Sperm immobilized before intracytoplasmic sperm injection undergo ultrastructural damage and acrosomal disruption

Gómez‐Torres, María José; Ten, Jorge; Girela, José L.; Romero, Jesús; Bernabéu, Rafael; Juan, J. De

doi:10.1016/j.fertnstert.2006.12.063

Cited by 17 publications

(10 citation statements)

References 4 publications

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“…There are reports of SEM observations of the mammalian mature sperm, including the human sperm (Downing Meisner et al, ; Gómez‐Torres et al, ; Moretti and Collodel, ). In Drosophila , however, mature sperm have not generally been studied by SEM, and the morphological features of the sperm surface have not been described previously.…”

Section: Discussionmentioning

confidence: 99%

Electron microscopic observation of the sagittal structure ofDrosophilamature sperm

Yasuno

Yamamoto

2014

Microsc. Res. Tech.

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Observation of sperm development and determination of their morphological characteristics are very important to the understanding of phylogenetic relationships and the study of sperm function during fertilization. Although ultrastructural studies of sperm development in the testes of the fruit fly Drosophila have been performed, there are few reports describing electron microscopic morphology of mature sperm, that is, those released from the testes to the seminal vesicles. Here, we present the first report of the sagittal organization of Drosophila sperm head and neck regions by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The head and tail structures of a mature sperm, for example, the acrosome, nucleus, and flagellum, were easy to distinguish by the morphological characteristics of the sperm surface by SEM. The morphological relationships between the surface and internal structures of mature sperm were confirmed by observing longitudinal sections with TEM. Our approach overcame the technical difficulties involved in sample preparation for electron microscopic observation of the Drosophila mature sperm head, and therefore, this study serves as an important foundation for future genetic dissection of sperm ultrastructure and function in male sterile mutants.

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Section: Discussionmentioning

confidence: 99%

Electron microscopic observation of the sagittal structure ofDrosophilamature sperm

Yasuno

Yamamoto

2014

Microsc. Res. Tech.

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“…This may potentially increase the risk of in situ sperm DNA fragmentation and its consequences on the reproductive outcome. It has been shown that sperm immobilization before ICSI is one of the causes of sperm ultrastructural damages (13). However, we did not perform mechanical sperm immobilization in our study.…”

Section: Discussionmentioning

confidence: 85%

Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in polyvinylpyrrolidone-based sperm-immobilization medium

Salian

Kalthur

Uppangala

et al. 2012

Fertility and Sterility

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“…Complete or incomplete separation of the sperm head‐neck or head‐midpiece is one of severe structural aberrations. Previous studies showed that infertile men with certain diseases were associated with separation of the sperm head‐neck (Chemes et al., ; Courtot, Nikas, Gravanis, & Psychoyos, ; Gómez‐Torres et al., ). On the other hand, it is worth notice that such a separation may be a morphological artefact based on the following analyses: (i) the sperm head is a flattened ovoid.…”

Section: Discussionmentioning

confidence: 99%

Preparation and observation methods can produce misleading artefacts in human sperm ultrastructural morphology

Zhu

2018

Andrologia

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The aim of this study was to evaluate the production of artefacts during preparation and observation of human sperm for ultrastructural morphology and analyse the possible reasons of causing these artefacts. Under the scanning electron microscopy, damaged sperm heads (crack or/and rupture), necks (head-neck or head-midpiece separation) and midpiece (disassembled and denuded axoneme ultrastructures, bent midpiece) were analysed to be the consequence of exogenous effects. Thirty infertile men with teratozoospermia revealed more spermatozoa with damage to head, neck and midpiece than did thirty fertile males (p < .01). After the samples from fertile males underwent five repeated observations, most sperm heads and necks in the samples were destroyed when compared with the single observation (p < .01). Destroyed sperm heads were full of cracks and peelings, even sperm tails were broken and fragmented, and separations of the sperm head-neck or head-midpiece became common. Spermatozoa from fertile males with centrifugation of 600 g for washing sperm exhibited more damage to the midpiece than those with the 300 g (p < .01). These results demonstrate that preparation and observation methods can damage sperm ultrastructures, leading to producing artefacts of ultrastructural morphology. The artefacts of sperm ultrastructural morphology may be associated with sperm structural fragility, preparation conditions and electron beam damage.

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Sperm immobilized before intracytoplasmic sperm injection undergo ultrastructural damage and acrosomal disruption

Cited by 17 publications

References 4 publications

Electron microscopic observation of the sagittal structure ofDrosophilamature sperm

Electron microscopic observation of the sagittal structure ofDrosophilamature sperm

Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in polyvinylpyrrolidone-based sperm-immobilization medium

Preparation and observation methods can produce misleading artefacts in human sperm ultrastructural morphology

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