Sperm membrane proteins associated with the boar semen cryopreservation

Guimarães, Daianny Barboza; Barros, Tatyane Bandeira; Tilburg, Maurício Fraga van; Martins, Jorge André Matias; Moura, Arlindo A.; Moreno, Frederico Bruno Mendes Batista; Monteiro-Moreira, Ana Cristina de Oliveira; Moreira, Renato A.; Toniolli, Ricardo

doi:10.1016/j.anireprosci.2017.06.005

Cited by 30 publications

(16 citation statements)

References 57 publications

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“…The results were, to the best of our knowledge, the first reporting differences in protein composition between boar spermatozoa of different ejaculate fractions/portions, and they highlighted a putative relevance of such differences on the ability of sperm to successful sustaining cryopreservation. Previous studies demonstrated quantitative differences in some specific sperm proteins between boar semen ejaculates showing good and bad sperm freezability (Vilagran et al 2013(Vilagran et al , 2014Guimaraes et al 2017). Furthermore, Chen et al (2014) demonstrated changes in sperm proteome during the cryopreservation process, suggesting that sperm proteins may play an important role for cryosurvival.…”

Section: Moreovermentioning

confidence: 98%

Is boar sperm freezability more intrinsically linked to spermatozoa than to the surrounding seminal plasma?

Roca

Pérez-Patiño

et al. 2018

Animal Reproduction Science

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This study aimed to elucidate the effect of seminal plasma (SP) from post-SRF on boar sperm freezability and, in addition, to determine the relevance of sperm itself to sustain cryopreservation, regardless of the SP surrounding them. Twelve ejaculates from three boars were manually collected in fractions/portions, P1: the first 10 mL of the SRF, P2: the rest of the SRF and the post-SRF. Immediately, samples were centrifuged to separate spermatozoa from the surrounding SP. Spermatozoa from P1 and P2 were then incubated with its own SP or that from post-SRF, diluted in BTS (1:1, v/v) at 17 °C overnight before being frozen in 0.5 mL straws using a standard protocol. Sperm motility (total and progressive) deteriorated (P < 0.05) when P1- or P2-sperm when incubated overnight in SP from post-SRF, while sperm viability differed between P1 and P2 (P < 0.05) regardless of the SP they were incubated in. Post-thaw sperm quality and functionality differed between P1 and P2, regardless of the SP used for overnight pre-freezing incubation. Post-thaw motility (P < 0.05) and viability (P < 0.01), as well as plasma membrane fluidity (P < 0.05) or lipid peroxidation values (P < 0.01) were best in P1 sperm compared to those of P2. The protein profile of sperm from P1 and P2, analyzed by 2D-PAGE, showed qualitative differences, which suggest that sperm rather than SP would explain differences in sperm freezability between ejaculate fractions/portions. Use of P1 fraction spermatozoa seems thus optimal for cryopreservation.

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Section: Moreovermentioning

confidence: 98%

Is boar sperm freezability more intrinsically linked to spermatozoa than to the surrounding seminal plasma?

Roca

Pérez-Patiño

et al. 2018

Animal Reproduction Science

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“…This proteomic mapping of boar seminal plasma contributes a protein library with biomarker potential (Perez‐Patiño et al, ). The membrane proteins, F‐actin capping protein subunit alpha 1 and arylsulfatase A, are expressed more in the sperm membrane of boars with greater motility and vigour after thawing and could serve as markers of post‐thaw kinetic parameters (Guimarães et al, ).…”

Section: Freezability Markersmentioning

confidence: 99%

“…The membrane proteins, F-actin capping protein subunit alpha 1 and arylsulfatase A, are expressed more in the sperm membrane of boars with greater motility and vigour after thawing and could serve as markers of post-thaw kinetic parameters (Guimarães et al, 2017).…”

Section: Free Z Ab Ilit Y Mark Er Smentioning

confidence: 99%

“…For this reason, frozen-thawed boar semen has not been widely accepted by the pork industry due to the greater costs of the semen and reduced fertility (Grossfeld et al, 2008). Hence, efforts are focused on achieving more satisfactory results, or modifying the existing protocols, by adding cholesterol (Tomás, Gómez-Fernández, Gómez-Izquierdo, Mocé, & de Mercado, 2014), seminal plasma (Torres et al, 2016), antioxidants (Estrada, Rodríguez-Gil, Rivera Del Álamo, Peña, & Yeste, 2017;Qian et al, 2016), identifying freezability markers in reproductive males (Casas et al, 2009;Guimarães et al, 2017) and so forth. The aim of this article is to provide the reader with background on boar semen preservation methods (storage in liquid, slow and fast freezing), the effects of cryopreservation on boar sperm, and updated information about the advances and the impact of these on fertilization rates, which is the best predictor of the quality of a frozen-thawed semen sample.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preservation of boar semen: An update

Pezo¹,

Romero

Zambrano³

et al. 2019

Reprod Domestic Animals

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Contents In the pork industry, artificial insemination and the storage of boar semen in liquid at 17°C are routinely applied to optimize the ejaculate and bring about rapid genetic changes that are reflected in the animal protein. Although the results are satisfactory, they are below what occurs with natural mating. It is currently possible to preserve boar semen with storage at 17°C and slow freezing, since to date there is only one study on vitrification, with negative results applicable only in the case of implementing an intracytoplasmic sperm injection. In both methods and due to the sensitivity of boar sperm to osmotic and temperature changes, there is a loss in the quality of the initial sample; however, slow freezing in boar semen has greater deleterious effects on the sample that are reflected in the pregnancy rates and number of live births. Therefore, only 1% of all inseminations are done with frozen semen. The aim of this review is to provide advances and results of studies conducted on the preservation of boar semen, delving more deeply into the critical points that each of the preservation techniques presents, including bacterial contamination, extender components, temperature, ice nucleation, use of additives in extenders and the main deleterious effects on sperm quality.

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“…Moreover, sperm proteomic also allows identifying the cryopreservation ability of semen within species. For instance, in boars, sperm membrane proteins can be used as markers for semen cryopreservation [25].…”

Section: Proteomics In the Reproduction Fieldmentioning

confidence: 99%

Application of Some Proteome Analysis Techniques in Animal Reproduction

Baykalir¹,

Baykalır²,

Şimşek³

2018

New Insights Into Theriogenology

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This chapter focuses on the application of proteome analysis techniques to animal reproduction and provides general information on one-dimensional (1D) and two-dimensional (2D) electrophoresis, chromatographic methods, and mass spectrometer (MS), widely used in proteomics studies. Proteome consists of an entire complement of proteins expressed by a cell, tissue, or organism. Proteomics reveals functions of proteins encoded in the genome. These functions include posttranslational modifications (PTMs) and alterations in the protein synthesis. Animal reproduction takes a key role in livestock, and increasing the reproduction rate in flocks plays an important role in livestock management. Studying the proteins related to reproduction could guide on how to increase fertility. Recent studies addressed the proteome constitution of both male and female reproductive system. Follicular fluid, endometrium, and ovary proteins were analyzed in females by proteomics study, while in males, sperm proteomics was more focused. Information obtained on this issue is also beneficial for the development of reproductive technologies such as in vitro fertilization and embryo transfer. Strategies to increase fertility in animals can be revealed by proteomic studies, and a more profound knowledge on proteomics may become helpful to develop and enhance the efficiency of reproductive technologies.

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Sperm membrane proteins associated with the boar semen cryopreservation

Cited by 30 publications

References 57 publications

Is boar sperm freezability more intrinsically linked to spermatozoa than to the surrounding seminal plasma?

Is boar sperm freezability more intrinsically linked to spermatozoa than to the surrounding seminal plasma?

Preservation of boar semen: An update

Application of Some Proteome Analysis Techniques in Animal Reproduction

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