Sperm quality of Brazilian flounder Paralichthys orbignyanus throughout the reproductive season

Lanes, Carlos Frederico Ceccon; Okamoto, Marcelo Hideo; Bianchini, Adalto; Marins, Luís Fernando; Sampaio, Luís André

doi:10.1111/j.1365-2109.2010.02501.x

Cited by 27 publications

(24 citation statements)

References 41 publications

(60 reference statements)

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“…sperm density between different species of the same genus may be caused by differences in the geographic range or by sampling at different periods during the spawning season (Lanes et al 2010). The best result among the different ionic compositions and pH values tested in this study was achieved in the extender solution at 172 mOsm and pH 8.2.…”

Section: -1 (Vuthiphandchai Et Al 2009) the Variation Inmentioning

confidence: 53%

Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Sanches

Oliveira

Serralheiro

et al. 2013

An. Acad. Bras. Ciênc.

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This study aimed to develop a protocol of semen cryopreservation of the mutton snapper Lutjanus analis. The interaction between three extenders (pH 6.1; 7.8 and 8.2), two concentrations of dimethyl sulfoxide (dMSO, 5 and 10%) and three cooling rates (-90; -60 and -30°C.min ) on the sperm motility rate and motility time were analyzed by a factorial experiment. A sample of 30 fishes (1,261 ± 449 g) collected in the nature was kept in floating net cages. The semen was frozen by using cryogenic straws, in nitrogen vapour and transferred, later, to liquid nitrogen. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The highest sperm motility rate and motility time (P < 0.05) was achieved by combining extender C (ph 8.2) with dMSO (10%) and cooling rate of -60°C.min -1 (P < 0.05). The use of cryopreserved sperm presented fertilization rates higher than 59% validating the present protocol for mutton snapper.

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Section: -1 (Vuthiphandchai Et Al 2009) the Variation Inmentioning

confidence: 53%

Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Sanches

Oliveira

Serralheiro

et al. 2013

An. Acad. Bras. Ciênc.

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“…For lane snapper the Colombian coast, sperm density reported was 1.3 × 10 9 cells mL -1 (Gaitán-Espitia et al, 2013). The variation in sperm density among different species of the same genus may be caused by differences in the geographic range or by sampling at different periods during the spawning season (Lanes et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

et al. 2015

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This study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C -min ), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C Criopreservação do sêmen do ariocó Lutjanus synagris (Linnaeus, 1758) ResumoEste estudo teve a finalidade de desenvolver e avaliar um protocolo de crioconservação do sêmen do ariocó Lutjanus synagris. Para caracterizar o sêmen foram avaliados a taxa de motilidade, a duração da motilidade, a concentração espermática e o espermatócrito. Em seis experimentos foram analisados os efeitos de três diluentes, com distintas composições iônicas e valores de pH distintos, combinados com sete concentrações de dimetilsulfóxido (0; 2,5; 5,0; 7,5; 10,0; 12,5 e 15,0%), cinco velocidades de congelamento (-110, -90, -60, -45 e -30°C/min), nove tempos de equilíbrio (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutos) e cinco proporções de sêmen:diluente (1:1; 1:3; 1:6; 1:10 e 1:20) sobre a taxa de motilidade e a duração da motilidade espermáticas. Posteriormente um teste de fertilização foi realizado para avaliar a viabilidade do sêmen crioconservado. O tratamento que propiciou maior taxa de motilidade e duração da motilidade espermáticas (P<0,05) foi aquele proporcionado pelo emprego do diluente com pH 8,2 com dimetilsulfóxido a 10%, em uma velocidade de congelamento de -60°C/min, com tempo de equilíbrio de 1 minuto e na proporção de 1:3 (v/v). O sêmen crioconservado apresentou taxa de fertilização superior a 69% validando o presente protocolo para o ariocó. A crioconservação do sêmen do ariocó é uma alternativa viável, sendo possível manter uma apropriada qualidade espermática.

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“…1995;RIVERA et al, 2001;NORBIS;GALLI, 2004) and in the mouths of rivers and streams (PLAVAN et al, 2010). This ability to live in estuarine environments together with its wide tolerance to environmental factors such as salinity and pH (WASIELESKY et al, 1997;SAMPAIO et al, 2001SAMPAIO et al, , 2002 and its high market price, make P. orbignyanus an interesting candidate for aquaculture (ROBALDO, 2003;BAMBILL et al, 2006;RADONIC et al, 2007;SAMPAIO et al, 2007;MACCHI, 2009;LANES et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…The spawning season of P. orbignyanus extends from October to April (SILVEIRA et al, 1995;ROBALDO, 2003), with a peak of reproductive activity between January and April (LANES et al, 2010), while that of P. patagonicus lasts from September to February with a peak of maximum reproductive activity in November (MACHI; . During the rest of the year outside the breeding season it is usual to find P. orbignyanus feeding in brackish waters, coastal lagoons or rivers, whereas P. patagonicus spends the resting phase feeding in the marine environment.…”

Section: Introductionmentioning

confidence: 99%

Life history traits influence in gonad composition of two sympatric species of flatfish

et al. 2015

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Life history traits influence in gonad composition of two sympatric species of flatfishParalichthys orbignyanus and Paralichthys patagonicus are flatfish with different life history traits, having in common the condition of breeding in seawater. Paralichthys patagonicus remain their whole life in open seawater and Paralichthys orbignyanus are sometimes found in brackish water bodies. As marine and estuarine food webs have different fatty acid (FA) compositions, the aim of this study was to characterize the gonadal maturation of P. orbignyanus and P. patagonicus females through the analysis of lipid content and FA profile in order to understand to what extent life history traits are reflected in the ovarian composition. During gonadal maturation lipid content increased and FA profiles changed in both species, but the lipid increase was greater in P. orbignyanus. The N-3FA and n-3HUFA proportions increased in both species but were higher in P. orbignyanus. The differences between the lifestyles of these species were reflected in the ovarian FA profile mainly as a result of differences in their FA metabolism, causing a greater accumulation of n-3FA and n-3HUFA in P. orbignyanus than in P. patagonicus. The higher lipid accumulation in P. orbignyanus' ovaries could indicate that this species, feeding in brackish water bodies, has the possibility of storing more energy than P. patagonicus. Paralichthys patagonicus e Paralichthys orbignyanus apresentam diferentes atributos nas suas histórias de vida, embora ambas se reproduzam no mar. Paralichthys patagonicus permanece toda a vida em águas abertas, enquanto P. orbignyanus é comumente encontrado em corpos de água salobra. Considerando que redes alimentares marinhas e estuarinas têm diferente composição de ácidos graxos (FA), o objetivo deste estudo foi caracterizar a maturação gonadal de fêmeas de P. orbignyanus e P. patagonicus analisando o conteúdo lipídico e perfil FA, a fim de estabelecer se as diferenças nas histórias de vida são refletidas na composição do ovário. Durante a maturação gonadal, em ambas as espécies, houve aumento do teor lipídico e o perfil FA mudou, mas o incremento foi maior em P. orbignyanus. As proporções de N-3FA e n-3HUFA aumentaram em ambas as espécies, mas foram maiores em P. orbignyanus. As diferenças entre os estilos de vida destas espécies foram refletidas no perfil de FA dos ovários, principalmente como resultado das diferenças no metabolismo de FA, causando maior acúmulo de n-3FA e n-3HUFA em P. orbignyanus. O maior acúmulo de lipídios nos ovários de P. orbignyanus poderia indicar que esta espécie, ao se alimentar em águas salobras, tem a possibilidade de armazenar mais energia do que P. patagonicus. AbstrAct

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Sperm quality of Brazilian flounder Paralichthys orbignyanus throughout the reproductive season

Cited by 27 publications

References 41 publications

Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

Life history traits influence in gonad composition of two sympatric species of flatfish

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