Sperm quality in naturally infected rams with Brucella ovis

Carrera‐Chávez, José Maria; Quezada‐Casasola, Andrés; Pérez-Eguía, Eduardo; Itzá-Ortíz, Mateo; Gutiérrez-Hernández, José Luis; Quintero-Elisea, Juan A.; Tórtora-Pérez, Jorge

doi:10.1016/j.smallrumres.2016.09.021

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…In the present study, the infection with Brucella spp. occurred naturally; considering this, the percentage of animals serologically positive that presented testicular lesion was lower (4.76%) than (30.56%) decelerated by Carrera-Chávez et al (2016).…”

Section: Discussionmentioning

confidence: 88%

“…Infected rams may have intermittent excretion or no excretion of bacteria, which makes bacteriological analysis not practical or reliable. The clinical diagnosis by scrotal palpation is valid only if the animal has developed lesions and should be considered that epididymitis may be caused by other bacteria; moreover, infected rams without apparent clinical signs, may excrete the bacteria (Ficapal et al, 1998;Mejid et al, 2010, Carrera-Chávez et al, 2016. Rams/bucks and teaser animals should comply with the requirements before entring into an artificial insemination center and isolation at the pre-entry isolation facility where the country or zone of origin is not free from the diseases.…”

Section: Introductionmentioning

confidence: 99%

“…Although authors have evaluated the relationship between palpable lesions of epididymitis and semen quality in rams (Carvalho et al, 2012), according to the literature review, sperm quality in rams serologically positive to Brucella spp. naturally infected has been evaluated before by Carrera-Chávez et al (2016). Therefore, this study aimed to evaluate sperm quality in naturally brucella-infected rams and goats which are also serologically positive for brucellosis and to isolate and identify Brucella spp.…”

Section: Introductionmentioning

confidence: 99%

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A Systematic Detection for Brucellosis at Chronic Stage of Infection in Semen of Sheep and Saanen Goats

Büyükcangaz

Üstüner

Erdenliğ

et al. 2019

Turkish JAF Sci.Tech.

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The study was conducted in a herd (n: 244) in which goats (n: 206) and sheep (n:38) had a history of brucellosis in Bursa which is located in Northwestern of Turkey between the years 2012-2014. For the detection of Brucella spp. and the other zoonotic bacterial agents, semen samples were taken from Saanen goats (n: 35) and rams (n: 8). Samples were tested by routine diagnostic procedures and PCR. The serum samples of male animals were also tested for Brucellosis by C-ELISA and I-ELISA. The culture results represented Trueperella pyogenes (n:2), Pasteurella pneumotopica (n: 5), Esherichia coli (n: 3), Aeromonas salmonicida subs. Salmonicida (1), Brevundimonas vesicularis (n: 2) and Mycoplasma bovigenitalium (n: 1) and Mycoplasma arginini (n: 1) from semen samples. Rams had no symptoms due to epididymitis or epididymoorchitis in clinical examination, but two bucks showed orchitis and they were serologically positive for brucellosis. Also, one seronegative buck showed epididymitis in a flock. There were no statistically significant differences between the serologically positive and negative animals in an examination of semen samples in terms of their volume, concentration, mass activity, motility and defectivity rate for acrosome. Although 20 of the serum samples were negative for anti-Brucella antibody, 23 of them were serologically positive for brucellosis. As a result of this study, Brucellae were not detected by bacteriologically and molecularly while there were some positive serum samples for brucellosis. This could be attributed that these samples might have been collected from chronically infected animals in which animals generally do not shed the organisms. Therefore, it was thought that sampling with regular intervals might help for the definitive incidence of brucellosis.

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Systematic Detection for Brucellosis at Chronic Stage of Infection in Semen of Sheep and Saanen Goats

Büyükcangaz

Üstüner

Erdenliğ

et al. 2019

Turkish JAF Sci.Tech.

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“…Unlike the majority of Brucellae, B.ovis does not have urease activity and cannot convert nitrate to nitrite. It is characterized by testicular changes, reduced fertility due to poor semen quality in rams (male sheep) and sporadic miscarriages in ewes (female sheep) (Blasco, 1990;Carrera-Chávez et al, 2016). The chronic phase of the disease in rams is characterized by testicular atrophy and varying degrees of epididymis tail expansion.…”

Section: Introductionmentioning

confidence: 99%

Unravelling key genes associated with ovine Brucellosis by differential gene expression analysis: A holistic bioinformatics study

Ramesh,

Indrabalan,

Rani

et al. 2024

VIS

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Ovine Brucellosis, caused by Brucella ovis bacteria, is a pathognomonic reproductive infectious disease of sheep that causes epididymitis in rams (male sheep) and placental inflammation in ewes (female sheep) leading to reduced fertility. The specific molecular process that causes alterations in genome of sheep during brucellosis is not yet fully understood. This study aimed to identify key host genes associated with the pathogenesis of ovine brucellosis caused by B. ovis. The GSE35614 dataset containing six healthy and six Brucella ovis infected sample of rams in the chronic phase 2 was obtained from the NCBI GEO database to examine and detect any differences in gene expression (DEGs). Functional and pathway enrichment analyse of the DEGs were performed along with the construction of protein-protein interaction network. Next, functional modules and hub genes were clustered and identified respectively, using the MCODE plugin. As a result, a total of 316 differentially expresses genes were filtered according to the provided cut-off criteria. The enriched DEGs were related to extracellular matrix interaction, cell adhesion mediated by integrin, angiogenesis, and inflammatory response. Furthermore, the hub gene analysis resulted in five hub genes namely, FN1, FBN1, CDH1, CD44, and SPP1, were up-regulated during the infection which could lead to reproductive disorders in sheep. In conclusion, the DEGs, functional and pathways terms, along with hub genes identified in the current study can provide prospective targets for the early diagnosis and treatment of brucellosis and provide insight into the molecular mechanism underlying the alterations that occur during brucellosis in sheep.

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Theriogenology of sheep, goats, and cervids

Edmondson¹,

Shipley²

2021

Sheep, Goat, and Cervid Medicine

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Sperm quality in naturally infected rams with Brucella ovis

Cited by 4 publications

References 13 publications

A Systematic Detection for Brucellosis at Chronic Stage of Infection in Semen of Sheep and Saanen Goats

A Systematic Detection for Brucellosis at Chronic Stage of Infection in Semen of Sheep and Saanen Goats

Unravelling key genes associated with ovine Brucellosis by differential gene expression analysis: A holistic bioinformatics study

Theriogenology of sheep, goats, and cervids

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