Sperm DNA Fragmentation: Mechanisms of Origin

Muratori, Monica; Marchiani, Sara; Tamburrino, Lara; Baldi, Elisabetta

doi:10.1007/978-3-030-21664-1_5

Cited by 62 publications

(54 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Finally, FSH is prescribed together with hCG or nutraceuticals only in 15.1% of men treated. Antioxidants seem useful to reduce sperm DNA fragmentation index (sDF), potentially improving the FSH beneficial action on spermatogenesis . Hypothetically, the combined treatment could act both on the first phases of spermatogenesis with FSH (ie on spermatogonia) and on the last developmental steps of spermatocytes with the antioxidant action, but this requires experimental demonstration in clinical trials and this survey did not provide useful data here for.…”

Section: Discussionmentioning

confidence: 95%

Use of follicle‐stimulating hormone for the male partner of idiopathic infertile couples in Italy: Results from a multicentre, observational, clinical practice survey

et al. 2020

View full text Add to dashboard Cite

Background:The management of male idiopathic infertility is heterogeneous.Although meta-analyses reported the effectiveness on pregnancy rate, the real clinical impact of follicle-stimulating hormone (FSH) was not evaluated so far. In Italy, FSH is approved by the National Medicines Agency (AIFA) for idiopathic infertile patients with FSH < 8 IU/L, independently of semen parameters.Aim: Primary endpoint was to record the therapeutic approach to the male partner of infertile couples. Secondary aim was to assess changes of semen parameters during FSH treatment. Methods:A multicentre, prospective, observational, clinical practice survey was carried out, enrolling the male partner of infertile couples attending ten Italian participating centres. Inclusion criteria were as follows: couple infertility, age >18 years and FSH serum levels <8 IU/L. Thus, all men in which AIFA allowed the FSH prescription were enrolled. Primary endpoint was the number of infertile patients treated with FSH. Secondary outcomes were semen parameters. The treating physician decided whether to offer FSH therapy and whether to re-evaluate the male partner. Results:A total of 718 infertile couples were enrolled, and 241 patients were reevaluated (median follow-up: 4.5 months). In 64.9% (466 patients), a treatment was prescribed. FSH was prescribed in 397 patients (85.2% of treated men). Sperm concentration (P = .002) and normal form percentage (P < .001) significantly improved during FSH administration. No correlation was found between these parameters and FSH duration (P = .545 and P = .627, respectively) or dosage (P = .455 and P = .533, respectively). Among patients treated with FSH, the incidence of oligozoospermia decreased from 73.0% to 56.0% (P < .001) and teratozoospermia from 43.6% to 27.7% (P < .001).Discussion: This first nation-wide survey reveals a FSH prescription rate of 55% in patients qualifying for treatment according to AIFA. Although the study was not designed to highlight FSH efficacy in male infertility, a slight increase in semen parameters was demonstrated in about half of the treated men without adverse events. K E Y W O R D Sfollicle-stimulating hormone, FSH, male idiopathic infertility, open registry

show abstract

Section: Discussionmentioning

confidence: 95%

Use of follicle‐stimulating hormone for the male partner of idiopathic infertile couples in Italy: Results from a multicentre, observational, clinical practice survey

et al. 2020

View full text Add to dashboard Cite

show abstract

“…43 A positive correlation between SDF and all markers of oxidative stress has been widely described in fertile and infertile men. 29,44,45 49,53 When the ORP was normalized to the motile sperm concentration, its power in predicting SDF markedly increased, basically reflecting the impact of seminal ROS on motility rather than inherent DNA breaks present in immature and aborted apoptotic spermatozoa, which are not phagocytosed and appear in the ejaculate. This is not surprising because sperm motility is the first parameter to be affected by oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Predictive value of oxidative stress testing in semen for sperm DNA fragmentation assessed by sperm chromatin dispersion test

et al. 2020

View full text Add to dashboard Cite

Background:Although standard semen analysis is a cornerstone of male infertility investiation, it has significant limitations. Seminal oxidative stress and DNA damage have shown potential to better predict male fertility potential and provide improved diagnostic and management strategies.Objective: The aim of this study was to investigate whether seminal oxidationreduction potential (ORP), a new parameter to measure oxidative stress directly, can accurately predict the percentage of sperm DNA fragmentation (SDF) and thereby serve as a surrogate marker in the evaluation of male infertility. Materials and methods: ORP was evaluated in 3968 patients using the MiOXSYS system and SDF in 1147 patients using the Halosperm G2 test kit. Both parameters were analyzed in 1068 patients, along with seminal analysis, according to WHO guidelines 2010 (5th edition).Results: SDF correlated positively with seminal ORP normalized for sperm concentration (n = 1068; r = .218; P < .0001) as well as with ORP normalized for the motile sperm concentration (motORP; n = 1068; r = .387; P < .0001). MotORP can significantly (P < .0001) better predict SDF than ORP normalized for the sperm concentration (area under the curve (AUC): 0.719 vs 0.623) (specificity: 71.5%; sensitivity: 61.9%; PPV: 47.1%; NPV: 82.1%). Moreover, motORP can significantly (P < .0001) better predict (AUC: 0.826 vs 0.771) normozoospermia with high sensitivity (82.75%) and specificity (68.5%).Discussion: Unlike other oxidative stress (OS) markers, ORP provides a global vision of the redox balance in semen. Moreover, SDF can also be induced by mechanisms different from OS, which could explain why its predictive power was low. The higher predictive power of motORP reflects the impact of seminal ROS on motility rather than inherent DNA breaks present in immature and aborted apoptotic spermatozoa. Conclusion:The evaluation of motORP seems a more promising parameter than ORP normalized for sperm concentration for the prediction of SDF and normozoospermia.However, even if ORP and SDF are inter-related, they measure independent sperm functions and one test cannot replace the other in the evaluation of sperm function defects. | 611ELBARDISI Et AL.

show abstract

“…SDF is primarily induced by defective maturation and abortive apoptosis occurring within the testis, or by OS throughout the male reproductive tract [17]. During spermatogenesis, chromatin is compacted through histone exchange with transitional proteins and protamines [18].…”

Section: Origin Of Sperm Dna Fragmentation 1 Primary Mechanisms Undementioning

confidence: 99%

Sperm DNA Fragmentation: A New Guideline for Clinicians

Agarwal

Majzoub

Baskaran

et al. 2020

World J Mens Health

175

144

View full text Add to dashboard Cite

Sperm DNA integrity is crucial for fertilization and development of healthy offspring. The spermatozoon undergoes extensive molecular remodeling of its nucleus during later phases of spermatogenesis, which imparts compaction and protects the genetic content. Testicular (defective maturation and abortive apoptosis) and post-testicular (oxidative stress) mechanisms are implicated in the etiology of sperm DNA fragmentation (SDF), which affects both natural and assisted reproduction. Several clinical and environmental factors are known to negatively impact sperm DNA integrity. An increasing number of reports emphasizes the direct relationship between sperm DNA damage and male infertility. Currently, several assays are available to assess sperm DNA damage, however, routine assessment of SDF in clinical practice is not recommended by professional organizations. This article provides an overview of SDF types, origin and comparative analysis of various SDF assays while primarily focusing on the clinical indications of SDF testing. Importantly, we report four clinical cases where SDF testing had played a significant role in improving fertility outcome. In light of these clinical case reports and recent scientific evidence, this review provides expert recommendations on SDF testing and examines the advantages and drawbacks of the clinical utility of SDF testing using Strength-Weaknesses-Opportunities-Threats (SWOT) analysis.

show abstract

Sperm DNA Fragmentation: Mechanisms of Origin

Cited by 62 publications

References 83 publications

Use of follicle‐stimulating hormone for the male partner of idiopathic infertile couples in Italy: Results from a multicentre, observational, clinical practice survey

Use of follicle‐stimulating hormone for the male partner of idiopathic infertile couples in Italy: Results from a multicentre, observational, clinical practice survey

Predictive value of oxidative stress testing in semen for sperm DNA fragmentation assessed by sperm chromatin dispersion test

Sperm DNA Fragmentation: A New Guideline for Clinicians

Contact Info

Product

Resources

About