Sperm DNA fragmentation is a novel biomarker for early pregnancy loss

Haddock, Lesley; Gordon, S.; Lewis, Sheena; Larsen, Peter B.; Shehata, Amjad; Shehata, Hassan

doi:10.1016/j.rbmo.2020.09.016

Cited by 38 publications

(20 citation statements)

References 38 publications

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“…In a prospective study, high Comet scores were associated with an eight‐fold increase in the risk of infertility (Simon et al, 2011). Furthermore, males with high DFI and Comet scores have reduced fertilization rates, embryo quality and pregnancy outcomes from IVF (Haddock et al, 2021; Simon et al, 2011; Virro et al, 2004) and ICSI (Simon et al, 2010; Virro et al, 2004). SCSA and Comet are also valuable in predicting miscarriage (Evenson et al, 1999; Yifu et al, 2020) and live birth rates (Osman et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

The relationship between genitourinary microorganisms and oxidative stress, sperm DNA fragmentation and semen parameters in infertile men

Vaughan-Constable

Ramsay

et al. 2021

Andrologia

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An imbalance in the genitourinary microbiome is emerging as a contributing factor to male infertility. The purpose of this study was to determine whether there is an association between genitourinary microorganisms and seminal oxidative stress, sperm DNA fragmentation and semen parameters. It included 770 men attending for diagnostic testing for subfertility. Genitourinary microorganisms were identified in 43.0% men; 20.1% had microorganisms in semen; 18.7% in urine; and 5.8% had microorganisms in urine and semen. Enterococcus faecalis was the most prevalent organism in semen (22.0% samples; 61.5% organisms) with Ureaplasma spp. (16.9% samples; 53.3% organisms) and Gardnerella vaginalis (11.4% samples; 37.4% organisms) most prevalent in urine. Semen parameters were unaffected by microorganisms (p > 0.05). Seminal ROS were significantly higher in men with microorganisms compared to those without (p < 0.001), particularly when present in both urine and semen (p < 0.01). Microorganisms were associated with significantly higher DNA fragmentation, irrespective of whether they were in semen or urine (p < 0.001). An imbalance in the genitourinary microbiome is associated with DNA damage and oxidative stress which may have considerable consequences for achieving an ongoing pregnancy. This highlights the need for incorporating genitourinary microorganism screening for all men as part of diagnostic evaluation prior to undergoing treatment for infertility.

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Section: Discussionmentioning

confidence: 99%

The relationship between genitourinary microorganisms and oxidative stress, sperm DNA fragmentation and semen parameters in infertile men

Vaughan-Constable

Ramsay

et al. 2021

Andrologia

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“…For the alkaline Comet assay (Donnelly et al 1999a,b;Haddock et al 2021), aliquots of native semen were adjusted using PBS to give a sperm concentration of 2 Â 10 6 mL À1 a n de m b e d d e di na g a r o s e . Embedded cells were then subjected to membrane lysis, protamine and histone removal, electrophoresis, SYBR Gold staining and Comet scoring (Komet 7.0, Andor Technologies, Belfast, UK) with analysis of 50 sperm cells per slide, in duplicate.…”

Section: Alkaline Cometmentioning

confidence: 99%

“…The relationship between DNAq and treatment outcome in ICSI is less clear, although miscarriage risk is elevated among couples where male partners have sperm with abnormally low DNAq (Robinson et al,2 0 1 2 ; Osman et al,2 0 1 5 ; Bach and Schlegel, 2016). These studies also suggest that there is an increased risk of miscarriage associated with the use of sperm from raw, unprocessed semen containing mixed cell populations compared with processed samples that are substantially cleared of poorer quality sperm, and Haddock et al (2021) reported similar sperm DNAq values associated with miscarriage following either natural conception or assisted conception (Robinson et al,2 0 1 2 ; Zhao et al,2 0 1 4 ; Coughlan et al, 2015;Cissen et al, 2016;Haddock et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Sperm Selection With Hyaluronic Acid Improved Live Birth Outcomes Among Older Couples and Was Connected to Sperm DNA Quality, Potentially Affecting All Treatment Outcomes

West

Coomarasamy

Frew

et al. 2022

Obstetrical &Amp; Gynecological Survey

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(Abstracted from Hum Reprod 2022;37:1106–1125)With intracytoplasmic sperm injection (ICSI), many natural barriers that would normally prevent the entry of abnormal sperm are lost, increasing the importance of sperm DNA integrity for successful in vitro fertilization. There is currently no consensus on the optimal assay for measuring sperm DNA quality (DNAq), defined as any structural aspect of sperm chromatin that can compromise function if disrupted.

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“…Additionally, diabetes has a detrimental impact on male and female reproductive function(s) [9,10]. Diabetic subjects often face disruption in sexuality where they lose their desire and become impotent and/or infertile [30], characterized by reduced sperm motility, elevated abnormal sperm morphology [9,31], higher sperm DNA damage, and deletion of mitochondrial DNA [32]. Besides, we hypothesized in the present study that diabetics exposed to cold stress may have to tackle sexual dysfunction and male infertility.…”

Section: Cumulative Impact Of Cold Stress On Sperm Quality Indices In Diabetesmentioning

confidence: 99%

Correlates of sperm quality parameters and oxidative stress indices in diabetic rats exposed to cold stress: Role of Moringa oleifera leaf extract

Mahaboob¹,

Hanumanthappa²

2021

J App Biol Biotech

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Spermatogenesis is extremely sensitive to fluctuations in the environment, particularly temperature and hormones. Sperm dysfunction, a root cause of male infertility, is a commonly allied complication of diabetes mellitus. Our previous studies cogitate that cold stress (15°C) exacerbates the complications and the resultant oxidative stress plays a major role in testicular and epididymis dysfunction in diabetic rats. Despite the strong biologic prospect for this postulation, establishing a direct link between free radicals and specific disease is an in-dire need, and in this context, this study focuses on investigating the sperm quality parameters and their relationship to testicular oxidative stress indices of cold stress diabetes in Wistar rats. The results indicate a cumulative impact by diminishing sperm parameters, viz. sperm density, viability, motility, mortality, and acrosome intactness in cold-stressed diabetic rats. The findings also reveal a strong positive Pearson's correlation between the sperm quality parameters and testicular lipid peroxidation, which reflects the influence of oxidative stress on sperm dysfunction. Together with duel stressor effects, the efficacy of Moringa oleifera leaf ethanolic (MOLE) extract is appended to assess its therapeutic role. The apparent effectiveness of MOLE therapy at 250 and 500 mg/kg bw for 60 days aided in suppressing oxidative stress and improved semen quality demonstrating the causative nature of these associations; hence, Moringa usage is recommended as a therapeutic agent for male reproductive dysfunctions in population residing in colder climates.

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Sperm DNA fragmentation is a novel biomarker for early pregnancy loss

Cited by 38 publications

References 38 publications

The relationship between genitourinary microorganisms and oxidative stress, sperm DNA fragmentation and semen parameters in infertile men

The relationship between genitourinary microorganisms and oxidative stress, sperm DNA fragmentation and semen parameters in infertile men

Sperm Selection With Hyaluronic Acid Improved Live Birth Outcomes Among Older Couples and Was Connected to Sperm DNA Quality, Potentially Affecting All Treatment Outcomes

Correlates of sperm quality parameters and oxidative stress indices in diabetic rats exposed to cold stress: Role of Moringa oleifera leaf extract

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