Sperm motility differences between donkey cooled sperm processed by colloid centrifugation

Ortiz, Isabel; Dorado, J.; Morrell, Jane M.; Acha, D.; Ramirez, L.; Urbano, M.; Carrasco, J.J.; Gómez-Arrones, V.; Calero, R.; Hidalgo, M.

doi:10.1016/j.jevs.2012.06.077

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“…Preliminary results of the use of SLC in donkey semen samples have been published by our research group (Ortiz et al, 2012); however, to our knowledge, this is the first full research article in which the effect of colloid centrifugation is tested in cooled-stored donkey sperm doses.…”

Section: Discussionmentioning

confidence: 99%

“…Sperm quality parameters, such as sperm motility, morphology and chromatin integrity are also improved in SLC-selected stallion semen samples using Androcoll-E-Large (Morrell et al, 2011a). However, to our knowledge, only preliminary results of the use of SLC to improve sperm motility in cooled donkey semen samples have been published by our research group (Ortiz et al, 2012). Despite common belief, the transfer of knowledge and procedures from horses to donkeys often achieves poor results (Contri et al, 2010b); so additional studies should be performed to evaluate if SLC previously used with great success in stallion semen (Morrell et al, 2011b) is suitable for donkey semen samples as well.…”

Section: Introductionmentioning

confidence: 98%

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Effect of single layer centrifugation using Androcoll-E-Large on the sperm quality parameters of cooled-stored donkey semen doses

Ortiz

Dorado

Ramirez

et al. 2014

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The aim of this study was to determine the effect of single layer centrifugation (SLC) using Androcoll-E-Large on donkey sperm quality parameters after 24 h of cool-storage. Ejaculates were collected from Andalusian donkeys and then cooled at 5°C. SLC was carried out after 24 h of cool-storage using Androcoll-E-Large. In the first experiment, all sperm parameters assessed (total and progressive sperm motility, viability, sperm morphology and sperm kinematics VCL, VSL, VAP, LIN, STR, WOB, ALH and BCF) were statistically compared between semen samples processed or not with Androcoll-E-Large. Significant differences ( P < 0.05) were found between SLC-selected and unselected semen samples for all parameters assessed, obtaining better results after SLC. In the second experiment, semen samples were classified in two groups according to their sperm progressive motility (PM) before SLC. Then, the increments obtained in semen quality parameters after SLC were compared between groups. No significant differences were found between groups, indicating that SLC improved the sperm quality parameters of entire set of semen samples processed with independence to their original PM. In conclusion, SLC with Androcoll-E-Large can be used in donkeys, increasing the sperm quality of cooled-stored donkey semen doses after 24 h of cool storage.

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Section: Discussionmentioning

confidence: 99%