Sperm DNA damage in potentially fertile homozygous  -thalassaemia patients with iron overload

Perera, Doreen M.D.; Pizzey, Arnold; Campbell, Alastair; Katz, Maurice; Porter, John P.; Petrou, Mary; Irvine, D. Stewart; Chatterjee, Ratna

doi:10.1093/humrep/17.7.1820

Cited by 63 publications

(35 citation statements)

References 29 publications

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“…Consistent results were also reported for the effect of sodium nitroprusside on sperm kinematic parameters assessed in this study (Table 1). Clinically, higher levels of NO have been reported in infertile men with decreased sperm motility, whereas inhibition of NO synthase improved sperm motility (Nobunaga et al, 1996;Perera et al, 2002;Wu et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Sodium nitroprusside suppresses male fertility in vitro

et al. 2014

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SUMMARYSodium nitroprusside is a nitric oxide donor involved in the regulation of the motility, hyperactivation, capacitation, and acrosome reaction (AR) of spermatozoa. However, the molecular mechanism underlying this regulation has not yet been elucidated. Therefore, this study was designed to evaluate the molecular basis for the effects of sodium nitroprusside on different processes in spermatozoa and its consequences on subsequent oocyte fertilization and embryo development. In this in vitro study, mouse spermatozoa were incubated with various concentrations of sodium nitroprusside (1, 10, and 100 lM) for 90 min. Our results showed that sodium nitroprusside inhibited sperm motility and motion kinematics in a dose-dependent manner by significantly enhancing intracellular iron and reactive oxygen species (ROS), and decreasing Ca 2+ , and adenosine triphosphate levels in spermatozoa. Moreover, short-term exposure of spermatozoa to sodium nitroprusside increased the tyrosine phosphorylation of sperm proteins involved in PKA-dependent regulation of intracellular calcium levels, which induced a robust AR. Finally, sodium nitroprusside significantly decreased the rates of fertilization and blastocyst formation during embryo development. Based on these results, we propose that sodium nitroprusside increases ROS production and precocious AR may alter overall sperm physiology, leading to poor fertilization and compromised embryonic development.

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Section: Discussionmentioning

confidence: 99%

Sodium nitroprusside suppresses male fertility in vitro

et al. 2014

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“…In relation to Cu, this dependence has additionally complex arrangement and frequently synergetic character in connection with their cooperation in redox processes. So, although Fe and its compounds are not toxic for human and animals, its overload can cause an increase in sperm DNA damage [19]. However, significant differences in Fe concentration between sperm of several teratospermia and normozoospermia subjects were not found [20].…”

Section: Introductionmentioning

confidence: 98%

Zinc and Iron Concentration and SOD Activity in Human Semen and Seminal Plasma

Marzec-Wróblewska

Kamiński

Łakota

et al. 2010

Biol Trace Elem Res

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The aim of the present study was to measure zinc (Zn) and iron (Fe) concentration in human semen and superoxide dismutase (SOD) activity in seminal plasma and correlate the results with sperm quality. Semen samples were obtained from men (N = 168) undergoing routine infertility evaluation. The study design included two groups based on the ejaculate parameters. Group I (n = 39) consisted of males with normal ejaculate (normozoospermia), and group II (n = 129) consisted of males with pathological spermiogram. Seminal Zn and Fe were measured in 162 samples (group I, n = 38; group II, n = 124) and SOD activity in 149 samples (group I, n = 37; group II, n = 112). Correlations were found between SOD activity and Fe and Zn concentration, and between Fe and Zn concentration. SOD activity was negatively associated with volume of semen and positively associated with rapid progressive motility, nonprogressive motility, and concentration. Negative correlation was stated between Fe concentration and normal morphology. Mean SOD activity in seminal plasma of semen from men of group I was higher than in seminal plasma of semen from men of group II. Fe concentration was higher in teratozoospermic males than in males with normal morphology of spermatozoa in group II. Our results suggest that Fe may influence spermatozoa morphology.

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“…Treating infertile male patients with β-thalassemia with growth hormone and gonadotropins improved semen parameters [105]. Furthermore, cryopreservation is recommended to thalassaemia patients prior to a full manifestation of the toxic effects of the disease [106].…”

Section: Male Reproductive Complications Associated With Iron and Copmentioning

confidence: 99%

“…Moreover, a collection of studies have concluded that Fe deposition and accumulation can be detrimental to sperm DNA. Oxidative damage to sperm DNA generated by iron were dose dependent, leading to apoptosis and a shorter sperm life span [106,135].…”

Section: Coppermentioning

confidence: 99%

Iron and copper in male reproduction: a double-edged sword

Tvrdá

Peer

Sikka

et al. 2014

J Assist Reprod Genet

153

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Iron and copper are essential trace nutrients playing important roles in general health and fertility. However, both elements are highly toxic when accumulating in large quantities. Their direct or indirect impact on the structure and function of male gonads and gametes is not completely understood yet. Excess or deficiency of either element may lead to defective spermatogenesis, reduced libido, and oxidative damage to the testicular tissue and spermatozoa, ultimately leading to fertility impairment. This review will detail the complex information currently available on the dual roles iron and copper play in male reproduction.

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Sperm DNA damage in potentially fertile homozygous -thalassaemia patients with iron overload

Cited by 63 publications

References 29 publications

Sodium nitroprusside suppresses male fertility in vitro

Sodium nitroprusside suppresses male fertility in vitro

Zinc and Iron Concentration and SOD Activity in Human Semen and Seminal Plasma

Iron and copper in male reproduction: a double-edged sword

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