Sperm motility patterns of Atlantic cod (<i>Gadus morhua</i>) in relation to salinity: effects of ovarian fluid and egg presence

Litvak, Matthew K.; Trippel, Edward A.

doi:10.1139/f98-093

Cited by 79 publications

(79 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Salmonids are known to have one of the briefest fertile windows among fish and the period of sperm survival is short when observed in water (Vladic and Järvi, 1997, own observations). Yet, the ovarian fluid represents a protective environment (Litvak and Trippel, 1998) and Arctic charr ejaculates have a higher percentage of motile sperm cells in ovarian fluid than in water (Turner and Montgomerie, 2002). Moreover, the ovarian fluid seems to represent a selective environment for sperm (Yeates et al, 2013), and there is a strong interaction effect of ovarian fluid on sperm swimming speed in charr with certain female fluids stimulating swimming speed of sperm from some males over others (Urbach et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

On the relative effect of spawning asynchrony, sperm quantity, and sperm quality on paternity under sperm competition in an external fertilizer

et al. 2015

View full text Add to dashboard Cite

Citation:Egeland TB, Rudolfsen G, Nordeide JT and Folstad I (2015) On the relative effect of spawning asynchrony, sperm quantity, and sperm quality on paternity under sperm competition in an external fertilizer. Front. Ecol. Evol. 3:77. doi: 10.3389/fevo.2015.00077 On the relative effect of spawning asynchrony, sperm quantity, and sperm quality on paternity under sperm competition in an external fertilizer How much of a fitness benefit is obtained by dominant males of external fertilizers from releasing ejaculates in synchrony with female egg-release when engaging in sperm competition, and what is the most important sperm trait for paternity in these situations? The Arctic charr (Salvelinus alpinus) is an external fertilizer experiencing intense male-male competition over reproductive opportunities including sperm competition. To compensate for their disadvantage the sneaker males, which often spawn out of synchrony with the female, produce more and faster sperm than the guarding males. We used controlled in vitro fertilization trials with experimentally produced dominant and subordinate, sneaker males to test what effect relative synchrony in gamete release, sperm quality (i.e., motility and velocity) and sperm quantity have on a male's fertilization success in pair-wise sperm competitions. When the sneaker males released ejaculates after the guarding male there was no overall difference in fertilization success. The quality (i.e., motility and velocity) of a male's sperm relative to that of the competing male was the best predictor of male fertilization success regardless of their mating tactic and spawning synchrony. The relative number of sperm cells also had an effect on fertilization success, but mainly when the dominant and sneaker male ejaculated synchronously. Our close imitation of natural sperm competition in charr shows that the sneaker males of external fertilizing species may fully compensate for their disadvantaged mating role by producing ejaculates of higher quality-an adjustment strangely not met by dominants.

show abstract

Section: Discussionmentioning

confidence: 99%

On the relative effect of spawning asynchrony, sperm quantity, and sperm quality on paternity under sperm competition in an external fertilizer

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The role that ovarian fluid plays in influencing motility and fertilization success has recently been studied (Litvak and Trippel, 1998). It was revealed that cod sperm motility is enhanced when diluted semen was placed in ovarian fluid or directly on the egg's surface still coated by ovarian fluid compared to seawater alone.…”

Section: Application Of Sperm Motility Data To Estimation Of Stockviamentioning

confidence: 99%

“…Eggs, ovarian fluid, sperm, seminal fluid and seawater become mixed when genital pores are closely aligned during spawning. The ovarian fluid contribution to the gamete mixture may improve chances for fertilization, such that sperm can be activated or their velocity enhanced when in contact with maternal substances (Amanze and Iyengar, 1990;Litvak and Trippel, 1998). Note, turbot sperm motility (duration) approximately doubled when males were kept in captivity with other females as compared to being in isolation (Suquet et al, 1992b) and a lengthier period of herring sperm motility occurred in the presence of eggs (Geffen, 1999).…”

Section: Application Of Sperm Motility Data To Estimation Of Stockviamentioning

confidence: 99%

Estimation of Male Reproductive Success of Marine Fisheries

Trippel¹

2003

J. Northw. Atl. Fish. Sci.

View full text Add to dashboard Cite

The subject of reproductive potential of fish populations is dominated by studies on the female gender. Studies on male reproduction are relatively few, but have increased in number during the previous decade. The objectives of this contribution were to describe and quantify the reproductive traits that make up the viable sperm production of a population. Some of these reproductive traits were easily measured from wild fish (e.g., mature testes weight), and others were more easily measured on captive fish (e.g., fertilization potential and sperm motility). Results of laboratory and field studies were then integrated to generate estimates of viable sperm production of a fish stock. A number of experimental protocols have been employed over the years to assess male fertility. The strengths and weaknesses of the different experimental approaches were reviewed and appropriate recommendations given towards establishing standardized protocols. Although the review is broad in nature, and includes references to a number of marine fishes, it concentrated on exploited species that occur in the North Atlantic and Baltic Sea within the taxonomic groups gadoidae, pleuronectoidae and clupeidae (Clupeiformes). In addition, published data on male reproductive traits of species in these taxa were tabulated and summarized. The terms of interest included sex ratio, maturity state, testes weight, sperm fertilization potential (artificial fertilization and paired mating), sperm density, sperm motility and paternal effects on early life history traits.An equation for determinate spawners was described that quantifies a population's viable sperm production using data on adult body characteristics and associated quantity and quality of sperm. The equation was used to establish time series of viable sperm production for Atlantic cod (Gadus morhua) of Newfoundland and Labrador. Results indicated on average 1 600 billion viable sperm were released to produce one recruit at age 3 years but with substantial variation among years in relation to changes in stock composition and growth as well as stock size. Distinct peaks in the number of viable sperm in 1981 and 1986 corresponded to distinct peaks in the number of age 3 recruits in those same years.

show abstract

“…Prior work in several fish species has shown that ovarian fluid, a viscous substance surrounding the spawned ova, enhances sperm longevity, per cent motility, path linearity and sperm velocity compared to water (for example, Litvak and Trippel, 1998;Turner and Montgomerie, 2002;Rosengrave et al, 2008;Galvano et al, 2013). The degree of stimulation of sperm velocity by the ovarian fluid is greatly explained by male-female interactions, which is indicative of the presence of CFC acting to promote gamete fusions of particular female-male combinations (Urbach et al, 2005;Rosengrave et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Sexual selection for genetic compatibility: the role of the major histocompatibility complex on cryptic female choice in Chinook salmon (Oncorhynchus tshawytscha)

et al. 2017

View full text Add to dashboard Cite

Cryptic female choice (CFC), a form of sexual selection during or post mating, describes processes of differential sperm utilization by females to bias fertilization outcomes towards certain males. In Chinook salmon (Oncorhynchus tshawytscha) the ovarian fluid surrounding the ova of a given female differently enhances the sperm velocity of males. Sperm velocity is a key ejaculate trait that determines fertilization success in externally fertilizing fishes, thus the differential effect on sperm velocity might bias male fertilization outcomes and represent a mechanism of CFC. Once sperm reach the oocyte, CFC could potentially be further facilitated by sperm-egg interactions, which are well understood in externally fertilizing marine invertebrates. Here, we explored the potential genetic basis of both possible mechanisms of CFC by examining whether the genotypic combinations of mates (amino-acid divergence, number of shared alleles) at the major histocompatibility complex (MHC) class I and II explain the variation in sperm velocity and/or male fertilization success that is not explained by sperm velocity, which might indicate MHC-based sperm-egg interactions. We recorded sperm velocity in ovarian fluid, employed paired-male fertilization trials and evaluated the fertilization success of each male using microsatellite-based paternity assignment. We showed that relative sperm velocity was positively correlated with fertilization success, confirming that the differential effect on sperm velocity may be a mechanism of CFC in Chinook salmon. The variation in sperm velocity was independent of MHC class I and II. However, the MHC class II divergence of mates explained fertilization success, indicating that this locus might influence sperm-egg interactions.

show abstract

Sperm motility patterns of Atlantic cod (Gadus morhua) in relation to salinity: effects of ovarian fluid and egg presence

Cited by 79 publications

References 24 publications

On the relative effect of spawning asynchrony, sperm quantity, and sperm quality on paternity under sperm competition in an external fertilizer

On the relative effect of spawning asynchrony, sperm quantity, and sperm quality on paternity under sperm competition in an external fertilizer

Estimation of Male Reproductive Success of Marine Fisheries

Sexual selection for genetic compatibility: the role of the major histocompatibility complex on cryptic female choice in Chinook salmon (Oncorhynchus tshawytscha)

Contact Info

Product

Resources

About