Sperm quality may adversely affect the chromosome constitution of embryos that result from intracytoplasmic sperm injection

Obasaju, Michael Femi; Kadam, Arjun L.; Sultan, Khalid M.; Fateh, Majid; Munné, Santiago

doi:10.1016/s0015-0282(99)00391-x

Cited by 35 publications

(20 citation statements)

References 5 publications

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“…Furthermore, if a pregnancy was achieved, the fetus was aborted (107). Sperm harvested from the testicles before maturation may not have a fully functional centrosome, which could lead to problems with the segregation of chromosomes and result in a mosaic or aneuploid embryo (108,109). Abnormal centrosomes may also be related to the failure of the gametes to unite properly, another error that may cause cleavage arrest (107).…”

Section: Epigenetic Errorsmentioning

confidence: 99%

The genetic causes of male factor infertility: A review

O’Brien

Varghese²,

Agarwal

2010

Fertility and Sterility

400

258

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Section: Epigenetic Errorsmentioning

confidence: 99%

The genetic causes of male factor infertility: A review

O’Brien

Varghese²,

Agarwal

2010

Fertility and Sterility

400

258

View full text Add to dashboard Cite

Section: Contributions To Chromosomal Abnormality-paternal Factorsmentioning

confidence: 99%

“…Indeed Obasaju et al 1999 showed high rates of chaotic mosaics in patients with partners that had male factor infertility. This incident disappeared in the following cycle after patients used sperm donation [54]. Another recent hypothesis by Leduc et al 2008 suggested that alterations in the steps of chromatin remodeling or the DNA repair mechanism in elongating sperm during spermiogenesis are vulnerable to DNA fragmentation and continue to persist because spermatids lack a repair mechanism, which ultimately leads to infertility [55].…”

Section: Contributions To Chromosomal Abnormality-paternal Factorsmentioning

confidence: 99%

Preimplantation genetic screening: does it help or hinder IVF treatment and what is the role of the embryo?

Agarwal

Nagy

2011

J Assist Reprod Genet

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Despite an ongoing debate over its efficacy, preimplantation genetic screening (PGS) is increasingly being used to detect numerical chromosomal abnormalities in embryos to improve implantation rates after IVF. The main indications for the use of PGS in IVF treatments include advanced maternal age, repeated implantation failure, and recurrent pregnancy loss. The success of PGS is highly dependent on technical competence, embryo culture quality, and the presence of mosaicism in preimplantation embryos. Today, cleavage stage biopsy is the most commonly used method for screening preimplantation embryos for aneuploidy. However, blastocyst biopsy is rapidly becoming the more preferred method due to a decreased likelihood of mosaicism and an increase in the amount of DNA available for testing. Instead of using 9 to 12 chromosome FISH, a 24 chromosome detection by aCGH or SNP microarray will be used. Thus, it is advised that before attempting to perform PGS and expecting any benefit, extended embryo culture towards day 5/6 should be established and proven and the clinical staff should demonstrate competence with routine competency assessments. A properly designed randomized control trial is needed to test the potential benefits of these new developments.

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“…In addition, Greco et al in 2005 reported a clinical pregnancy rate (PR) of 0 % with microinjection of testicular sperm in patients with DNA fragmentation levels in semen >15 %, as measured by TdTmediated-dUTP nick-end labeling (TUNEL) [19]. Higher percentage of fragmented chromatin in a seminal sample is associated with a reduced pregnancy and implantation rates after conventional IVF and it is predictive of failure of embryo development in couples undergoing ICSI cycles [17] confirming the "paternal effect" of sperm DNA on the embryo development, as demonstrated by several other authors [10,41,61].…”

Section: Introductionmentioning

confidence: 64%

Lower sperm DNA fragmentation after r-FSH administration in functional hypogonadotropic hypogonadism

Ruvolo

Roccheri

Brucculeri

et al. 2013

J Assist Reprod Genet

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Purpose An observational clinical and molecular study was designed to evaluate the effects of the administration of recombinant human FSH on sperm DNA fragmentation in men with a non-classical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia. Methods In the study were included 53 men with a nonclassical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia. In all patients, sperm DNA fragmentation index (DFI), assessed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) in situ DNA nick end-labelling (TUNEL) assay, was evaluated before starting the treatment with 150 IU of recombinant human FSH, given three times a week for at least 3 months. Patients' semen analysis and DNA fragmentation index were re-evaluated after the 3-month treatment period. Results After recombinant human FSH therapy, we did not find any differences in terms of sperm count, motility and morphology. The average DNA fragmentation index was significantly reduced (21.15 vs 15.2, p<0.05), but we found a significant reduction in patients with high basal DFI values (>15 %), while no significant variation occurred in the patients with DFI values ≤15 %. Conclusions Recombinant human FSH administration improves sperm DNA integrity in hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia men with DNA fragmentation index value >15 % .

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Sperm quality may adversely affect the chromosome constitution of embryos that result from intracytoplasmic sperm injection

Cited by 35 publications

References 5 publications

The genetic causes of male factor infertility: A review

The genetic causes of male factor infertility: A review

Preimplantation genetic screening: does it help or hinder IVF treatment and what is the role of the embryo?

Lower sperm DNA fragmentation after r-FSH administration in functional hypogonadotropic hypogonadism

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