Sperm Release From the Oviductal Epithelium Depends on Ca<sup>2+</sup>Influx Upon Activation of CB1 and TRPV1 by Anandamide

Gervasi, María Gracia; Osycka‐Salut, Claudia; Sánchez, Trinidad; Alonso, C.; Llados, Carmen; Castellano, Luciana; Franchi, A.M.; Villalón, Manuel; Pérez‐Martínez, Silvina

doi:10.1002/jcb.25273

Cited by 21 publications

(22 citation statements)

References 51 publications

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“…One report demonstrated that during peri-ovulatory period AEA induce bull sperm capacitation by activate CB1 and TRPV1 receptors, with the help of heparin, suggest effects of AEA may differ in different species [211]. AEA also regulates the sperm release from oviductal epithelia with the participation of NO · [212] and calcium [213]. Another report showed that the effects of AEA on human sperm motility were dependent on the reduction of sperm mitochondrial activity, and that it also inhibits capacitation-induced acrosome reaction [214].…”

Section: Different Pathways In Capacitationmentioning

confidence: 99%

Factors and pathways involved in capacitation: how are they regulated?

Jin

Yang

2016

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In mammals, fertilization occurs via a comprehensive progression of events. Freshly ejaculated sperm have yet to acquire progressive motility or fertilization ability. They must first undergo a series of biochemical and physiological changes, collectively known as capacitation. Capacitation is a significant prerequisite to fertilization. During the process of capacitation, changes in membrane properties, intracellular ion concentration and the activities of enzymes, together with other protein modifications, induce multiple signaling events and pathways in defined media in vitro or in the female reproductive tract in vivo. These, in turn, stimulate the acrosome reaction and prepare spermatozoa for penetration of the egg zona pellucida prior to fertilization. In the present review, we conclude all mainstream factors and pathways regulate capacitation and highlight their crosstalk. We also summarize the relationship between capacitation and assisted reproductive technology or human disease. In the end, we sum up the open questions and future avenues in this field.

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Section: Different Pathways In Capacitationmentioning

confidence: 99%

Factors and pathways involved in capacitation: how are they regulated?

Jin

Yang

2016

Oncotarget

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“…In our laboratory, we have developed an in vitro model to study the sperm-oviduct interaction in cattle [Gervasi et al, 2009[Gervasi et al, , 2015. In our laboratory, we have developed an in vitro model to study the sperm-oviduct interaction in cattle [Gervasi et al, 2009[Gervasi et al, , 2015.…”

Section: Discussionmentioning

confidence: 99%

“…These studies were performed using an in vitro model to evaluate the sperm-oviduct interaction developed previously in our laboratory [Gervasi et al, 2009[Gervasi et al, , 2015Osycka-Salut et al, 2012]. The presence of Fn in the apical region of epithelial cells suggests a possible participation of Fn in the binding to spermatozoa during the formation of the sperm reservoir.…”

Section: Participation Of Fn In the Sperm-oviduct Interactionmentioning

confidence: 99%

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Fibronectin From Oviductal Cells Fluctuates During the Estrous Cycle and Contributes to Sperm–Oviduct Interaction in Cattle

Osycka‐Salut

Castellano

Fornes

et al. 2017

J of Cellular Biochemistry

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During the passage of sperm through the oviduct, spermatozoa bind to the oviductal epithelium and form the oviductal reservoir. This interaction keeps the fertilizing capacity of sperm until ovulation-associated signals induce sperm release from the oviductal epithelium, allowing the transit of spermatozoa to the fertilization site. Fibronectin is a glycoprotein from the extracellular matrix that binds to α5β1 receptors. Fibronectin has been found to be expressed in the oviduct, whereas α5β1 has been found to be expressed in the sperm of different species. Fibronectin is involved through α5β1 in sperm functions. The aim of this work was to study the participation of oviductal fibronectin in the regulation of the sperm-oviduct interaction in cattle. We found that oviductal epithelial cells differentially expressed all mRNA splice variants of fibronectin during the estrous cycle. Fibronectin was localized in the apical region of oviductal epithelial cells and fibronectin levels in the oviductal fluid fluctuated during the estrous cycle. Also, bovine spermatozoa expressed α5β1. Using in vitro sperm-oviduct co-cultures, we found that spermatozoa were attached to the oviductal epithelium through α5β1. The incubation of co-cultures with fibronectin induced sperm release from the oviductal cells through α5β1. The sperm population released from oviductal cells by fibronectin was enriched in motile and capacitated spermatozoa. Based on our in vitro culture system results, we propose that fibronectin and α5β1 are involved in the sperm-oviduct interaction. Also, an increase in fibronectin levels in the oviductal fluid during the pre-ovulatory period may promote sperm release from the oviductal epithelium in cattle. J. Cell. Biochem. 118: 4095-4108, 2017. © 2017 Wiley Periodicals, Inc.

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“…It has been reported that anandamide (AEA), an endocannabinoid synthesized by sperm and oviductal cells, activates TRPV1 and the ligand-receptor interaction occurs through a cytosolic binding site triggering an increase of intracellular Ca + concentration and/or activation of protein kinases (20). TRPV1 is expressed in mammalian spermatozoa and its activation is involved in the regulation of sperm oviduct interaction, sperm capacitation and acrosome reaction (21)(22)(23). On the other hand, sperm release from the oviductal reservoir depends on Ca + influx by activation of cannabinoid type migration toward a temperature gradient that was reduced by the addition of selective antagonists of TRPV1.…”

mentioning

confidence: 99%

Mechanisms of the sperm guidance, an essential aid for meeting the oocyte

Lottero-Leconte¹,

Alonso²,

Castellano³

et al. 2017

Transl. Cancer Res

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In mammals, ejaculated spermatozoa must migrate into the female reproductive tract in order to reach and fertilize the oocyte (Figure 1). The number of spermatozoa that reach the oviductal isthmus (where they attach to oviductal cells and form the sperm reservoir) is small (1,2) and only ~10% of these spermatozoa in humans become capacitated (3) and acquire a state of readiness for fertilizing the oocyte. In addition, the sperm cells have a torturous and long way between the reservoir and the oocyte at the fertilization site (3-5 cm in humans) (2). These facts, together with the tiny dimensions of the gametes in comparison to the tube length make improbable the sperm arrival to the fertilization site and make evident the need for sperm guidance (4). Different guidance mechanisms appear essential for successful sperm arrival to the fertilization site: the short range mechanism such as chemotaxis (swimming up a chemoattractant gradient) (5) and the long range mechanisms such as rheotaxis (swimming against a fluid flow) and thermotaxis (the temperature-oriented cell motility) (Figure 1).When spermatozoa become capacitated, they acquire a number of properties that render them fertilizing ability. Thermotaxis is one of these properties in which sperm cells acquire the ability to be thermotactically active (6). This property is manifested by the ability of the capacitated spermatozoa to change their swimming direction according to a temperature gradient (towards the warmer temperature) (Figure 1). Spermatozoa can sense both the absolute ambient temperature and the temperature gradient. Previous studies showed that, at peri-ovulation stage, there is a temperature difference between the sperm reservoir site (cooler) and the fertilization site (warmer). Thus, a temperature difference of ~2 ℃ exists in rabbits between the isthmus and the isthmicampullary junction (7) and ~0.7 ℃ difference in mated pigs between the isthmus and the ampulla (8). Subsequently, Bahat and collaborators (9) demonstrated that rabbit and human spermatozoa can sense small temperature differences and respond to it by thermotaxis. These findings raised the hypothesis that the temperature difference might serve as a cue for guiding spermatozoa to the site of fertilization. Interestingly, Bahat et al.,(10) recently has reported that human spermatozoa are thermotactically responsive over a wide temperature range (29-41 ℃), much wider than the range considered physiological.As in sperm chemotaxis, only capacitated spermatozoa are thermotactically responsive (9). Additionally, in chemotaxis and thermotaxis, spermatozoa swim up the gradient by modulating the frequency of turns and hyperactivation events, essentially in the direction of the gradient (11,12).The molecular bases of sperm thermotaxis remain poorly understood. Taking into account that thermotaxis favours Editorial

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Sperm Release From the Oviductal Epithelium Depends on Ca²⁺Influx Upon Activation of CB1 and TRPV1 by Anandamide

Cited by 21 publications

References 51 publications

Factors and pathways involved in capacitation: how are they regulated?

Factors and pathways involved in capacitation: how are they regulated?

Fibronectin From Oviductal Cells Fluctuates During the Estrous Cycle and Contributes to Sperm–Oviduct Interaction in Cattle

Mechanisms of the sperm guidance, an essential aid for meeting the oocyte

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Sperm Release From the Oviductal Epithelium Depends on Ca2+Influx Upon Activation of CB1 and TRPV1 by Anandamide

Cited by 21 publications

References 51 publications

Factors and pathways involved in capacitation: how are they regulated?

Factors and pathways involved in capacitation: how are they regulated?

Fibronectin From Oviductal Cells Fluctuates During the Estrous Cycle and Contributes to Sperm–Oviduct Interaction in Cattle

Mechanisms of the sperm guidance, an essential aid for meeting the oocyte

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Sperm Release From the Oviductal Epithelium Depends on Ca²⁺Influx Upon Activation of CB1 and TRPV1 by Anandamide