Sperm DNA fragmentation affects epigenetic feature in human male pronucleus

Rajabi, Hoda; Mohseni-Kouchesfehani, Homa; Eslami-Arshaghi, Tarlan; Salehi, Mohammad

doi:10.1111/and.12800

Cited by 9 publications

(7 citation statements)

References 39 publications

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“…When DNA fragmentation is more than 30%, there is an inverse relationship between DFI and level of H4K12ac in male pronuclei. These findings demonstrate that increased level of damage results in a decreased level of sperm H4K12 acetylation, because DNA strand breaks are likely to cause inappropriate localization of acetylated histones in male chromatin [192].…”

Section: Histone Ptms In Spz: Their Implication In Sperm Quality and mentioning

confidence: 74%

Histone Post-Translational Modifications and CircRNAs in Mouse and Human Spermatozoa: Potential Epigenetic Marks to Assess Human Sperm Quality

Chioccarelli

Pierantoni

Manfrevola

et al. 2020

JCM

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Spermatozoa (SPZ) are motile cells, characterized by a cargo of epigenetic information including histone post-translational modifications (histone PTMs) and non-coding RNAs. Specific histone PTMs are present in developing germ cells, with a key role in spermatogenic events such as self-renewal and commitment of spermatogonia (SPG), meiotic recombination, nuclear condensation in spermatids (SPT). Nuclear condensation is related to chromatin remodeling events and requires a massive histone-to-protamine exchange. After this event a small percentage of chromatin is condensed by histones and SPZ contain nucleoprotamines and a small fraction of nucleohistone chromatin carrying a landascape of histone PTMs. Circular RNAs (circRNAs), a new class of non-coding RNAs, characterized by a nonlinear back-spliced junction, able to play as microRNA (miRNA) sponges, protein scaffolds and translation templates, have been recently characterized in both human and mouse SPZ. Since their abundance in eukaryote tissues, it is challenging to deepen their biological function, especially in the field of reproduction. Here we review the critical role of histone PTMs in male germ cells and the profile of circRNAs in mouse and human SPZ. Furthermore, we discuss their suggested role as novel epigenetic biomarkers to assess sperm quality and improve artificial insemination procedure.

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Section: Histone Ptms In Spz: Their Implication In Sperm Quality and mentioning

confidence: 74%

Histone Post-Translational Modifications and CircRNAs in Mouse and Human Spermatozoa: Potential Epigenetic Marks to Assess Human Sperm Quality

Chioccarelli

Pierantoni

Manfrevola

et al. 2020

JCM

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“…Prepronuclei is a reform stage of the nuclear envelope. Pronuclei is the last stage of sperm transformation with conspicuous of nuclear envelope (Lassalle and Testart, 1991;Rajabi et al, 2017). Evaluation was conducted using phase contrast microscope (Olympus XI, Japan).…”

Section: In Vitro Fertilization and Evaluation Of Transformation Spermentioning

confidence: 99%

“…Prepronuclei is a reform stage of the nuclear envelope. When the nuclear envelope seen clearly, the transformation known as pronuclei (Rajabi et al, 2017). Pronuclei is the last stage of sperm transformation with conspicuous of nuclear envelope, completion of nucleolus precursor development, advance change on chromatin distribution, and nuclear envelope modification (Lassalle and Testart, 1991).…”

Section: Introductionmentioning

confidence: 99%

Transformation of ram sperm nuclei in oocytes cytoplasm during in vitro fertilization

Dzulfiqor

Setiadi

Karja

2019

J. Indonesian Trop. Anim. Agric.

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ABSTRAKTujuan penelitian ini adalah untuk memahami transformasi inti sperma domba di dalam sitoplasma oosit saat fertilisasi secara in vitro. Oosit didapatkan dari rumah potong hewan. Sebelum fertilisasi, oosit dimatangkan secara in vitro selama 24 jam. Oosit (n= 635) kemudian difertilisasi dengan sperma (5x10 6 spermatozoa/ ml) dalam inkubator dengan 5% CO 2 pada 38.5°C. Proses inkubasi dilakukan selama 3, 6, 9, 12, dan 15 jam untuk setiap perlakuan. Setelah fertilisasi selesai, oosit difiksasi dan diwarnai dengan aceto-orcein 2% sebelum dievaluasi dengan mikroskop fase kontras. Transformasi inti sperma dievaluasi sesuai dengan status inti dari sperma, seperti kondensasi, dekondensasi, dan pembentukan prepronukleus dan pronukleus. Kondensasi dan dekondensasi sperma terlihat pada tiga jam setelah inkubasi sperma dengan oosit. Enam jam setelah inkubasi ditemukan prepronukelus dan pronukelus. Pronukleus mulai terbentuk pada enam jam setelah inkubasi dan secara nyata meningkat pada sembilan jam setelah inkubasi (P<0.05). Kejadian polispermia mengalami peningkatan secara signifikan pada 12-15 jam setelah interaksi sperma dengan oosit (P<0.05). Disimpulkan bahwa penetrasi sperma ke dalam sitoplasma oosit sudah terjadi pada tiga jam periode fertilisasi. Pronukleus sudah terbentuk pada 6 jam setelah inkubasi dan kejadian polispermia meningkat seiring dengan bertambahnya waktu fertilisasi.Kata kunci: domba, fertilisasi in vitro, inti spermatozoa, transformasi . ABSTRACTThe aim of present study was to understand the transformation of ram sperm nuclei within oocyte cytoplasm during in vitro fertilization. The oocytes were collected from slaughterhouse ovaries. Before fertilization, the oocytes were maturated in vitro for 24 hours in the incubator with 5% CO 2 at 38.5°C. Then the oocytes (n= 635) was fertilized by incubating the oocytes with sperm (5x10 6 spermatozoa/ ml) for 3, 6, 9, 12, and 15 hours. At the end of incubating period, the oocytes were fixed and stained with aceto-orcein 2% before evaluated under phase contrast microscope. Sperm nuclear transformation was evaluated according to sperm nuclear status of sperm, such as condensation, decondensation, and formation of prepronuclei and pronuclei. Sperm condensation and decondensation were seen at 3 hours after incubation. Prepronuclei and pronuclei were found at 6 hours of incubation. Pronuclei formation was significantly increased in the 9 hours after incubation (P<0.05). The incidence of polyspermia was significantly increased at 12-15 hours after incubation (P<0.05). In conclusion penetration of sperm into oocytes has been occurred at 3 hour of fertilization period. The formation of pronuclei was found at 6 hours after incubation and the incidence of polyspermia was increased when the fertilization period 146

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“…An elevated level of sperm DNA damage is a clinical issue in human reproductive medicine due to its associations with reduced fertilization rates [ 1 , 2 , 3 ], poor embryo development [ 2 , 4 , 5 , 6 , 7 , 8 , 9 ] and pregnancy loss [ 10 , 11 , 12 , 13 , 14 , 15 ]. Because sperm DNA damage may act as a significant barrier to successful conception, an effective treatment is wanting.…”

Section: Introductionmentioning

confidence: 99%

Mitigating the Effects of Oxidative Sperm DNA Damage

et al. 2020

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Sperm DNA damage is correlated with reduced embryo development and increased miscarriage risk, reducing successful conception. Given its links with oxidative stress, antioxidants have been investigated as a potential treatment, yet results are conflicting. Importantly, individual antioxidants are not identical in composition, and some compounds may be more effective than others. We investigated the use of the polyphenol-rich, high-antioxidant-capacity fruit acai as a treatment for elevated sperm DNA fragmentation (>16%), measured by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). Following ≥ 74 days of treatment, we observed a significant decrease in sperm DNA fragmentation (−17.0% ± 2.5%) to 11.9 ± 1.7% (0–37%), with a 68.6% success rate (defined as post-treatment TUNEL < 16%). Post-treatment decreases in DNA fragmentation and success rates were not significantly impacted by low motility and/or concentration, or exceptionally high (> 25%) TUNEL. Treatment significantly reduced concentration in men with normal semen parameters, but 88% remained normal. Overall, successful treatment was not associated with age, semen parameters or TUNEL result at baseline. However, body mass index was significantly higher in nonresponders at baseline. This study provides evidence of a low-cost, effective treatment for elevated sperm DNA damage using acai.

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Sperm DNA fragmentation affects epigenetic feature in human male pronucleus

Cited by 9 publications

References 39 publications

Histone Post-Translational Modifications and CircRNAs in Mouse and Human Spermatozoa: Potential Epigenetic Marks to Assess Human Sperm Quality

Histone Post-Translational Modifications and CircRNAs in Mouse and Human Spermatozoa: Potential Epigenetic Marks to Assess Human Sperm Quality

Transformation of ram sperm nuclei in oocytes cytoplasm during in vitro fertilization

Mitigating the Effects of Oxidative Sperm DNA Damage

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