Sperm DNA fragmentation in a random sample of the Spanish boar livestock

López‐Fernández, Carmen; Llano, Begoña Pérez; Garcı́a-Casado, Pedro; Sala, R.; Gosálbez, Altea; Arroyo, F.; Fernández, José Luis Fernández; Gosálvez, Jaime

doi:10.1016/j.anireprosci.2006.11.015

Cited by 32 publications

(19 citation statements)

References 33 publications

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“…Conversely, in other mammals, correlation was found in fertile but not subfertile stallions using a TUNEL assay for DNA damage determination, although in this case magnitude was not high (17). When sperm morphology is considered, a negative correlation is usually reported (18,19). The correlation most frequently reported for DNA fragmentation is a high negative relationship with sperm motility, either total or progressive.…”

Section: Figurementioning

confidence: 93%

A dynamic assessment of sperm DNA fragmentation versus sperm viability in proven fertile human donors

Gosálvez

Cortés‐Gutiérrez

Núñez³

et al. 2009

Fertility and Sterility

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Section: Figurementioning

confidence: 93%

A dynamic assessment of sperm DNA fragmentation versus sperm viability in proven fertile human donors

Gosálvez

Cortés‐Gutiérrez

Núñez³

et al. 2009

Fertility and Sterility

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“…In this sense Parrilla et al (2012) showed that ejaculate spermatozoa from individual boars can respond in a boar-dependent manner to different semen-processing techniques. It could be then hypothesized that different qualitative characteristics, such as DNA fragmentation index (Lopez-Fernandez et al 2008) of sperm samples, could be compromising the TX pre-treatment reproducibility within different boar samples and could be the basis of the different fertilization rates obtained from different males, since DNA fragmentation in boar sperm has been correlated with poor fertility outcomes (Boe-Hansen et al 2008;Didion et al 2009). Moreover, in further experiments it could be interesting to study the effect of the TX treatment in later embryo development stages.…”

Section: Discussionmentioning

confidence: 99%

Male pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatments

García-Mengual¹,

Silvestre²,

Salvador³

et al. 2015

CZECH J ANIM SCI

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ABSTRACT:In pigs, intracytoplasmic sperm injection (ICSI) efficiency is still poor. The inadequate decondensation of the sperm chromatin, its transformation into the male pronucleus (MPN) together with the subsequent inability to activate the oocyte, seem to be the main causes of the low ICSI efficiency. In order to improve the MPN formation we took two different approaches. On the one hand, the in vitro culture (IVC) medium post-ICSI was supplemented with 1.71mM cysteine (CYS). Alternatively, the sperm membrane was digested with Triton X-100 (TX) before ICSI, to improve the exposure of the sperm chromatin to the oocyte cytoplasm. After 6 h post-ICSI, the activation rate was significantly higher in TX group (70.0%) compared with CYS and control groups (42.2% and 48.9%, respectively; P < 0.05). However, no significant differences between the three groups were observed in terms of the number of pronuclei, 2PN (oocytes with 2 pronuclei and no visible sperm), and 1PN + sperm (oocytes with 1 pronucleus and one sperm head). At 22 h post-ICSI, the activation rates were similar in TX, CYS, and control groups (73.1, 78.9, and 75.7%, respectively). In addition, we did not observe significant differences between TX, CYS, and control groups for the number of pronuclei, 2PN (52.6, 56.7, and 50%, respectively) or 1PN + sperm (21.1, 33.3, and 32.1%, respectively). While no cleavage was observed in the CYS group, no significant differences in the cleavage rate were observed between control (21.3%) and TX (10.5%) groups. In summary, and under our conditions, neither CYS supplement, nor sperm TX pre-treatment were able to improve MPN formation at 6 and 22 h post-ICSI. However, the sperm TX pre-treatment improved oocyte activation at 6 h post-ICSI, although 22 h post-ICSI such a beneficial effect did not persist.Keywords: porcine; assisted reproductive technology; pronuclear formation List of abbreviations: ICSI = intracytoplasmic sperm injection, PN = pronucleus, MPN = male pronucleus, IVC = in vitro culture, CYS = cysteine, TX = Triton X-100, IVP = in vitro production, IVM = in vitro maturation, IVF = in vitro fertilization, EGF = epidermal growth factor, GSH = glutathione, COC = cumulus oocyte complex, PZM-3 = porcine zygote medium-3, MM199 = maturation medium, HM199 = handling medium 242

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“…The prevalence as well as the incidence of abnormal chromatin structure in spermatozoa from human and domestic animal species varies greatly. In the present and in other studies (López-Fernández et al 2008), the incidence of chromatin instability in boar sperm has been found to be generally low and has not shown a constant correlation with any one sperm defect except for attached cytoplasmic droplets. A correlation between the susceptibility of DNA to denaturation and sperm cytoplasmic droplets was also observed in human (Fischer et al 2003).…”

Section: Discussionmentioning

confidence: 74%

“…(Khalil et al 2006). Whereas cytoplasmic droplets present a quite common sperm abnormality in boar ejaculates, the incidence of chromatin instability seems to be relatively low in commercial boar semen (López-Fernández et al 2008). However, it has been suggested that even low numbers of sperm (5-10%) with clearly disturbed chromatin structure may imply the presence of undiagnosed chromatin disturbances in a larger population of sperm (Evenson et al 2002).…”

Section: Discussionmentioning

confidence: 97%

Chromatin-unstable boar spermatozoa have little chance of reaching oocytes in vivo

Ardón¹,

Helms²,

Sahin³

et al. 2008

Reproduction

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In the present study, the prevalence of chromatin instability in the fertilizing-competent sperm population in the porcine oviduct in vivo was examined through qualitative analysis of the chromatin structure status of accessory boar sperm found in in vivo-derived embryos. The binding of chromatin-unstable sperm to oviductal epithelium in vitro was also studied. To examine the sperm chromatin state, a modified fluorescence microscopic sperm chromatin structure assay was used. Among a population of 173 fertile boars, individuals were selected for according to their chromatin status: 25 animals showed more than 5% of chromatin-unstable sperm in their ejaculates, and 7 showed consistently elevated percentages of chromatin-unstable sperm in three successively collected semen samples. A positive correlation was found between incidence of chromatin instability and attached cytoplasmic droplets (rZ0.44, P!0.01). Analyses of accessory spermatozoa from in vivo-derived embryos demonstrated that the proportion of chromatin-unstable sperm was significantly (P!0.05) reduced in the population of fertilizing-competent sperm in the oviduct compared with the inseminated sperm. Populations of sperm bound to the oviduct in vitro had significantly (P!0.05) lower percentages of chromatin instability than in the original diluted semen sample. In conclusion, numbers of sperm with unstable chromatin are reduced in the oviductal sperm reservoir, possibly because of associated changes in the plasma membrane that prevent sperm from binding to the oviductal epithelium. We conclude that in vivo the likelihood that sperm with unstable chromatin will reach the egg and fertilize it is low.

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Sperm DNA fragmentation in a random sample of the Spanish boar livestock

Cited by 32 publications

References 33 publications

A dynamic assessment of sperm DNA fragmentation versus sperm viability in proven fertile human donors

A dynamic assessment of sperm DNA fragmentation versus sperm viability in proven fertile human donors

Male pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatments

Chromatin-unstable boar spermatozoa have little chance of reaching oocytes in vivo

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