Sperm motility changes after coincubation with various uropathogenic microorganisms: an in vitro experimental study

Berktaş, Mustafa; Aydın, Sabahattin; Yılmaz, Yüksel; Çeçen, Kürşat; Bozkurt, Hayrunnisa Bekis

doi:10.1007/s11255-007-9289-4

Cited by 38 publications

(27 citation statements)

References 18 publications

(25 reference statements)

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“…Sperm motility and vitality are decreased in patients with genital tract infection ( Diemer et al., ; Huwe et al ., ; Sanocka‐Maciejewska et al ., ; Berkast et al ., ). Escherichia coli has been isolated from prostatic secretion in 65–80% of chronic bacterial prostatitis cases (Weidner et al ., ) and from semen in 69% of cases of chronic bacterial prostatitis (Weidner et al ., 1991b).…”

Section: Introductionmentioning

confidence: 97%

Distinct isolates of uropathogenicEscherichia colidifferentially affect human sperm parametersin vitro

et al. 2013

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Sperm motility and vitality are decreased in male genital tract infection. Uropathogenic Escherichia coli (UPEC) are frequently associated with sperm parameter loss, but there are no reports to date regarding the effects of different E. coli isolates on human spermatozoa. The aim of this work was to compare the effect in vitro of different E. coli isolates on human sperm parameters. Normal spermatozoa were incubated with E. coli isolated from nine men with urinary tract infection. After 1 h of incubation, sperm motility, vitality and mitochondrial membrane potential (ΔΨm) were measured. The E. coli isolates were serotyped with specific antisera. Sperm motility was decreased with five of nine E. coli isolates. Two UPEC were typed as O6 strains, and they did not decrease sperm motility in the same experimental conditions as the other five isolates, despite the described high pathogenicity of the O6 strain in urogenital infections. Neither UPEC analysed affected vitality or ΔΨm. UPEC isolates were shown to be heterogeneous in their effects, suggesting the need to characterise the pattern defining the pathogenicity of E. coli on human spermatozoa.

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Section: Introductionmentioning

confidence: 97%

Distinct isolates of uropathogenicEscherichia colidifferentially affect human sperm parametersin vitro

et al. 2013

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“…A body of evidence records that a number of species of bacteria exert a negative impact on semen and sperm parameters including motility (Berktas et al ., ; Prabha et al ., ; De Francesco et al ., ), morphology (Eley et al ., ; Zhang et al ., ), concentration (Mazzoli et al ., ), DNA fragmentation (Gallegos et al ., ), acrosome reaction (Gupta & Prabha, ) and increased apoptosis (Eley et al ., ). The presence of bacteria was also recently found to be associated with infertility (Abusarah et al ., ).…”

Section: Introductionmentioning

confidence: 98%

Presence of aerobic micro-organisms and their influence on basic semen parameters in infertile men

et al. 2014

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Urogenital tract infections in males are one of the significant etiological factors in infertility. In this prospective study, 72 patients with abnormal semen parameters or any other symptoms of urogenital tract infection were examined. Semen analysis according to the WHO 2010 manual was performed together with microbial assessment: aerobic bacteria culture, Chlamydia antigen test, Candida culture, Ureaplasma and Mycoplasma-specific culture. In total, 69.4% of semen samples were positive for at least one micro-organism. Ureaplasma sp. was the most common micro-organism found in 33% of semen samples of infertile patients with suspected male genital tract infection. The 2nd most common micro-organisms were Enterococcus faecalis (12.5%) and Escherichia coli (12.5%), followed by Staphylococcus aureus (7%), Chlamydia trachomatis (7%) and Candida sp. (5.6%). Generally, bacteria were sensitive to at least one of the antibiotics tested. No statistically significant relationship was observed between the presence of aerobic micro-organisms in semen and basic semen parameters: volume, pH, concentration, total count, motility, vitality and morphology.

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“…The results of these studies suggest that the simple presence of bacteria in semen samples may compromise sperm quality (2, 3, 29). However, the majority of the data on the interactions between spermatozoa and bacteria are derived from in vitro studies (30), under conditions that may not accurately mimic in vivo conditions, For example, the bacterial population densities used for in vitro experiments have been much higher than ever recovered from ejaculate specimens (31). In some other studies, these putative pathogenic bacteria were not only found in the reproductive tracts of infertile patients, but also in those of healthy men (25, 26).…”

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confidence: 99%

Microbiota of the seminal fluid from healthy and infertile men

Hou

Zhou

Zhang

et al. 2013

Fertility and Sterility

217

227

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Objective: To explore potential causes of male infertility by determining the composition and structure of commensal bacterial communities in seminal fluids. Design: Microscopy of gram stained semen samples and classification of 16S rRNA gene sequences to determine the species composition of semen bacterial communities. Setting(s): Clinical andrology laboratory and academic research laboratories. Patient(s): 19 sperm donors and 58 infertility patients. Intervention(s): None. Main Outcome Measure(s): Classification of 16S rRNA gene sequences, clustering of seminal microbial communities, and multiple statistical tests. Result(s): High numbers of diverse kinds of bacteria were present in most samples of both sperm donors and infertility patients. The bacterial communities varied widely between subjects, but they could be clustered into six groups based on similarities in composition and the rank abundances of taxa. Overall there were no significant differences between sperm donors and infertility patients. However, multiple statistical tests showed a significant negative association between sperm quality and the presence of Anaerococcus. The results also indicated that many of the bacterial taxa identified in semen also occur in the vaginal communities of some women, especially those with bacterial vaginosis, which suggests heterosexual sex partners may share bacteria. Conclusion(s): Diverse kinds of bacteria were present in the human semen, there were no significant differences between sperm donors and infertility patients, The presence of Anaerococcus might be a biomarker for low sperm quality.

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Sperm motility changes after coincubation with various uropathogenic microorganisms: an in vitro experimental study

Cited by 38 publications

References 18 publications

Distinct isolates of uropathogenicEscherichia colidifferentially affect human sperm parametersin vitro

Distinct isolates of uropathogenicEscherichia colidifferentially affect human sperm parametersin vitro

Presence of aerobic micro-organisms and their influence on basic semen parameters in infertile men

Microbiota of the seminal fluid from healthy and infertile men

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