Sperm-perivitelline membrane egg binding assay after addition of Ringer Lactate, Sodium Citrate 2.92 % and TRIS Solution in thawed goat semen

Abstract

RESUMO - (CBRA,1998). A capacidade de ligação dos espermatozoides à membrana perivitelina da gema do ovo foi semelhante entre os tratamentos (P>0,05), mas foi observada correlação positiva entre a motilidade espermática e a capacidade de ligação (P<0,05). A diluição do sêmen criopreservado com soluções isoosmóticas após o descongelamento pode ser útil na manutenção da qualidade espermática e no aumento do volume existente a fim de otimizar a realização de testes e biotecnologias reprodutivas in vitro. Palavras-chave: bode, diluente, espermatozoide.ABSTRACT -The present study was verified by testing for binding of sperm to the perivitelline membrane of the egg yolk fertilizing capacity of these cells after thawing and addition of Ringer Lactate, Sodium Citrate 2.92% and TRIS Solution. The semen of animals was collected and performed the standard procedures of analysis and cryopreservation seminal. After thawing was added the solutions Ringer Lactate, Sodium Citrate 2.92% and TRIS, which contained protective substances to sperm and performed the tests in vitro SpermPerivitelline Membrane Egg Binding and Morphology. The seminal parameters of fresh semen were recommended according to current legislation. The binding capacity of the sperm membrane perivitelline egg yolk was similar between treatments (P>0,05), but there was a positive correlation between sperm motility and binding capacity (P<0,05). Thus, the dilution of semen cryopreserved with solutions isoosmóticas after thawing can be useful in maintaining the quality of sperm and increase existing volume to optimize the testing and reproductive biotechnologies in vitro.