Sperm Motility in Mytilus Edulis in Relation to Mitochondrial Dna Polymorphisms: Implications for the Evolution of Doubly Uniparental Inheritance in Bivalves

Jha, Mamta; Côté, Jean-Charles; Hoeh, Walter R.; Blier, Pierre; Stewart, Donald T.

doi:10.1111/j.1558-5646.2007.00262.x

Cited by 29 publications

(59 citation statements)

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“…An initial test of this hypothesis by Everett et al (2004) indicated no significant differences in swimming speeds between sperm containing either the SM or RM mitotypes in M. edulis. However, a more recent analysis that used larger samples and computerassisted sperm analysis demonstrated that RM containing sperm were significantly faster than SM containing sperm (Jha et al, 2007). These results suggest that the RM mtDNA/nuDNA combination (which is essentially an F mtDNA/ nuDNA combination) results in faster spermatozoa than the SM mtDNA/nuDNA combination.…”

mentioning

confidence: 74%

“…(5 0 -GGTCAACAAATCATAAAGA-TATTGG-3 0 ) and a modified version of HCO2198 that was five bases shorter in the 5 0 end (5 0 -TCAGGGTGACCAAAAAATCA-3 0 ) (Folmer et al, '94;Jha et al, 2007). These sequences (Jha et al, 2007; GenBank Accession Numbers EU018148 to EU018204) were compared with previously characterized RM and SM mitotypes as well as a standard female (F) mitotype that were downloaded from the GenBank database (Accession Numbers AY713960, AY130053 and U68773).…”

Section: Mitotyping and Genotypingmentioning

confidence: 99%

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Role‐reversal of gender‐associated mitochondrial DNA affects mitochondrial function in Mytilus edulis (Bivalvia: Mytilidae)

2008

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Mussel species of the genus Mytilus possess an unusual system of mitochondrial DNA (mtDNA) transmission termed doubly uniparental inheritance. They are characterized by the presence of two highly divergent gender-associated mtDNA genomes (often with>20 and>10% divergences in DNA and amino acid sequences, respectively) that are inherited either maternally (F mtDNA) or paternally (M mtDNA). Females are typically homoplasmic for the F mtDNA and males are heteroplasmic with the F mtDNA being most common in all tissues except the gonad that is dominated by the M mtDNA. Collectively, males are polymorphic for two classes of M mtDNAs known as the "standard male" and "recently masculinized" M types (SM and RM, respectively). The coding portions of the RM mtDNA genome differ from the SM mtDNA by as much as the maternally inherited F mtDNA genome differs from the SM type. Because the SM and RM types exhibit considerable amino acid sequence divergence, we hypothesized that these differences could affect mitochondrial respiratory chain complex enzyme activities. To test this hypothesis, the activity of the major mitochondrial respiratory chain complexes (complexes II, I+III and IV) as well as the activity of citrate synthase were measured on gonad samples from males containing either the SM or RM mtDNA. Our data demonstrate that the mitochondrial subunits encoded by the RM mtDNA are associated with higher enzymatic activities than the gene products of the SM mtDNA.

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confidence: 74%

Section: Mitotyping and Genotypingmentioning

confidence: 99%

Role‐reversal of gender‐associated mitochondrial DNA affects mitochondrial function in Mytilus edulis (Bivalvia: Mytilidae)

2008

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“…The mean sperm movement characteristics measured by Faure (1996) on scallop sperm are close to those of the present work, except for linearity which was higher (0.65 to 0.75), suggesting a less circular path assessed in this previous study which used a different CASA device (Modcell) than that one used in the present work (Image J). Compared to scallop, lower values of VAP were reported in blue mussels (Mytilus edulis): 103 ± 2 µm s −1 (Jha et al 2007) or Pacific oyster: 72 ± 14 µm s −1 (Faure 1996). On the other hand, a higher swimming speed was assessed in black-lip-pearl oysters: 221 ± 12 µm s −1 (Demoy-Schneider et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…The characteristics of sperm, collected after mollusk sperm shedding, were estimated in a few species: mussel (Mytilus edulis, Everett et al 2004;Jha et al 2007), Pacific oyster (Cosson et al 2008a;Suquet et al 2012) and sea urchin (Anthocidaris crassispina; Au et al 2000). Sperm fertilisation capacity of two Caribbean scallops (Argopecten nucleus and Nodipecten nodosus) was not influenced by stimulation technique, including temperature shock and serotonin or prostaglandin injection (Velasco et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm

Suquet

Quéré

Mingant

et al. 2013

Aquat. Living Resour.

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-Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (VAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, VAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed after serotonin injection. From the distal part of testes up to the gonopore, an increase of the percentage of motile spermatozoa and VAP was assessed, suggesting a sperm 'maturation process' along the genital ducts. A higher increase in the percentage of motile sperm was recorded during a 5 min incubation of testicular sperm in seawater containing 2 mM serotonin and seawater containing 10 mM caffein compared to seawater (control). In addition, a higher VAP was assessed, incubating testicular sperm in caffein, compared to control or serotonin. Then, the percentage of motile spermatozoa, VAP and intracellular ATP content exhibited a progressive reduction during the 10 h swimming period. Mean values of the percentage of motile spermatozoa, VAP, sperm track linearity (LIN) and intracellular ATP content recorded at the beginning of the movement period for sperm samples collected after intragonadal serotonin injection, were 82 ± 7%, 162 ± 15 µm s −1 , 0.33 ± 0.12 and 212 ± 133 nmol ×10 −9 spermatozoa (n = 9 males), respectively. The present study confirms the existence of a sperm "maturation process" along scallop genital ducts. In addition, the cessation of scallop sperm movement can be explained by the exhaustion of ATP content at the end of the movement phase.

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“…Countering this inference is the demonstration that in Medicago (clover) (Matsushima et al 2008), Passiflora (Hansen et al 2007), and Mytilus (mussel) (Jha et al 2008), organelle inheritance is or can be biparental without known adverse consequences. Clearly there are fundamental features of UP that are not yet understood, leading us to quote a quip from Maynard Smith (1976) regarding another sex-related enigma: "One is left with the feeling that some essential feature of the situation is being overlooked.…”

Section: Mating-type-regulated Transmission Of Organelle Genomes Theomentioning

confidence: 99%

Origins of Eukaryotic Sexual Reproduction

Goodenough

Heitman

2014

Cold Spring Harbor Perspectives in Biology

170

134

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Sexual reproduction is a nearly universal feature of eukaryotic organisms. Given its ubiquity and shared core features, sex is thought to have arisen once in the last common ancestor to all eukaryotes. Using the perspectives of molecular genetics and cell biology, we consider documented and hypothetical scenarios for the instantiation and evolution of meiosis, fertilization, sex determination, uniparental inheritance of organelle genomes, and speciation.

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Sperm Motility in Mytilus Edulis in Relation to Mitochondrial Dna Polymorphisms: Implications for the Evolution of Doubly Uniparental Inheritance in Bivalves

Cited by 29 publications

References 46 publications

Role‐reversal of gender‐associated mitochondrial DNA affects mitochondrial function in Mytilus edulis (Bivalvia: Mytilidae)

Role‐reversal of gender‐associated mitochondrial DNA affects mitochondrial function in Mytilus edulis (Bivalvia: Mytilidae)

Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm

Origins of Eukaryotic Sexual Reproduction

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