Sperm plasma membrane ion transporters and male fertility potential: A perspective under the prism of cryopreservation

Antonouli, Sevastiani; Di Nisio, Valentina; Messini, Christina; Samara, Maria; Salumets, Andres; Daponte, Alexandros; Anifandis, George

doi:10.1016/j.cryobiol.2023.104845

Cited by 3 publications

(1 citation statement)

References 90 publications

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“…Within the female reproductive tract, the intracellular Ca 2+ flux in sperm is tightly regulated by CatSper. An increase in intracellular Ca 2+ flux, mediated by CatSper, can induce sperm hyperactivation, acrosome reaction, oocyte chemotaxis, and zona pellucida penetration during the fertilization process [ 191 ]. However, the expression of CatSper is reduced during sperm cryopreservation [ 192 , 193 ], suggesting that the elevation of Ca 2+ flux in cryopreserved sperm is independent of CatSper.…”

Section: Regulated Cell Death and Sperm Freezing Damagementioning

confidence: 99%

Sperm freezing damage: the role of regulated cell death

Hai,

Li,

Zhang

et al. 2024

Cell Death Discov.

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Substantial progress in research on sperm cryopreservation has occurred since the twentieth century, especially focusing on improving sperm freezing procedures and optimizing semen extenders. However, the cellular biological mechanisms of sperm freezing damage are still unclear, which greatly restricts the promotion and development of sperm cryopreservation. An essential component of sperm freezing damage is the occurrence of cell death. Considering the existence of multiple types of cell death pathways, this review discusses connections between characteristics of regulated cell death (e.g., apoptosis and ferroptosis), and accidental cell death (e.g., intracellular ice crystals) with sperm freezing damage and explores possible future research directions in this field.

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Section: Regulated Cell Death and Sperm Freezing Damagementioning

confidence: 99%

Sperm freezing damage: the role of regulated cell death

Hai,

Li,

Zhang

et al. 2024

Cell Death Discov.

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Bidirectional Mendelian randomization to explore the causal relationships between the gut microbiota and male reproductive diseases

Han,

Tian,

Yang

et al. 2024

Sci Rep

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Gut bacteria might play an important role in male reproductive disorders, such as male infertility and sperm abnormalities; however, their causal role is unclear. Herein, Mendelian randomization (MR)-Egger, weighted median, inverse variance weighting, Simple mode, and Weighted mode were used to test the causal relationship between gut microbes and male reproductive diseases. The MR results were validated using various metrics. The MR results were also consolidated using reverse causality speculation, conducted using two-way MR analysis and Steiger filtering. Biological function was analysed using enrichment analyses. The results suggested that eight intestinal microflorae were causally associated with male infertility. The Eubacterium oxidoreducens group was associated with an increased risk of male infertility, while the family Bacteroidaceae was negatively associated with male reproductive diseases. Eight intestinal microflorae were causally associated with abnormal spermatozoa. The family Streptococcaceae was associated with a high risk of abnormal spermatozoa, whereas the family Porphyromonadaceae was associated with a low risk of abnormal spermatozoa. No pleiotropy was observed, this study identified a high correlation between the gut flora and the likelihood of male reproductive diseases. Future research will attempt to advance microbial-focused treatments for such diseases.

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Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures

Xu,

Sun,

et al. 2024

Animals

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Y-27632, as a cytoskeleton protector, is commonly used for low-temperature preservation of cells. Goat sperm are prone to damage to the cytoskeleton under low-temperature conditions, leading to a loss of sperm vitality. However, the Y-27632 small molecule has not yet been used in research on low-temperature preservation of goat semen. This study aims to address the issue of low temperature-induced loss of sperm motility in goats by using Y-27632, and explore the regulation of Y-27632 on goat sperm metabolism. At a low temperature of 4 °C, different concentrations of Y-27632 were added to the sperm diluent. The regulation of Y-27632 on the quality of low temperature-preserved goat semen was evaluated by detecting goat sperm motility, antioxidant capacity, mitochondrial activity, cholesterol levels, and metabolomics analysis. The results indicated that 20 µM Y-27632 significantly increased plasma membrane integrity (p< 0.05), and acrosome integrity (p < 0.05) and sperm motility (p < 0.05), increased levels of superoxide dismutase (SOD) and catalase (CAT) (p < 0.01), increased total antioxidant capacity (T-AOC) (p < 0.05), decreased levels of malondialdehyde (MDA) and reactive oxygen species (ROS) (p < 0.01), and significantly increased mitochondrial membrane potential (MMP). The levels of ATP, Ca2+, and TC in sperm increased (p < 0.01). Twenty metabolites with significant differences were identified, with six metabolic pathways having a significant impact, among which the D-glutamic acid and D-glutamine metabolic pathways had the most significant impact. The artificial insemination effect of goat semen treated with 20 μM Y-27632 was not significantly different from that of fresh semen. This study indicates that Y-27632 improves the quality of low-temperature preservation of sperm by protecting the sperm plasma membrane, enhancing sperm antioxidant capacity, regulating D-glutamine and D-glutamate metabolism, and promoting the application of low-temperature preservation of semen in artificial insemination technology.

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Sperm plasma membrane ion transporters and male fertility potential: A perspective under the prism of cryopreservation

Cited by 3 publications

References 90 publications

Sperm freezing damage: the role of regulated cell death

Sperm freezing damage: the role of regulated cell death

Bidirectional Mendelian randomization to explore the causal relationships between the gut microbiota and male reproductive diseases

Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures

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