Sperm motility patterns and metabolism in Catalonian donkey semen

Miró, Jordi; Lobo, Vivian; Moreno, Armando Quintero; Medrano, A.; Peña, Alejandro; Rigau, Teresa

doi:10.1016/j.theriogenology.2004.07.022

Cited by 77 publications

(37 citation statements)

References 21 publications

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“…Examination of stoichiometric ratios revealed that 60%-70% of produced lactate could have been explained on the basis of pyruvate uptake and reduction (in contrast to only 0%-40% generated from exogenous glucose catabolism). These are important observations given that lactate production (measured by enzyme-linked fluorescence) has been used as an indicator of glycolytic metabolism for other species [25,30,31]. Ongoing research in our laboratory supports this mechanism of lactate production in felid spermatozoa.…”

Section: Discussionsupporting

confidence: 58%

“…Therefore, glycolysis may be an important supplemental source of ATP to fuel sperm motility, and glycolytic enzymes have been localized along the fibrous sheath of the flagellum in the boar, bull, rat, stallion, human, and mouse [28,29]. Sperm production of lactate (presumably by glycolysis) is correlated positively with motility, normal morphology, acrosomal integrity, and osmotic resistance in the boar and donkey [30,31]. One of the latter studies has suggested that these relationships are more than casual in that litter size in pigs is enhanced after artificial insemination using sperm producing high lactate concentrations [30].…”

Section: Introductionmentioning

confidence: 99%

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Evidence for Compromised Metabolic Function and Limited Glucose Uptake in Spermatozoa from the Teratospermic Domestic Cat (Felis catus) and Cheetah (Acinonyx jubatus)1

Terrell

Wildt

Anthony

et al. 2010

Biology of Reproduction

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Cheetahs and certain other felids consistently ejaculate high proportions (!60%) of malformed spermatozoa, a condition known as teratospermia, which is prevalent in humans. Even seemingly normal spermatozoa from domestic cat teratospermic ejaculates have reduced fertilizing capacity. To understand the role of sperm metabolism in this phenomenon, we conducted a comparative study in the normospermic domestic cat versus the teratospermic cat and cheetah with the general hypothesis that sperm metabolic function is impaired in males producing predominantly pleiomorphic spermatozoa. Washed ejaculates were incubated in chemically defined medium containing glucose and pyruvate. Uptake of glucose and pyruvate and production of lactate were assessed using enzyme-linked fluorescence assays. Spermatozoa from domestic cats and cheetahs exhibited similar metabolic profiles, with minimal glucose metabolism and approximately equimolar rates of pyruvate uptake and lactate production. Compared to normospermic counterparts, pyruvate and lactate metabolism were reduced in teratospermic cat and cheetah ejaculates, even when controlling for sperm motility. Rates of pyruvate and lactate (but not glucose) metabolism were correlated positively with sperm motility, acrosomal integrity, and normal morphology. Collectively, our findings reveal that pyruvate uptake and lactate production are reliable, quantitative indicators of sperm quality in these two felid species and that metabolic function is impaired in teratospermic ejaculates. Furthermore, patterns of substrate utilization are conserved between these species, including the unexpected lack of exogenous glucose metabolism. Because glycolysis is required to support sperm motility and capacitation in certain other mammals (including dogs), the activity of this pathway in felid spermatozoa is a target for future investigation.

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Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Evidence for Compromised Metabolic Function and Limited Glucose Uptake in Spermatozoa from the Teratospermic Domestic Cat (Felis catus) and Cheetah (Acinonyx jubatus)1

Terrell

Wildt

Anthony

et al. 2010

Biology of Reproduction

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“…All the sperm parameters assessed in fresh semen samples used in this study were within the physiological values for donkey semen evaluation [17]: sperm concentration greater than 208.7 Â 10 6 sperm/mL, gel-free volume greater than 44 mL, sperm TM greater than 90.3%, and sperm PM greater than 67.3%.…”

Section: Resultsmentioning

confidence: 65%

Effect of single-layer centrifugation or washing on frozen–thawed donkey semen quality: Do they have the same effect regardless of the quality of the sample?

et al. 2015

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“…Analyses were carried out with logtransformed variables. Data for straightline velocity (VSL) were obtained from the literature (Mortimer and Mortimer, 1990;Stachecki et al, 1993Stachecki et al, , 1994Shivaji et al, 1995;Mahonynet al, 1996;Moore and Akhondi, 1996;Trimeche et al, 1996Trimeche et al, , 1999Patil et al, 1998;van der Horst et al, 1999;Castellini et al, 2000Castellini et al, , 2006Jayaprakash et al, 2001;Kawaguchi et al, 2004;Lavara et al, 2005;Miró et al, 2005;Baumber and Meyers, 2006;Cox et al, 2006;Sloter et al, 2006;Aurich et al, 2007;Grzmil et al, 2007;Holt et al, 2007;Hung et al, 2007;Immler et al, 2007a;Rijsselaere et al, 2007) and our own unpublished observations. Data for total sperm length were obtained from Cummins and Woodall (1985), Cassinello et al (1998), Gage and Freckelton (2003) and Malo et al (2006 Pitnick et al, 2001).…”

Section: ) (C)mentioning

confidence: 99%

Implications of diversity in sperm size and function for sperm competition and fertility

Gomendio

Roldán²

2008

Int. J. Dev. Biol.

167

185

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Sperm competition is now recognised as a potent selective force shaping many male reproductive traits. While the influence of sperm competition on sperm number is widely accepted, its effects upon sperm size remain controversial. It had been traditionally assumed that there is a trade-off between sperm number and sperm size, so that an increase in sperm number would result in a decrease in sperm size, under conditions of sperm competition. Contrary to this prediction, we proposed some time ago that sperm competition favours an increase in sperm size, because longer sperm swim faster and are more likely to win the race to fertilize ova. Comparative studies between species show that in many taxa such a relationship exists, but the consequences of an increase in sperm size may vary between taxa depending on the environment in which spermatozoa have to compete. We present new evidence showing that in mammals longer sperm swim at higher speeds. We also show that mean swimming speed is highly correlated with maximum swimming speed, so even if the fastest swimming sperm are more likely to fertilize, both measures are informative. When individuals of the same species are compared, ratios between the dimensions of different sperm components, as well as the shape of the head, seem better at explaining sperm swimming velocity. Finally, we show that mean and maximum sperm swimming speed determine male fertility. Other studies have shown that in competitive contexts, males with faster swimming sperm have higher fertilization success. We conclude that the available evidence supports our original hypothesis.

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Sperm motility patterns and metabolism in Catalonian donkey semen

Cited by 77 publications

References 21 publications

Evidence for Compromised Metabolic Function and Limited Glucose Uptake in Spermatozoa from the Teratospermic Domestic Cat (Felis catus) and Cheetah (Acinonyx jubatus)1

Evidence for Compromised Metabolic Function and Limited Glucose Uptake in Spermatozoa from the Teratospermic Domestic Cat (Felis catus) and Cheetah (Acinonyx jubatus)1

Effect of single-layer centrifugation or washing on frozen–thawed donkey semen quality: Do they have the same effect regardless of the quality of the sample?

Implications of diversity in sperm size and function for sperm competition and fertility

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