Sperm morphology and in vitro fertilization outcome: a direct comparison of World Health Organization and strict criteria methodologies

Morgentaler, Abraham; Fung, M.Y.; Harris, Doria; Powers, Robert D.; Alper, Michael M.

doi:10.1016/s0015-0282(16)57981-3

Cited by 39 publications

(21 citation statements)

References 14 publications

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“…Of these, sperm morphology is much emphasized recently and is proven to be one of the important predictors in determining the fertilizing capacity of sperm both in vivo and in vitro conditions [23][24][25]. Several clinical studies have demonstrated a clear association between sperm head morphology and improvement in IVF success rate [4,26,27]. However, other works showed lack of correlation between these parameters [28,29].…”

Section: Discussionmentioning

confidence: 99%

“…It is primarily evaluated by routine semen analysis which assesses the sperm count, motility and morphological classification [1]. Various morphological abnormalities of sperm head and morphometric parameters are often associated with infertility and to a large extent influence the fertilization cates and pregnancy outcome [2][3][4]. Traditionally, these parameters were routinely evaluated by light microscopy, but the drawbacks of this methodology such as subjectivity and high interobserver variability makes interpretation unreliable [5].…”

Section: Introductionmentioning

confidence: 99%

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CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

Sivanarayana

Krishna

Prakash³

et al. 2012

J Assist Reprod Genet

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Purpose The present study was undertaken to evaluate the effects of morphokinetic abnormalities of human spermatozoa on chromatin packing and DNA integrity and possible beneficial effects of sperm selection in ICSI. Methods Semen samples from 1002 patients were analysed for morphology and motility using CASA. Protamine status and DNA fragmentation were analysed by chromomycin A3 staining and sperm chromatin dispersion assay respectively. Results Sperms with elongated, thin, round, pyri, amorphous, micro and macro forms were significantly higher in teratozoospermic and oligoasthenoteratozoospermic groups. Significant difference in chromatin packing and DNA fragmentation index was observed in these abnormal groups compared with normal. Similarly significant correlation was also seen between abnormal motility parameters and DNA fragmentation index in asthenozoospermic group compared with normal. Conclusions Specific abnormal morphological forms have higher incidence of chromatin packing abnormalities and DNA fragmentation. Using these sperms in ICSI might have an impact on fertilization, embryo development and abortion rates. These can be selectively avoided during ICSI procedure to improve ART outcome.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

Sivanarayana

Krishna

Prakash³

et al. 2012

J Assist Reprod Genet

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“…Such illusions are a reality and can influence our views also on micrographs. Figure 3 contains the morphological data taken from Appendix Table 1.2 in the 5th WHO manual [6], data from my own laboratory as they were in the early 1980s, and information from Morgentaler et al [16]. The shaded areas represent the 95% CI when calculated according to Altman et al [14] and under the assumption that all assessments are fully dichotomised.…”

Section: Morphology Of Spermatozoamentioning

confidence: 99%

“…The lines referring to Morgentaler et al [16] are constructed from data in their publication and refer to the assessment according to 'strict criteria' or 'traditional morphology' (WHO 1987), respectively. The conclusion drawn by Morgentaler et al [16] was that 'comparison of traditional morphology and strict criteria with regard to in vitro fertilization (IVF) outcome favored traditional morphology in several areas.…”

Section: Morphology Of Spermatozoamentioning

confidence: 99%

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Semen analysis with regard to sperm number, sperm morphology and functional aspects

Eliasson¹

2010

Asian J Androl

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The new World Health Organization (WHO) Manual for Semen Analysis contains several improvements. One is that the 20 million spermatozoa per mL paradigm has been ousted in favour of proper calculations of lower reference limits for semen from men, whose partners had a time-to-pregnancy of 12 months or less. The recommendation to grade the progressive motility as described in the third and fourth editions of the WHO manual was not evidence-based, and WHO was therefore motivated to abandon it. However, the new recommendation is not evidence-based either, and it is difficult to understand the rational for the new assessment. It may have been a compromise to avoid returning to the rather robust system recommended in the first edition (1980). The unconditional recommendation of the 'Tygerberg strict criteria' is not evidence-based, and seems to be the result of an unfortunate bias in the composition of the Committee in favour of individuals known to support the 'strict criteria' method. This recommendation will have negative effects on the development of andrology as a scientific field. Given the importance of the WHO manual, it is unfortunate that the recommendations for such important variables, as motility and morphology, lack evidence-based support.

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Sperm Morphology Assessment—Historical Perspectives and Current Opinions

MORTIMER,

MENKVELD

2001

Journal of Andrology

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Sperm morphology and in vitro fertilization outcome: a direct comparison of World Health Organization and strict criteria methodologies

Cited by 39 publications

References 14 publications

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

Semen analysis with regard to sperm number, sperm morphology and functional aspects

Sperm Morphology Assessment—Historical Perspectives and Current Opinions

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