Sperm Motile Efficiency (SME) of Fresh Spermatozoa in a Hypotonic Medium Correlates with their Cryosurvival in Fertile Men and Infertility Patients (Predicting Test)/Die Spermien-Motilitätseffizienz (SME) von Spermien in einem hypotonen Medium korreliert

Glander, H.‐J.; Bock, H.; Winiecki, P

doi:10.1111/j.1439-0272.1989.tb02425.x

Andrologia

2009

DOI: 10.1111/j.1439-0272.1989.tb02425.x

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Sperm Motile Efficiency (SME) of Fresh Spermatozoa in a Hypotonic Medium Correlates with their Cryosurvival in Fertile Men and Infertility Patients (Predicting Test)/Die Spermien-Motilitätseffizienz (SME) von Spermien in einem hypotonen Medium korreliert

H.‐J. Glander

H. Bock

P Winiecki

Abstract: Ein signifikanter Unterschied war sowohl hinsichtlich der SME nach Verdiinnung des Frischspermas mit 140 mOsm Losung als auch nach Kryokonservierung zwischen der fertilen Kontrollgruppe und der Gruppe der infertilen Patienten nachweisbar (Mann-Whitney-U-test). Die beiden Gruppen unterschieden sich in der Diskriminanzanalyse mit hochstem Signifikanzniveau in der SME unmittelbar nach Zugabe der hypoosmolaren Losung.

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“…S perm cryopreservation has been an important tool in assisted reproductive technology (ART). There have been many comprehensive reports on the cryopreservation of sperm in fertile and infertile men (Glander et al, 1989;Mossad et al, 1994;Tomlinson and Barratt, 1999). Furthermore, cryosurvival of washed and unwashed human sperm in normal and subnormal semen samples in the liquid phase of liquid nitrogen has also been reported (Keel and Webster, 1989;Hammadeh et al, 1999).…”

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Comparative Evaluation of Fresh and Washed Human Sperm Cryopreserved in Vapor and Liquid Phases of Liquid Nitrogen

SARITHA,

BONGSO

2001

Journal of Andrology

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Infectious organisms such as hepatitis B were recently shown to survive in liquid nitrogen. To prevent cross‐contamination of semen samples via liquid nitrogen, studies were undertaken to evaluate human sperm survival in the vapor phase of liquid nitrogen at −189°C. The study was conducted in 2 separate experiments. In the first experiment, a total of 30 unwashed, fresh semen samples (15 normozoospermic and 15 oligozoospermic) were evaluated for motility, vitality, and morphology after freeze‐thaw survival in vaporous (‐189°C) and liquid nitrogen (‐196°C; control) phases. Similar evaluations were carried out in a second experiment on 27 samples (15 normozoospermic and 12 oligozoospermic) that were previously washed by the swim‐up method. Motile sperm recovery rates were significantly different between liquid and vapor phases (unwashed, normozoospermic: 42.76% ± 3.23% vs 45.52% ± 4.44%, P < .05; washed, normozoospermic: 34.44% ± 4.41% vs 37.58% ± 3.90%, P < .05; unwashed, oligozoospermic: 16.53% ± 3.34% vs 18.25% ± 4.36%, P < .05; washed, oligozoospermic: 10.32% ± 2.54% vs 12.25% ± 2.81%, P < .05). Recovery rates for motility were much higher for unwashed samples compared with washed semen samples. In all experiments the recovery of normal and live forms showed no significant differences between the vapor and liquid nitrogen storage phases (P > .05). The results demonstrate that both washed and unwashed human sperm survive satisfactorily with good recovery in the vapor of liquid nitrogen and can be recommended for future storage in medically assisted conception programs.

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confidence: 99%

Comparative Evaluation of Fresh and Washed Human Sperm Cryopreserved in Vapor and Liquid Phases of Liquid Nitrogen

SARITHA,

BONGSO

2001

Journal of Andrology

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Evaluation of Human Sperm Function After Repeated Freezing and Thawing

BANDULARATNE,

BONGSO

2002

Journal of Andrology

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Sperm storage via freezing has been useful for men who have difficulty masturbating during assisted reproductive technology (ART) programs and before impotency caused by chemotherapy, vasectomy, and other procedures. Studies were undertaken to evaluate the extent of cryoinjury to sperm after repeated freezing and thawing. The results showed that normozoospermic and oligozoospermic sperm survived after 3 repeated freeze‐thaw cycles. The inclusion of seminal plasma did not seem to protect human sperm during freezing and thawing. There were no significant differences in recovery percentages for motile, vital, and morphologically normal sperm between slow and rapid freezing methods in thaws 1, 2, and 3 of normozoospermic and oligozoospermic unwashed (u), washed (w), and washed + seminal plasma (ws) samples. However, there were significant percentage drops in the recovery of motile and vital sperm between each thaw (ie, first to second thaw, and second to third thaw) using both slow and rapid freezing for u, w, and ws samples (P < .01). There were also no significant differences in percentage recovery of motile, vital, and morphologically normal sperm between u, w, and ws samples during thaws 1 to 3 in the normozoospermic and oligozoospermic groups. Sperm were capable of fertilizing hamster oocytes microinjected with single sperms after 3 freeze‐thaw cycles as evidenced by the formation of 2 distinct pronuclei and 2 polar bodies in 22.2% and 17.2% of normozoospermic and oligozoospermic samples, respectively. The numbers of normal vital motile sperm after 3 serial freeze‐thaw cycles are adequate for bringing about fertilization via intracytoplasmic sperm injection in ART programs. Thus, leftover washed sperm in laboratories that perform in vitro fertilization can be frozen, thawed, and refrozen several times without loss of the sperms' ability to fertilize. This approach has tremendous benefits for men who have difficulty producing sperm and for those with low and declining sperm counts.

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Correlation of the hypo‐osmotic swelling test, semen score and the zona‐free hamster egg penetration assay in humans

Okada¹,

Inomata²,

Matsuhashi³

et al. 1990

Int J of Andrology

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The correlation between the hypo-osmotic swelling test, the modified Eliasson score for human semen analysis and the hamster egg penetration assay was examined. The results showed a weak but significant correlation between the group of subjects with swelling rates below 50% and the above 80% group. These groups showed hamster egg penetration assay rates of 9 +/- 14% and 39 +/- 29%, respectively (P less than 0.035). A significant correlation was also found between modified Eliasson scores (17 +/- 10 and 5 +/- 7, respectively) and swelling rates below 50% and above 70% (P less than 0.001). These results suggest that spermatozoa showing a low swelling rate have a poor fertilizing capacity. Therefore, the swelling tests and analysis of the patients semen can be used to discriminate sperm quality.

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Sperm Motile Efficiency (SME) of Fresh Spermatozoa in a Hypotonic Medium Correlates with their Cryosurvival in Fertile Men and Infertility Patients (Predicting Test)/Die Spermien-Motilitätseffizienz (SME) von Spermien in einem hypotonen Medium korreliert

Cited by 4 publications

References 11 publications

Comparative Evaluation of Fresh and Washed Human Sperm Cryopreserved in Vapor and Liquid Phases of Liquid Nitrogen

Comparative Evaluation of Fresh and Washed Human Sperm Cryopreserved in Vapor and Liquid Phases of Liquid Nitrogen

Evaluation of Human Sperm Function After Repeated Freezing and Thawing

Correlation of the hypo‐osmotic swelling test, semen score and the zona‐free hamster egg penetration assay in humans

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