Sperm DNA fragmentation in boars is delayed or abolished by using sperm extenders

Llano, Begoña Pérez; Enciso, María; Garcı́a-Casado, Pedro; Sala, R.; Gosálvez, Jaime

doi:10.1016/j.theriogenology.2006.01.066

Cited by 43 publications

(29 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There were no significant differences in chromatin integrity among the samples in the first and second period in our studies. Similar findings were reported previously in boar semen by Perez-Llano et al (2006) [18], who found that sperm extenders may delay or partially prevent sperm DNA fragmentation. However, in our study, higher denaturation of DNA chromatin was observed in the third period in TG and CL samples compared with the TG+A TG and CL+A CL samples.…”

Section: Discussionsupporting

confidence: 92%

“…Results were analyzed using least square means (LSMs). Period 1 included days 1-8; period 2 included days 9-14; and period 3 included days [15][16][17][18][19][20][21][22][23]. Different superscript letters (a-c) indicate differences (P<0.05) among the extenders.…”

Section: Motilitymentioning

confidence: 99%

“…Components of the diluting media, centrifuging, and prolonged cold storage cause damage to the plasma membrane, acrosome, and DNA chromatin [15], [16] and [17]. Perez-Llano et al (2006) [18] found that sperm extenders delay, or partially prevent, sperm DNA fragmentation in boar spermatozoa. A strong correlation between the sperm chromatin structure assay (SCSA) and sperm head morphometry in dog spermatozoa has been found [19], but so far, only one study has been published on DNA fragmentation of dog semen [20].…”

Section: Introductionmentioning

confidence: 99%

“…The third replicate was examined for presence of bacteria. For the statistical calculations, the 23 experimental days were divided into three periods, the first period comprising days 1-8, the second period days 9-14, and the third period days [15][16][17][18][19][20][21][22][23]. Total motility (TM%) did not differ between extenders in the first period, but was higher in TG and TG+A TG compared with CL+A CL in the second and third periods, and with CL in the third period (P<0.05).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Induced immotility during long-term storage at +5°C does not prolong survival of dog spermatozoa

Shahiduzzaman

Linde‐Forsberg

2007

Theriogenology

View full text Add to dashboard Cite

The days are not so far away when sex sorted preserved liquid semen will be available in drug stores saying "Let me stay cool, I will give you beautiful puppies".To my parents (Abdus Satter and Jamela Khatun ) Preservation of canine semen by extension and chilling has provided the AI veterinarians with an opportunity to overcome limitations of both time and space. Several parameters are assessed to predict the fertility of canine spermatozoa for a given sample. Motility is one of them. Spermatozoa stay immotile while preserved in CLONE (Cryogenetic Laboratories of New England, Inc, USA) Chilled Semen Extender whereas they maintain motility over the whole 23 days period of study while preserved in a Tris egg yolk glucose extender at 5°C.The aim of this study was to investigate whether the immotility induced by the CLONE chilled semen extender prolongs the lifespan of dog spermatozoa stored at 5°C, compared with a Tris-egg yolk-glucose (TG) extender, which maintains motility. Semen from eleven dogs was pooled, split in four aliquots, centrifuged, and the supernatants removed. The four sperm pellets were mixed with a TG extender; with the CLONE chilled semen (CL) extender; with TG extender mixed with TG but without egg yolk and with less glucose (TG+A TG ); or with the CLONE extender mixed with the CLONE activator (CL+A CL ) to a final sperm concentration of 200 x 10 6 spermatozoa / mL. The samples were stored at 5ºC for 23 days and examined twelve times for sperm motility by computer-assisted sperm analysis (CASA), as well as for plasma membrane and acrosome integrity, glucose consumption, and DNA fragmentation index (DFI). The experiment was performed in triplicate. The third replicate was examined for presence of bacteria. For the statistical calculations, the 23 experimental days were divided into three periods, the first period comprising days 1-8, the second period days 9-14, and the third period days 15-23. Total motility (TM%) did not differ between extenders in the first period, but was higher in TG and TG+A TG compared with CL+A CL in the second and third periods, and with CL in the third period (P<0.05). Progressive motility (PM%) was higher in TG (P<0.0001), TG+A TG (P=0.0007) and CL+A CL (P=0.0176) than in CL in the first period. Both TG and TG+A TG maintained higher PM% than did CL and CL+A CL in the second and third period. Tris-egg yolkglucose and TG+A TG maintained higher straight line velocity (VSL) and average path velocity (VAP) compared with CL and CL+A CL during the whole experimental period. Curvilinear velocity (VCL) was higher in TG, CL, and TG+A TG than in CL+A CL samples (P<0.05) in the first and third periods. No differences in lateral head displacement (LHD) were observed between extenders or over time. Acrosome and plasma membrane integrity decreased over time, but there were no differences among the extenders in the first and second periods (P>0.05). In the third period, however, TG, TG+A TG , and CL maintained acrosome and plasma membrane integrity better than CL+A CL . Glucose consu...

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Motilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Induced immotility during long-term storage at +5°C does not prolong survival of dog spermatozoa

Shahiduzzaman

Linde‐Forsberg

2007

Theriogenology

View full text Add to dashboard Cite

show abstract

“…Some researchers have positively correlated the integrity of sperm chromatin with other qualitative semen variables such as motility and morphology (Foxcroft et al 2008). Others, such as Perez-Llano et al (2006) found that DNA fragmentation is negatively correlated with the evaluation of classic sperm variables. However, both studies agree that it is affected by semen handling and is associated with fertility and embryo development efficiency.…”

Section: P-valuementioning

confidence: 99%

A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility

et al. 2016

View full text Add to dashboard Cite

The present study compared the quality characteristics of boar semen diluted with three extenders of different proposed preservation times (short-term, medium-term and long-term). A part of extended semen was used for artificial insemination on the farm (30 sows/extender), while the remaining part was stored for three days (16-18 °C). Stored and used semen was also laboratory assessed at insemination time, on days 1 and 2 after the collection (day 0). The long-term extender was used for a short time, within 2 days from semen collection, with the aim to investigate a possible advantage over the others regarding laboratory or farm fertility indicators at the beginning of the preservation time. Viability, motility, kinetic indicators, morphology and DNA fragmentation were estimated. The results showed reduced viability, higher values for most of the kinetics, and higher immotile spermatozoa from day 1 to day 2 in all extenders; however, the long-term extender was superior compared to the other two on both days. With regard to morphology and chromatin integrity, the percentage of abnormal and fragmented spermatozoa increased on day 2 compared to day 1 for all of the extenders. However, based on the farrowing rate and the number of piglets born alive after the application of conventional artificial insemination within 2 days from semen collection/dilution, it was found that the medium-term diluents were more effective. In conclusion, it seems that the in vivo fertilization process involves more factors than simply the quality of laboratory evaluated sperm indicators, warranting further research. DNA fragmentation, ejaculate, in vitro quality, in vivo fertility, semen analysis, semen storageConventional examination of boar sperm on commercial farms aims to evaluate the fertilizing capacity and to control the conditions of breeding and sperm manipulation after the collection of the ejaculate (Tsakmakidis et al. 2010). Semen quality characteristics are affected by many factors, including the type of semen extender, storage duration and temperature (Fraser and Strzezek 2004).Boar semen diluents are classified by the commercial providers into three categories: short-term (ST), medium-term (MT) and long term (LT) extenders according to their preservation capacity (1-2, 3-4 and 7-10 days after collection, respectively). Long-term extenders allow for long distance sperm transportation. Moreover, the acquired time flexibility enables collection centres to conduct specialized diagnostic tests and to better organize semen procedures (Gadea 2003).In literature, only few studies compare the preservation capacity of different extenders in the laboratory and the relation with actual field conditions. To our knowledge, data on the efficacy of swine artificial insemination (AI) 1-2 days from collection and dilution in long-term extenders are even scarcer. This category of extenders is supposed to maintain sperm quality within acceptable levels (i.e. total sperm number > 35 × 10 9 sperm/ejaculate, gross motility > 70%, abnorma...

show abstract

Update on artificial insemination: Semen, techniques, and sow fertility

Mellagi

Will

Quirino

et al. 2022

Molecular Reproduction Devel

View full text Add to dashboard Cite

Over the years, reproductive efficiency in the swine industry has focused on reducing the sperm cell number required per sow. Recent advances have included the identification of subfertile boars, new studies in extended semen quality control, new catheters and cannulas for intrauterine artificial insemination (AI), and fixed‐time AI under commercial use. Therefore, it is essential to link field demands with scientific studies. In this review, we intend to discuss the current status of porcine AI, pointing out challenges and opportunities to improve reproductive efficiency.

show abstract

Sperm DNA fragmentation in boars is delayed or abolished by using sperm extenders

Cited by 43 publications

References 17 publications

Induced immotility during long-term storage at +5°C does not prolong survival of dog spermatozoa

Induced immotility during long-term storage at +5°C does not prolong survival of dog spermatozoa

A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility

Update on artificial insemination: Semen, techniques, and sow fertility

Contact Info

Product

Resources

About