Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial

Malvezzi, Helena; Sharma, Rakesh; Agarwal, Ashok; Abuzenadah, Adel M.; Abu‐Elmagd, Muhammad

doi:10.1186/1477-7827-12-121

Cited by 59 publications

(39 citation statements)

References 35 publications

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“…PureCeption™ and Isolate™ are other commercially available density gradient media, both consisting of a colloidal suspension of silica particles stabilized with covalently bound hydrophilic silane in HEPES-buffered human tubal fluid (HTF). No significant increases in sperm DNA damage were observed in the lower layer after DGC using these media [Malvezzi et al 2014]. Therefore, a similar outcome was expected using these media in our study.…”

Section: Discussionsupporting

confidence: 88%

“…Generally, a motile mature spermatozoon has a higher density (1.10 g/mL) than an immotile (1.06 g/mL) or immature spermatozoon (1.09 g/mL) [Oshio et al 1987]. After centrifugation, leukocytes and cell debris are concentrated in the interface between the seminal plasma and upper layer, and morphologically abnormal spermatozoa collect in the interface between the upper and lower layer, while motile and mature spermatozoa form a pellet at the bottom of the tube [Malvezzi et al 2014]. However, the antioxidants contained in seminal plasma are also removed, increasing net ROS production by spermatozoa and leukocytes [Iwasaki and Gagnon 1992].…”

Section: Introductionmentioning

confidence: 99%

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Effect of density gradient centrifugation on reactive oxygen species in human semen

Takeshima

Yumura

Kuroda

et al. 2017

Systems Biology in Reproductive Medicine

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ROS: reactive oxygen species; SOD: superoxide dismutase; GPx: glutathione peroxidase; DNA: deoxyribonucleic acid; DGC: density gradient centrifugation; IUI: intrauterine insemination; IVF: in vitro fertilization; HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; EDTA: ethylenediaminetetraacetic acid; HTF: HEPES-buffered human tubal fluid; IMSI: intracytoplasmic morphologically selected sperm injection; SMAS: sperm motility analyzing system; CASA: computer-assisted semen analyzer; WHO: World Health Organization.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Effect of density gradient centrifugation on reactive oxygen species in human semen

Takeshima

Yumura

Kuroda

et al. 2017

Systems Biology in Reproductive Medicine

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“…In general, DGC technique showed results higher than other techniques as a result of saline coated silica that used for sperm preparation, and these result are in agreements with the findings of other studies that reported by the main improving role of this technique on the recovery of motile spermatozoa (15). In this study, the best choice to prepare or select functional sperm depending on the feature of the semen samples for all the three groups of samples Asthenozoospermic, Oligozoospermic and Normozoospermic samples is DGC technique specially when combined with TAD 600mg Glutathione, in which yielded high result for sperm motility and morphology more than other techniques.…”

Section: Discussionsupporting

confidence: 91%

Untitled

2018

IJEIR

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Background: One of the major concerns among the male reproductive health is the inability to achieve parenthood even after regular unprotected intercourse. The efficiency of the sperm selection techniques is expressed as the concentration of spermatozoa with normal motility. Discontinuous density gradient centrifugation (DGC) method widely used over the last decade due to effectiveness and reproducibility in recovering most motile sperm, which separates sperm based on their motility, size, and density differential. Glutathione act as an amino acid donor during spermatogenesis. If the levels of glutathione are too low during spermatogenesis, the number of mature and morphologically normal spermatozoa generated will be decreased. Objectives:The aim of this study was to assess the beneficial role of TAD 600mg Glutathione combined with DGC technique for activation of human sperm of asthenozoospermic, oligozoospermic and normozoospermic subjects compared with the DGC technique alone. Patients, Materials and Methods:Sixty males were involved in this study, divided into three groups, (twenty asthenozoospermic, Twenty oligozoospermic and twenty normozoospermic subjects)during their attendance to the Infertility Clinic at High Institute for Infertility Diagnosis and Assisted Reproductive Technologies; Al-Nahrain University. Semen samples were obtained, and seminal fluid analysis was assessed. Each semen sample was divided into three parts. The first part prepared as in vitro sperm characterization before activation, the second part using density gradient centrifugation technique, while the last part prepared using density gradient centrifugation combined with TAD 600mg Glutathione. Results: After in vitro sperm activation for asthenozoospermic, oligozoospermic and normozoospermic samples a significant increase was observed in the sperm function parameters including sperm concentration, motility and morphologically normal sperm percentage as compared to pre-activation using combined techniques (density gradient centrifugation and TAD 600mg Glutathione) as compared to DGC technique alone. Conclusions:TAD 600mg Glutathione combined to the DGC technique was found to give higher significant results on sperm function parameters (sperm concentration, motility and morphology) when using a low quality of semen samples such as decreased sperm motility as compared with DGC technique alone.

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“…In general , density gradient centrifugation technique showed result high than other techniques as a result of silane coated silica that used for sperm preparation, and these result agreements with the findings of other studies that reported by the main improving role of this technique on the recovery of motile spermatozoa (21,22,23,24) .…”

supporting

confidence: 82%

The New Beneficial Application of Caffeine as a Promoting Factor of Sperm Motility Combined With Density Gradient Centrifugation Technique for Best Yield for Asthenozoospermic and Oligozoospermic Men.

Hamza¹,

Selman²,

Mossa.³

2017

IJAR

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Background: Seminal fluid of males is varied in quality, not only between males but also within a single sample. A verity of in vitro sperm preparation techniques were developed to separate normal and motile spermatozoa from other constituents of sample to provide successful assisted reproductive techniques. Objectives:The goal of this study was to compare the effects of the four in vitro sperm activation techniques on sperm function parameters specially sperm motility ofasthenozoospermicand oligozoospermicsemen samples. Subjects, Materials and Methods:Thirty-five asthenozoospermic and fifteen oligozoospermic men were participated in this study during their attendance to the Infertility Clinic at High Institute for Infertility Diagnosis and Assisted Reproductive Technologies; Al-Nahrain University. Collection of semen samples and seminal fluid analysis was done according to WHO (1999) and WHO (2010). Each semen sample was divided into four parts. The first one prepared as in vitro sperm activation using the direct swim-up technique, the second using indirect swim-up technique,the third using density gradient centrifugation technique, while the last part prepared using density gradient centrifugation and Caffeine(combined techniques). Results: A significant increase was observed in the sperm function parameters including sperm motility and morphologically normal sperm for asthenozoospermic and oligozoospermic samples when using density gradient centrifugation and Caffeine as compared to density gradient centrifugation alone.Also, there was a significant increase was observed in the same sperm function parameters when using density gradient centrifugation as compared to direct swim-up technique and indirect swim-up technique. Conclusion: In this study, the outcome of sperm function parameters using combined density gradient technique with Caffeine were higher than other techniques, especially when using a low quality of semen samples such as decreased sperm motility, therefore it was fit in cases ofasthenozoospermia and oligozoospermia which are taken as examples of infertile group in this study.

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Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial

Cited by 59 publications

References 35 publications

Effect of density gradient centrifugation on reactive oxygen species in human semen

Effect of density gradient centrifugation on reactive oxygen species in human semen

Untitled

The New Beneficial Application of Caffeine as a Promoting Factor of Sperm Motility Combined With Density Gradient Centrifugation Technique for Best Yield for Asthenozoospermic and Oligozoospermic Men.

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