Sperm migration through the uterine cervix in the macaque during the menstrual cycle

Jaszczak, S.; Hafez, E. S. E.

doi:10.1016/0002-9378(73)90556-5

Cited by 9 publications

(2 citation statements)

References 18 publications

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“…In addition, cryo¬ preservation of macaque spermatozoa may provide a useful model system for investigating the nature and consequences of cryodamage to human spermatozoa. The cynomolgus monkey (Macaca fascicularis), whose male and female counterparts are reproductively similar to humans (Jaszczak & Hafez, 1973;), is easily maintained in captivity and can serve as a model species.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis)

Tollner¹,

VandeVoort²,

Overstreet³

et al. 1990

Reproduction

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Three egg-yolk diluents, which have been used successfully in cryopreservation of human spermatozoa, were compared for their ability to protect macaque semen against cryodamage. TEST (Tes + Tris + egg yolk), TEST with 20% skim milk (TSM), and egg yolk-citrate (EYC), each with 3 or 5% glycerol were compared using 12 ejaculates from 6 male cynomolgus macaques. Computer-aided analysis of sperm motion was used to determine the percentage motility (%M), curvilinear velocity (VCL), and linearity (LIN) of spermatozoa after thawing. The supravital stain Hoechst 33258 and a fluoresceinated pea lectin were used to determine the % of viable spermatozoa with intact acrosomes. TSM and TEST were superior to EYC in terms of % M and of % viable, acrosome-intact spermatozoa. TSM and TEST produced equivalent VCL and LIN values, while EYC had clearly reduced VCL and LIN. There were no interactions between diluent and glycerol level. The 3% glycerol level gave superior results to 5% glycerol for %M. EYC, which is widely used for cryopreservation of human spermatozoa, was not suitable for cynomolgus monkey semen. Artificial insemination with semen cryopreserved in TSM resulted in a healthy, full-term infant.

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Section: Introductionmentioning

confidence: 99%

Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis)

Tollner¹,

VandeVoort²,

Overstreet³

et al. 1990

Reproduction

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“…4a). In Primates near ovulation, most spermatozoa have been described as densely crowded in mucus strands parallel to the canal axis before entering the crypts (37,38). Owing to the short phylogenetical distance, such a mechanism of temporary storage could operate also in women.…”

Section: Discussionmentioning

confidence: 99%

Involvement of the glycoproteic meshwork of cervical mucus in the mechanism of sperm orientation

Chretien

2003

Acta Obstet Gynecol Scand

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Movement of cynomolgus and rhesus monkey spermatozoa collected from the lower female reproductive tract

et al. 1989

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Postcoital (pc) cervical mucus was collected in 73 menstrual cycles of cynomolgus monkeys and in 43 cycles of rhesus monkeys at 2, 6, 10, 30 hr pc. Videomicrography was used to analyze sperm numbers and movement in the mucus. Both cynomolgus and rhesus monkeys had comparable populations of motile sperm in the mucus at 2 hr pc. However, by 6 hr pc, cervical mucus from cynomolgus monkeys contained twice as many total sperm and motile sperm as mucus from rhesus monkeys (P less than .05). Mean swimming speeds of the free-swimming cervical sperm were similar for the two species at this time. No motile sperm were recovered in mucus from rhesus monkeys at 30 hr pc. In cynomolgus monkeys, however, 14 of the 26 animals examined at 30 hr pc had motile sperm in their mucus. These sperm exhibited lower percent motility, percent free-swimming sperm, and swimming speed than those sperm observed at 6 hr pc. Uterine sperm were collected by transcervical or transuterine aspiration from cynomolgus monkeys. In the transcervical technique, sperm were successfully obtained in four of nine animals examined at 6 hr and in four of five animals at 30 hr pc. The percentage of motile sperm in the uterine fluid was high, 82% +/- 4%, and the swimming speeds (86 +/- 2 microns/sec) were higher than those observed in cervical mucus. Approximately 5-10% of the uterine sperm exhibited swimming motions similar to the hyperactivated motility seen in most mammals. These findings indicate that the sperm cervical mucus interaction in vivo in cynomolgus monkeys has more similarities to the human situation than does the interaction in rhesus monkeys.

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Sperm migration through the uterine cervix in the macaque during the menstrual cycle

Cited by 9 publications

References 18 publications

Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis)

Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis)

Involvement of the glycoproteic meshwork of cervical mucus in the mechanism of sperm orientation

Movement of cynomolgus and rhesus monkey spermatozoa collected from the lower female reproductive tract

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