Sperm maturation screening and the effect of ecdysone on sperm development of silkworm Bombyx mori

Cheng, Hao; Wang, Yanyan; Chang, Mei‐Ling; Zhang, Kun; Cai, Zhiyan; Qian, Yuxin; Li, Dandan

doi:10.1016/j.aspen.2022.101916

Cited by 2 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If the absence of JH were all that mattered, then transplanting testes into organ culture without the hormone ought to be sufficient to initiate apyrene divisions, but this does not happen. In organ culture experiments using testes from C. pomonella ( 46 ) as well as B. mori ( 65 ), hemolymph is indispensable for proper spermatogenesis of either type, but addition of hemolymph that is predicted to contain ASIF is unable to change the developmental fate of cysts explanted into culture, and whether a cyst produces eupyrene or apyrene sperm depends only on the age and developmental progress of the donor of the cyst ( 66 ).…”

Section: Lepidopteran Spermatogenesismentioning

confidence: 99%

Dichotomous sperm in Lepidopteran insects: a biorational target for pest management

Seth,

Yadav,

Reynolds

2023

Front. Insect Sci.

View full text Add to dashboard Cite

Lepidoptera are unusual in possessing two distinct kinds of sperm, regular nucleated (eupyrene) sperm and anucleate (apyrene) sperm (‘parasperm’). Sperm of both types are transferred to the female and are required for male fertility. Apyrene sperm play ‘helper’ roles, assisting eupyrene sperm to gain access to unfertilized eggs and influencing the reproductive behavior of mated female moths. Sperm development and behavior are promising targets for environmentally safer, target-specific biorational control strategies in lepidopteran pest insects. Sperm dimorphism provides a wide window in which to manipulate sperm functionality and dynamics, thereby impairing the reproductive fitness of pest species. Opportunities to interfere with spermatozoa are available not only while sperm are still in the male (before copulation), but also in the female (after copulation, when sperm are still in the male-provided spermatophore, or during storage in the female’s spermatheca). Biomolecular technologies like RNAi, miRNAs and CRISPR-Cas9 are promising strategies to achieve lepidopteran pest control by targeting genes directly or indirectly involved in dichotomous sperm production, function, or persistence.

show abstract

Section: Lepidopteran Spermatogenesismentioning

confidence: 99%

Dichotomous sperm in Lepidopteran insects: a biorational target for pest management

Seth,

Yadav,

Reynolds

2023

Front. Insect Sci.

View full text Add to dashboard Cite

show abstract

“…After hatching larvae were fed a diet of fresh mulberry leaves collected in the wild and feeding boxes were changed regularly. All tissue samples were obtained from fifth instar larvae, a developmental stage that contains eupyrene spermatocytes and sperm bundles ( Cheng et al 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…However, apyrene spermatogenesis, which is regulated by Sex-lethal ( Sakai et al 2019 ; Chen et al 2020 ), does not begin until the second day of prepupation, after which production of eupyrene sperm ceases. By adulthood, 80% of the sperm bundles are apyrene ( Cheng et al . 2022 ).…”

Section: Introductionmentioning

confidence: 99%

A molecular cell biology toolkit for the study of meiosis in the silkworm Bombyx mori

Xiang

Tsuchiya

Guo

et al. 2023

G3: Genes, Genomes, Genetics

View full text Add to dashboard Cite

Meiosis is usually described as four essential and sequential processes: (1) homolog pairing; (2) synapsis, mediated by the synaptonemal complex (SC); (3) crossing over; and (4) segregation. In this canonical model, the maturation of crossovers into chiasmata plays a vital role in holding homologs together and ensuring their segregation at the first meiotic division. However, Lepidoptera (moths and butterflies) undergo three distinct meiotic processes, only one of which is canonical. Lepidoptera males utilize two meiotic processes: canonical meiosis that produces nucleated fertile sperm, and a noncanonical meiosis that produces anucleated nonfertile sperm which are nonetheless essential for reproduction. Lepidoptera females, which carry heteromorphic sex chromosomes, undergo a completely achiasmate (lacking crossovers) meiosis, thereby requiring an alternative mechanism to ensure proper homolog segregation. Here we report the development of a molecular cell biology toolkit designed to properly analyze features of meiosis, including the SC structure and function, in the silkworm Bombyx mori. In addition to standard homology searches to identify Bombyx orthologs of known SC encoding genes, we developed an ortholog discovery app (Shinyapp) to identify Bombyx orthologs of proteins involved in several meiotic processes. We used this information to clone genes expressed in the testes and then created antibodies against their protein products. We used the antibodies to confirm the localization of these proteins in normal male spermatocytes, as well as using in vitro assays to confirm orthologous interactions. The development of this toolkit will facilitate further study of the unique meiotic processes that characterize meiosis in Lepidoptera.

show abstract

Sperm maturation screening and the effect of ecdysone on sperm development of silkworm Bombyx mori

Cited by 2 publications

References 24 publications

Dichotomous sperm in Lepidopteran insects: a biorational target for pest management

Dichotomous sperm in Lepidopteran insects: a biorational target for pest management

A molecular cell biology toolkit for the study of meiosis in the silkworm Bombyx mori

Contact Info

Product

Resources

About