Sperm DNA Fragmentation Assays Correlate with Sperm Abnormal Morphology and the Pregnancy Outcome

Fortunato, Adriana; Leo, Rita

doi:10.4172/jfiv.1000101

Cited by 3 publications

(4 citation statements)

References 20 publications

(21 reference statements)

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“…A total of 200 cells were evaluated on each slide Delbes et al, 2013;Fortunato et al, 2013). The sperm suspension was first centrifuged for 5 min at 300 g. The supernatant was discarded and the pellet was washed in phosphate-buffered saline (19 PBS), Sigma-Aldrich, SP, Brazil.…”

Section: Sperm Dna Fragmentation Detection (Tunel Methods)mentioning

confidence: 99%

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Magnetic‐activated cell sorting before density gradient centrifugation improves recovery of high‐quality spermatozoa

et al. 2017

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Recent studies have evaluated the use of magnetic-activated cell sorting (MACS) to reduce apoptotic spermatozoa and improve sperm quality. However, the efficiency of using MACS alone, before or after sperm processing by density gradient centrifugation (DGC) has not yet been established. The purpose of this study is to determine the optimal protocol of MACS in assisted reproduction techniques (ART). Thus, we compared sperm quality obtained by DGC alone (DGC), DGC followed by MACS (DGC-MACS), MACS followed by DGC (MACS-DGC), and MACS alone (MACS), and found that the combined methods (MACS-DGC and DGC-MACS) led to retrieval of less spermatozoa with fragmented DNA compared to the single protocols. However, MACS-DGC protocol led to a significantly higher percentage of spermatozoa with progressive motility and normal morphology than DGC-MACS protocol. These findings suggest the potential clinical value of using MACS-DGC to improve sperm quality in seminal preparation for ART.

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Section: Sperm Dna Fragmentation Detection (Tunel Methods)mentioning

confidence: 99%

“…Samples were analyzed using a fluorescence inverted microscope Nikon Eclipse E200 (Nikon Corp., Tokyo, Japan). A total of 200 cells were evaluated on each slide Delbes et al, 2013;Fortunato et al, 2013). Cells with DNA damage were considered TUNEL positive (apoptotic) (Fig.…”

Section: Sperm Dna Fragmentation Detection (Tunel Methods)mentioning

confidence: 99%

Magnetic‐activated cell sorting before density gradient centrifugation improves recovery of high‐quality spermatozoa

et al. 2017

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“…Therefore, abnormal sperm chromatin may result in male infertility, recurrent abortion, increased congenital anomalies , testicular cancer in offsprings as well as assisted reproductive failure (6 , 7) . It was found that the compact structure of sperm nuclear chromatin is important for the protection of genetic integrity during transport of the paternal genome through the male and female reproductive tracts (8) .…”

Section: Introductionmentioning

confidence: 99%

Using Sperm chromatin Staining Techniques as a Predictive Diagnostic Tool for Male Infertility

Al-Fahham

Al-Sultani

Muhammad-Ali

2014

Kufa Jour. Nurs. Sci.

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Background: It is a scientific fact today that routine seminal analysis is only a poor predictor of male reproductive potentiality, so that there is an increased need for finding out new functional tests in the assessment of male infertility. Objective: The aim of the present study was to investigate the predictive value of sperm chromatin integrity assays as a complementary tool to assist in diagnosis of infertility. Methodology: This study was carried out between January 2013 and November 2013 including one hundred (100)selected infertile men who attended to fertility Center in Al-Sadr Medical City. The study also included twenty (20) healthy fertile volunteers as a control. Aniline blue (AB) staining was used to differentiate between normal condensed chromatin (unstained) and abnormal decondensed chromatin (blue), while toluidine blue (TB) was use to differentiate between normal intact sperm DNA (blue) and abnormal damaged sperm DNA (purple), this is the first study in Iraq that employed (TB) to assess sperm DNA fragmentation. Results: The results showed that there was a high significant difference (p<0.01) in sperm chromatin decondensation and sperm DNA damage between infertile and fertile men. The study also revealed a significantdifference (p<0.05) in sperm DNA damage between normozoospermic and fertile men, but no significant difference (p>0.05) was found in the sperm chromatin condensation. Both sperm chromatin condensation and sperm DNA damage showed a significant positive correlation with sperm morphology, and a significant negative correlation with sperm concentration, motility and progressive motility. Conclusions: The study concluded that sperm DNA fragmentation (by TB staining) has good predictive value in discriminating infertile and fertile men over seminal fluid analysis. Recommendations: TB and AB staining of sperm chromatin can be used in the course of infertility diagnosis. However, more investigations may be needed to confirm the discriminating power of sperm chromatin condensation assays (by AB staining) to be ready to use in the routine workup in the fertility clinics.

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“…The patient characteristics are shown in Table 1. Ovulation induction and embryology protocols and procedures have been published previously (Fishel et al, 2011). In the tested and treated group, if either or both of the couple were carriers of the M2 haplotype, the female received LMWH (enoxaparin: Clexane; Sanofi Winthrop, France) subcutaneously in a dose of 40 mg daily beginning on the day of oocyte retrieval for fresh embryo transfer or, on the day of frozen embryo transfer, and for a minimum of 12 weeks, with the recommendation to continue until term.…”

Section: Methodsmentioning

confidence: 99%

Precision Medicine in Assisted Conception: A Multicenter Observational Treatment Cohort Study of the Annexin A5 M2 Haplotype as a Biomarker for Antithrombotic Treatment to Improve Pregnancy Outcome

Fishel

Baker

Elson

et al. 2018

Obstetrical &Amp; Gynecological Survey

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Background: Pregnancy failure and placenta mediated pregnancy complications affect N 25% of pregnancies. Although there is biological plausibility for a procoagulant mechanism underlying some of these events, antithrombotic intervention trials demonstrate limited benefit, possibly through lack of stratification in heterogeneous patient groups. The ANXA5 M2 haplotype is a possible procoagulant biomarker and was tested pragmatically to determine whether this screening and LMWH treatment normalized the outcome for ANXA5 M2 positive couples. This was a pragmatic study that aimed to measure the effectiveness of a testing (for the M2 haplotype) and treatment (LMWH) pathway in routine clinical practice where there is variation between patients. Such a study in couples with fertility problems can inform choices between treatments; it is then the management protocol which is the subject of the investigation, not the individual treatments. Methods: Couples (N = 77) with one or both partners ANXA5 M2 positive demonstrated association of this haplotype with adverse IVF outcome. A pragmatic, multicenter, prospective cohort study of ANXA5 M2 haplotype screening, and LWMH treatment following embryo transfer (ET) in 103 IVF couples positive for ANXA5 M2 was performed. They were compared with a group of 1000 contemporaneous randomly selected unscreened and untreated couples undergoing assisted conception, from which 103 matched control couples were derived. The primary outcome measure was live birth incidence. Secondary outcomes were results following embryo transfer (ET) and live birth outcome by gender and M2 carriage, and allelic dose influence. Findings: The tested and treated cohort of ANXA5 M2 carriers achieved a similar live birth rate (37.9%) per ET cycle compared to both the more fertile comparison group (38.5%), and to the 103 matched controls (33.0%). Significantly more treated male carrier only couples had a live birth versus female M2 only (47.7% vs. 25.0% p = 0.045). Interpretation: Pragmatic ANXA5 M5 screening and treatment with LMWH in couples undergoing IVF is associated with similar outcome to couples with more favorable prognostic factors. The difference in live birth outcome for treated male only carrier couples may be consistent with an additional maternal thrombophilic factor that may adversely affect pregnancy, although other mechanisms are possible. This study suggests that LMWH treatment should be started prior to clinical pregnancy.

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Sperm DNA Fragmentation Assays Correlate with Sperm Abnormal Morphology and the Pregnancy Outcome

Abstract: JFIV, an open access journal J o u rn al of Fe rt il iz a tio n: In V it ro-I V F-Worldw id e

Cited by 3 publications

References 20 publications

Magnetic‐activated cell sorting before density gradient centrifugation improves recovery of high‐quality spermatozoa

Magnetic‐activated cell sorting before density gradient centrifugation improves recovery of high‐quality spermatozoa

Using Sperm chromatin Staining Techniques as a Predictive Diagnostic Tool for Male Infertility

Precision Medicine in Assisted Conception: A Multicenter Observational Treatment Cohort Study of the Annexin A5 M2 Haplotype as a Biomarker for Antithrombotic Treatment to Improve Pregnancy Outcome

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