Sperm motility analysis system implemented on a hybrid architecture to produce an intelligent analyzer

Savkay, O. Levent; Yalçın, Müştak E.; Tavşanoglu, V.

doi:10.1016/j.imu.2020.100324

Cited by 1 publication

(4 citation statements)

References 27 publications

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“…Sperm crossing over each other can cause mismatch of the tracked spermatozoa. Commercial CASA systems are prone to tracking errors from sperm crossing over, 17,18 which results in disturbances to sperm's trajectories and hence motility measurement 19 . To achieve reliable tracking of individual spermatozoa even when they cross‐over each other, we have developed a shape‐based robust sperm tracker, which is built upon joint probabilistic data association filter (JPDAF) 20 .…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, many viruses, such as mumps virus, HIV and HSV, 7,14 have been found to impair semen quality, and they may directly interact with spermatozoa or affect spermatogenesis by inducing local inflammation. [15][16][17] Previous studies found that SARS, 1 of the 3 epidemic coronaviruses to emerge in the past 20 years and that shows similar clinical presentations to COVID-19, 18 could cause orchitis 19 and focal testicular atrophy. 20 Considering the tens of millions of COVID-19 cases and that men are more vulnerable to COVID-19 than women, [21][22][23] it is imperative to determine the effect of COVID-19 on male reproduction.…”

Section: Mean (±Standard Deviation)mentioning

confidence: 99%

“…Commercial CASA systems are prone to tracking errors from sperm crossing over, 17,18 which results in disturbances to sperm's trajectories and hence motility measurement. 19 To achieve reliable tracking of individual spermatozoa even when they cross-over each other, we have developed a shape-based robust sperm tracker, which is built upon joint probabilistic data association filter (JPDAF). 20 Since JPDAF only utilizes kinematics information (position and velocity) and is still prone to tracking errors when cross-over spermatozoa have similar positions and velocities, our sperm tracker incorporates additional shape information of spermatozoa (e.g.…”

Section: Automated Motility Measurementmentioning

confidence: 99%

“…1 of the 3 epidemic coronaviruses to emerge in the past 20 years and that shows similar clinical presentations to COVID-19, 18 could cause orchitis 19 and focal testicular atrophy. 20 Considering the tens of millions of COVID-19 cases and that men are more vulnerable to COVID-19 than women, [21][22][23] it is imperative to determine the effect of COVID-19 on male reproduction.…”

mentioning

confidence: 99%

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Automated motility and morphology measurement of live spermatozoa

et al. 2021

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Background: Automated sperm analysis has wide applications in infertility diagnosis.Existing systems are not able to measure sperm count and both motility and morphology of individual live spermatozoa. Morphology measurement requires invasive staining, making the spermatozoa after morphology measurement not applicable to infertility treatment.Objective: To evaluate the reproducibility and reliability of automated measurement of individual live sperm's motility and morphology.Materials and methods: Fresh semen samples were obtained from twenty male partners attending for fertility investigations. The system firstly measured motility for all the spermatozoa within the field of view under a low magnification (20×), then a spermatozoa of interest is selected by the user and automatically relocated by the system after switching to a high magnification (100×) for morphology measurement.Reproducibility of sperm measurements was evaluated by intraclass correlation coefficients on consecutive measurement. Reliability of motility and morphology measurement was evaluated by tracking error rate and limits of agreement, respectively, with manual measurement as benchmark.Results: Measurement of all motility and morphology parameters had intraclass correlation coefficients higher than 0.94. Sperm motility measurement had a tracking error rate of 2.1%. Limit of agreement analysis indicated that automated measurement and manual measurement of sperm morphology were interchangeable. Automated measurement of all morphology parameters was not statistically different from manual measurement, as confirmed by the paired sample t test.Discussion: Automated motility and morphology measurement of single sperm revealed high reproducibility and reliability. The system also achieved a high efficiency for motility and morphology measurement. In addition to the intracytoplasmic sperm injection (ICSI) samples with polyvinylpyrrolidone (PVP), the developed sperm measurement technique is also effective for analyzing semen and washed samples. The system provides a valuable tool for quantitative measurement and selection of single spermatozoa for ICSI. It can also be used for sperm motility and morphology analysis in andrology laboratories.

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Section: Methodsmentioning

confidence: 99%