Sperm flow cytometric parameters, antioxidant status, and testicular histomorphology in roosters fed diets supplemented with camphor

Raei, Hamid; Torshizi, Mohammad Amir Karimi; Sharafi, Mohsen; Ahmadi, Hamed

doi:10.1016/j.psj.2022.102014

Cited by 2 publications

(3 citation statements)

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“…MDA is the main by-product of lipid peroxidation, and its assessment can provide information regarding the peroxidative damage in sperm (Raei et al, b, 2021. Because spermatozoa cells are unable to synthesize PUFA or repair damages, protection against peroxidative damage would be an important mechanism for maintaining PUFA in sperm (Raei et al, 2022 b;Behnamifar et al, 2021). At the end of the experiment, a 5.25-fold decrease in MDA content of F2G3 group was found in comparison to F2G0 group.…”

Section: Discussionmentioning

confidence: 93%

“…In the present study, the testicular weight was not affected by the experimental groups. However, it is demonstrated that roosters fed on a diet supplemented with antioxidants present high testicular weights which is positively correlated with semen quality (Raei et al, 2022 b;Golzar Adabi et al, 2022). Reportedly, testicular weight affects sperm's health and production (Sun et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

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Sperm fatty acid composition, semen quality, and reproductive performance of roosters fed diets supplemented with n-3 fatty acids and ginger (Zingiber officinale)

Demir

Raei

Adabi

et al. 2023

Annals of Animal Science

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This study aimed to investigate the effect of dietary n-3 fatty acid and ginger (Zingiber officinale) supplementation on semen quality, sperm fatty acids, and reproductive performance of roosters. Seventy-two roosters (30 weeks old) were randomly allocated into 4 dietary treatments including 1) basal diet as the control group (F0G0), 2) basal diet supplemented with 20 g/kg fish oil (F2G0), 3) basal diet supplemented with 30 g/kg ginger powder (F0G3), and 4) basal diet supplemented with 20 g/kg fish oil and 30 g/kg ginger powder (F2G3) for 10 consecutive weeks. The levels of sperm linolenic, EPA, and DHA were higher in fish oil-fed roosters (P<0.05). A lower percentage of sperm linoleic and arachidonic acids were recorded in F2G0 and F2G3 groups (P<0.05). At 38 weeks of age, a higher sperm volume was found in F0G3 roosters than in the F0G0 and F2G0 groups. From week 34 to the end of the experiment, sperm viability, sperm abnormality, and sperm motility were significantly improved in the GP-fed roosters (F0G3 and F2G3 groups) (P<0.05). Significantly lowest sperm concentration overall the experiment was observed in the F2G0 group (P<0.05). Higher testosterone levels and lower malondialdehyde (MDA) content were recorded in F0G3 and F2G3 groups in comparison to the F2G0 (P<0.05). Also, the fertility rate of collected eggs from F0G3 and F2G3 groups was higher compared to F2G0 group (P<0.05). In conclusion, although the use of fish oil in roosters’ diets alone had a negative effect on some parameters related to reproductive performance, the use of ginger powder alone or along with fish oil improved semen quality and fertility potential.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Sperm fatty acid composition, semen quality, and reproductive performance of roosters fed diets supplemented with n-3 fatty acids and ginger (Zingiber officinale)

Demir

Raei

Adabi

et al. 2023

Annals of Animal Science

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“…In the current investigation, rutin supplementation of ram sperm not only increased mitochondrial activity, but also inhibited apoptosis. The mitochondria are not only the cell energy source but also an essential part of the process that controls apoptosis 27 . Our result suggested that rutin may improve sperm motility and viability by enhancing mitochondrial function.…”

Section: Discussionmentioning

confidence: 99%

Enhancing post-thaw quality of ram epididymal sperm by supplementation of rutin in cryopreservation extender

Najafi

Mohammadi

Sharifi

2023

Sci Rep

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The purpose of this study was to examine the effect of different rutin concentrations on rams epididymal sperm. A local slaughterhouse provided 50 pair of testes from 25 rams. The testes were sent to the lab at room temperature. Spermatozoa were extracted by suspending portions of cauda epididymis in tris solution. Ram sperm was cryopreserved (in liquid nitrogen) in a tris extender containing rutin at 0, 0.5, 0.75, 1, and 1.25 mM. Rutin showed superior sperm total and progressive motility, beat cross frequency, straight line velocity, velocity average pathway and membrane integrity values at 0.75 and 1 mM. The morphology of the sperm and the superoxide dismutase levels did not significantly change with different treatments. Moreover, rutin at 0.75 and 1 mM was also shown to have the highest level of mitochondrial activity. The results showed ATP, total antioxidant capacity, and glutathione peroxidase levels were significantly greater in the rutin 0.75 and 1 mM groups (P < 0.05). Rutin at 0.75 and 1 mM levels had the lowest reactive oxygen species concentrations. Rutin at 0.75 and 1 mM substantially increased the proportion of viable sperm (P < 0.05). The lowest amount of apoptosis was observed in 0.75 and 1 mM rutin. Rutin at 0.75 and 1 mM yielded the least significant percentage of dead sperm. It may be inferred that adding 0.75 and 1 mM to the sperm extender can enhance the quality of the epididymal sperm in rams after the cryopreservation process.

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Sperm flow cytometric parameters, antioxidant status, and testicular histomorphology in roosters fed diets supplemented with camphor

Cited by 2 publications

References 56 publications

Sperm fatty acid composition, semen quality, and reproductive performance of roosters fed diets supplemented with n-3 fatty acids and ginger (Zingiber officinale)

Sperm fatty acid composition, semen quality, and reproductive performance of roosters fed diets supplemented with n-3 fatty acids and ginger (Zingiber officinale)

Enhancing post-thaw quality of ram epididymal sperm by supplementation of rutin in cryopreservation extender

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