Sperm morphology, motility, and velocity in naturally occurring polyploid European weatherfish (Misgurnus fossilis L.)

Alavi, Sayyed Mohammad Hadi; Drozd, Bořek; Hatef, Azadeh; Flajšhans, Martin

doi:10.1016/j.theriogenology.2013.04.009

Cited by 10 publications

(13 citation statements)

References 38 publications

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“…In this study, diploid spermatozoa of natural tetraploid loaches M. anguillicaudatus exhibited active movement and sufficient duration of motility, which were not significantly different from those measured in haploid spermatozoa of diploids. Normal motility was also reported in diploid spermatozoa of natural tetraploid European weatherfish Misgurnus fossilis (Alavi et al, ). In the present study, head size and flagella length of diploid spermatozoa significantly increased according to the elevation of ploidy status from haploid to diploid, but general morphology did not differ between haploid and diploid spermatozoa, as also shown in rainbow trout Oncorhynchus mykiss (Chourrout et al, ), oyster Crassostrea gigas (Dong et al, ), and mussel Mytilus galloprovincialis (Komaru et al, ).…”

Section: Discussionmentioning

confidence: 70%

“…Deviation from the typical 9 þ 2 microtubule structure may result in non-motile flagella (Cosson, 2008). In contrast, Alavi et al (2013) reported active motility and no abnormal ultrastructures in the spermatozoa of natural triploid European weatherfish; the ploidy status of spermatozoa from triploid individuals was not determined in their study.…”

Section: Discussionmentioning

confidence: 80%

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Comparison of spermatozoa parameters, fine structures, and energy‐related factors among tetraploid, hyper‐tetraploid, and hyper‐triploid loaches (Misgurnus anguillicaudatus)

et al. 2014

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To evaluate the influence of ploidy elevation and aneuploidy on spermatozoa in the loach Misgurnus anguillicaudatus, we investigated some parameters (motility, concentration, and viability), fine structures (gross morphology, head size, and flagellum length), and energy-related biochemical factors (volume of mitochondrial mass per cell and ATP content) in diploid, hyper-diploid, and hexaploid-range spermatozoa produced in natural tetraploid, hyper-tetraploid, and hyper-triploid male loaches, respectively. Diploid spermatozoa exhibited vigorous movement and sufficient duration of motility similar to those in haploid spermatozoa. They had longer flagella, higher numbers and larger volume of mitochondria, and higher ATP content than haploid spermatozoa of wild-type diploids. No differences were observed in parameters and morphological characteristics between diploid and hyper-diploid spermatozoa. In contrast, the hexaploid-range spermatozoa of hyper-triploid males exhibited poor progressive motility in spite of a higher ATP content of spermatozoa. Spermatozoa with no flagella (36.0%) or multiple flagella (18.6%) were also observed in hyper-triploids. Ratios of head to flagellum length in hexaploid-range spermatozoa were significantly different from those of haploid spermatozoa. In addition to the normal 9+2 microtubule structure of the flagellum, an abnormal 9+1 microtubule structure was also observed in the spermatozoa of hyper-triploids.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 80%

Comparison of spermatozoa parameters, fine structures, and energy‐related factors among tetraploid, hyper‐tetraploid, and hyper‐triploid loaches (Misgurnus anguillicaudatus)

et al. 2014

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“…Even so, the significant differences in sperm trait values 283 Our study is especially novel in comparing sperm phenotypes across reproductive modes as well as 287 ploidy level, allowing us to at least in part decouple effects of polyploidy from those of asexuality. We 288 can draw parallels and contrasts with other ploidy-focused sperm studies: Alavi et al (2013) showed 289 that tetraploid European weatherfish (Misgurnus fossilis) produce sperm that are 4.8% larger than 290 sperm produced by their triploid counterparts, and Flajshans et al (2008) found that triploid male 291 Prussian carp (Carassius gibelio) make sperm with larger volume than sperm generated by diploid and 292 tetraploid males. Considered together along with these two earlier studies, we can use our results to 293 conclude that nuclear genome DNA content is not the sole driver of the patterns we observed.…”

Section: Among-and Within-species Variation In Sperm Morphology 256mentioning

confidence: 91%

“…We provided a separate control for nuclear DNA content, which can have major influences on sperm 138 phenotype (Alavi et al 2013…”

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confidence: 99%

Male evolution under relaxed selection: Evidence for degeneration in sperm produced by male snails from asexual lineages

Jalinsky

Logsdon

Neiman

2019

Preprint

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How drastic changes in selective regimes affect trait evolution is an important open biological question. We take advantage of naturally occurring and repeated transitions from sexual to asexual reproduction in a New Zealand freshwater snail species to address how relaxed selection on male-specific traits influences sperm morphology. The occasional production of male offspring by the otherwise all-female asexual lineages allows a unique and powerful opportunity to assess the fate of sperm traits in a context where males are unnecessary. These comparisons revealed that the sperm produced by “asexual” males are markedly distinct from sexual counterparts. In particular, the asexual male sperm harbored markedly higher phenotypic variation and was much more likely to be morphologically abnormal. Together, these data suggest that transitions to asexual reproduction might be irreversible at least in part because male function is likely to be compromised. More broadly, our results are consistent with a scenario where relaxed selection translates into rapid trait degeneration.

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“…To determine the sperm velocity, the distance of five head positions were measured and were divided into time spent for moving such distance. Velocity of approximately 50 to 60 motile sperm was measured for each individual [21] .…”

Section: Semen Evaluationmentioning

confidence: 99%

Karahindiba Ekstresi Yangılı ve Ağrılı Durumlarda Parasetamolün Alternatifi Olabilir mi? Biyokimyasal ve Reprodüktif Parametreler Yönünden Bir Değerlendirme

Ömür¹,

Yıldırım²,

Kandemir³

et al. 2016

Kafkas Univ Vet Fak Derg

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The aim of this study was to investigate the usage of Taraxacum officinale extract (TOE) in inflammatory and painful cases as an alternative to paracetamol (PRC) through the assessment of biochemical and reproductive parameters. Totally, 30 male Sprague Dawley rats aged eight weeks old, were used in this study. The animals were obtained from Atatürk University Experimental Research and Application Centre and kept under standard laboratory conditions. Commercial pellet chow and fresh drinking water were available ad libitum. Rats were divided into five groups: Group I (n= 6); referred as control. Group II (n=6); referred as TOE150 (150 mg/kg). Group III (n=6); referred as TOE200 (200 mg/kg). Group IV (n=6); referred as TOE250 (250 mg/kg). Group V (n=6); referred as Paracetamol (PRC) (2 g/kg). The treatment was performed for consecutive 8 days. The animals were tranquilized and sacrificed on 9th day of study. Blood samples, cauda epididymal semen samples and testes tissues were collected. Routine semen examinations were performed and oxidative stress levels of testicular tissues were assayed. Reproductive organ weights [total testes weight (TTW) and total cauda epididymal weights (TCEW)] were recorded. Motility in TOE250 group was significantly higher when compared to the other groups (P<0.05). Velocity of sperm cells in PRC group was significantly lower when compared to the other groups (P<0.05). Dead sperm rate in control group was significantly higher when compared to the other groups (P<0.001). On the other hand, the lowest TCEW was in TOE150 group (P<0.05). There were no differences in terms of TTW among all groups. Malondialdehyde (MDA) level of PRC group was significantly higher than the treatment groups (P<0.05). Besides, glutathione peroxidase (GPx) levels of PRC group were lower than the other groups (P<0.001). Superoxide dismutase (SOD) level of PRC group was significantly lower than the treatment groups (P<0.001). The lowest catalase (CAT) level was in PRC group and the highest glutathione (GSH) level was in T200 group (P<0.001). In conclusion, it was observed that TOE could use as alternative of PRC and hence can be avoided from negative effects of PRC on biochemical and reproductive parameters. Diğer gruplarla karşılaştırıldığında motilite TOE250 grubunda anlamlı olarak yüksekti (P<0.05). Diğer gruplarla karşılaştırıldığında velosite PRC grubunda anlamlı olarak düşüktü (P<0.05). Diğer gruplarla karşılaştırıldığında ölü sperm oranı kontrol grubunda anlamlı olarak yüksekti (P<0.001). Diğer taraftan en düşük total kauda epididimal ağırlık (TCEW), TOE150 grubundaydı (P<0.05). Bütün gruplar arasında total testis ağırlığı (TTW) açısından fark yoktu. Malondialdehit (MDA) düzeyi PRC grubunda tedavi gruplardan anlamlı olarak yüksekti (P<0.05). Bunun yanında, glutatyon peroksidaz (GPx) düzeyi PRC grubunda diğer gruplardan düşüktü (P<0.001). Superoksit dismutaz düzeyi (SOD) PRC grubunda tedavi gruplarına göre anlamlı olarak düşüktü (P<0.001). En düşük katalaz (CAT) düzeyi PRC grubunda ve en yüksek gluta...

show abstract

Sperm morphology, motility, and velocity in naturally occurring polyploid European weatherfish (Misgurnus fossilis L.)

Cited by 10 publications

References 38 publications

Comparison of spermatozoa parameters, fine structures, and energy‐related factors among tetraploid, hyper‐tetraploid, and hyper‐triploid loaches (Misgurnus anguillicaudatus)

Comparison of spermatozoa parameters, fine structures, and energy‐related factors among tetraploid, hyper‐tetraploid, and hyper‐triploid loaches (Misgurnus anguillicaudatus)

Male evolution under relaxed selection: Evidence for degeneration in sperm produced by male snails from asexual lineages

Karahindiba Ekstresi Yangılı ve Ağrılı Durumlarda Parasetamolün Alternatifi Olabilir mi? Biyokimyasal ve Reprodüktif Parametreler Yönünden Bir Değerlendirme

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