface of Biochemistry and Material Science

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