Sperm RNA elements as markers of health

Burl, Rayanne B.; Clough, Stephanie; Sendler, Edward; Estill, Molly; Krawetz, Stephen A.

doi:10.1080/19396368.2017.1393583

Cited by 33 publications

(19 citation statements)

References 79 publications

(115 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have previously identified sperm RNAs required for live birth [30]. Gene ontology along with developmental and disease associations of sperm RNAs and DNA methylation analyses suggest a potential impact on child health and development [29,31,32]. We sought to define the relationship between obesity as measured by BMI and human sperm RNAs to expand our understanding garnered from animal models of obesity [33,34] and other human studies [4,29,35].…”

Section: Introductionmentioning

confidence: 99%

Human chromatin remodeler cofactor, RNA interactor, eraser and writer sperm RNAs responding to obesity

et al. 2019

Self Cite

View full text Add to dashboard Cite

In the United States almost 33% of adults are considered obese (BMI > 30 kg/m 2). Both animal models and to a lesser extent human studies, have associated BMI, a measure of obesity, with alterations in sperm DNA methylation and RNAs. Sperm RNAs from the Assessment of Multiple Gestations from Ovarian Stimulation trial, were isolated and sequenced. A Generalized Linear Model identified 487 BMI associated human sperm RNA elements (short exon-sized sequences). They partitioned into four patterns; a continual increase with BMI, increase once obese (BMI>30 kg/m 2); a steady decrease with BMI; and decrease once overweight (BMI 25-30 kg/ m 2). Gene Ontology revealed a unique relationship between BMI and transcripts associated with chromosome organization, adipogenesis, cellular stress and obesity-related inflammation. Coregulatory networks linked by Chromatin remodeler cofactors, RNA interactors, Erasers and Writers (CREWs) were uncovered to reveal a hierarchical epigenetic response pathway.

show abstract

Section: Introductionmentioning

confidence: 99%

Human chromatin remodeler cofactor, RNA interactor, eraser and writer sperm RNAs responding to obesity

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…In mammals, it has been well established that Ndrg1 exerts an inhibitory effect on cancer cell proliferation (Chang et al, 2014;McCaig et al, 2011;Xi et al, 2017;Zhang et al, 2019). Additionally, several studies have shown that Ndrg1 is expressed in the gonads of humans, mice, sheep and medaka (Burl et al, 2018;Kikuchi et al, 2019;Lachat et al, 2002;Qu et al, 2019;Stévant and Nef, 2019), however its role in gonadal function and reproduction is still unknown. In medaka, there are two paralogs of ndrg1: ndrg1a and ndrg1b, although only ndrg1b is expressed in the gonad.…”

Section: Discussionmentioning

confidence: 99%

Cystic proliferation of embryonic germ stem cells is necessary to reproductive success and normal mating behavior in medaka

Padilla

Castañeda-Cortés

Rosa

et al. 2020

Preprint

View full text Add to dashboard Cite

The production of an adequate number of gametes in both sexes is necessary for normal reproduction, for which the regulation of proliferation from early gonadal development to adulthood is key. Cystic proliferation of embryonic stem germ cells prior the onset of gametogenesis is an especially important step prior to the beginning of meiosis. However, in vertebrates, the molecular regulators of cystic proliferation remain unknown. Here, we report that ndrg1b, a member of the N-myc downstream regulated family, is an important regulator of cystic proliferation in medaka. We generated mutants of ndrg1b that led to a disruption of proliferation type II, independently of the TGF-β signaling pathway. This loss of cystic proliferation was observed from embryogenic to adult stages, impacting the success of gamete production and reproductive parameters such as spawning and fertilization. Interestingly, the depletion of cystic proliferation of the ndrg1b mutant also impacted male sexual behavior, with a decrease of mating vigor. These data illustrate why it is also necessary to consider gamete production capacity in order to analyze reproductive behavior.HIGHLIGHTSNdrg1b is involved in the regulation of cystic proliferation in gonad from embryo to adulthood.The cystic proliferation is independently of the TGF-β signaling pathway.Decrease of production of gametes declines reproductive success for both sexes.Reduction of cystic proliferation declines male sexual behavior, with a decrease of mating vigor.

show abstract

“…There are no treatments currently available for defects in the RNAs that are reflective of disease, but it does provide a mechanistic understanding of the basis of infertility [77][78][79]. RNA content may be a predictor of human health and could be used to monitor the impact and recovery from environmental exposures [80,81]. As a result, assessing sperm RNA has the potential to evaluate the father's contribution, his current health status, and the health of his future child [80].…”

Section: Rna-sperm Transcriptsmentioning

confidence: 99%

“…RNA content may be a predictor of human health and could be used to monitor the impact and recovery from environmental exposures [80,81]. As a result, assessing sperm RNA has the potential to evaluate the father's contribution, his current health status, and the health of his future child [80].…”

Section: Rna-sperm Transcriptsmentioning

confidence: 99%

Male Infertility is a Women’s Health Issue—Research and Clinical Evaluation of Male Infertility Is Needed

Turner

Rambhatla

Schon

et al. 2020

Cells

Self Cite

View full text Add to dashboard Cite

Infertility is a devastating experience for both partners as they try to conceive. Historically, when a couple could not conceive, the woman has carried the stigma of infertility; however, men and women are just as likely to contribute to the couple's infertility. With the development of assisted reproductive technology (ART), the treatment burden for male and unexplained infertility has fallen mainly on women. Equalizing this burden requires reviving research on male infertility to both improve treatment options and enable natural conception. Despite many scientific efforts, infertility in men due to sperm dysfunction is mainly diagnosed by a semen analysis. The semen analysis is limited as it only examines general sperm properties such as concentration, motility, and morphology. A diagnosis of male infertility rarely includes an assessment of internal sperm components such as DNA, which is well documented to have an impact on infertility, or other components such as RNA and centrioles, which are beginning to be adopted. Assessment of these components is not typically included in current diagnostic testing because available treatments are limited. Recent research has expanded our understanding of sperm biology and suggests that these components may also contribute to the failure to achieve pregnancy. Understanding the sperm's internal components, and how they contribute to male infertility, would provide avenues for new therapies that are based on treating men directly for male infertility, which may enable less invasive treatments and even natural conception.

show abstract

Sperm RNA elements as markers of health

Cited by 33 publications

References 79 publications

Human chromatin remodeler cofactor, RNA interactor, eraser and writer sperm RNAs responding to obesity

Human chromatin remodeler cofactor, RNA interactor, eraser and writer sperm RNAs responding to obesity

Cystic proliferation of embryonic germ stem cells is necessary to reproductive success and normal mating behavior in medaka

Male Infertility is a Women’s Health Issue—Research and Clinical Evaluation of Male Infertility Is Needed

Contact Info

Product

Resources

About