Sperm Differentiation: The Role of Trafficking of Proteins

Teves, María E.; Roldán, Eduardo R. S.; Krapf, Diego; Strauss, Jerome F.; Bhagat, Virali; Sapao, Paulene

doi:10.3390/ijms21103702

Cited by 35 publications

(49 citation statements)

References 239 publications

(226 reference statements)

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“…Interestingly, in mice, the IFT20 was identified as a Spef2 partner protein in maturing sperm cells 48 . In mammals, the differentiation of spermatids requires an intense transport of cargoes along the manchette microtubules and this process involves intraflagellar transport proteins 49 . However, how Spef2 is related to intraflagellar and IFT-related inter-manchette transport is to be determined.…”

Section: Discussionmentioning

confidence: 99%

Composition and function of the C1b/C1f region in the ciliary central apparatus

Joachimiak

Osinka

Farahat

et al. 2021

Sci Rep

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Motile cilia are ultrastructurally complex cell organelles with the ability to actively move. The highly conserved central apparatus of motile 9 × 2 + 2 cilia is composed of two microtubules and several large microtubule-bound projections, including the C1b/C1f supercomplex. The composition and function of C1b/C1f subunits has only recently started to emerge. We show that in the model ciliate Tetrahymena thermophila, C1b/C1f contains several evolutionarily conserved proteins: Spef2A, Cfap69, Cfap246/LRGUK, Adgb/androglobin, and a ciliate-specific protein Tt170/TTHERM_00205170. Deletion of genes encoding either Spef2A or Cfap69 led to a loss of the entire C1b projection and resulted in an abnormal vortex motion of cilia. Loss of either Cfap246 or Adgb caused only minor alterations in ciliary motility. Comparative analyses of wild-type and C1b-deficient mutant ciliomes revealed that the levels of subunits forming the adjacent C2b projection but not C1d projection are greatly reduced, indicating that C1b stabilizes C2b. Moreover, the levels of several IFT and BBS proteins, HSP70, and enzymes that catalyze the final steps of the glycolytic pathway: enolase ENO1 and pyruvate kinase PYK1, are also reduced in the C1b-less mutants.

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Section: Discussionmentioning

confidence: 99%

Composition and function of the C1b/C1f region in the ciliary central apparatus

Joachimiak

Osinka

Farahat

et al. 2021

Sci Rep

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“… – [ 118 ] LINC00326 C9orf152, FLJ35934, GNB5 , LINC00940, GNB5 could play a role in acrosome biogenesis and vesicle trafficking. – [ 119 ] LINC00494 ABI2, EMC10, FAM98B, GOLGA6L22, GOLGA6L6 , IGFN1, RP11-573D15.8, RPGR , SNX8 – – – LINC00535 ABI2, FAM98B , SNX8 – – – LINC00616 ABI2, CCDC85C, EMC10, FAM98B , MLYCD, PARVG, RP11-573D15.8, RP3-323A16.1, RPGR , SNX8 – – – LINC00662 RP11-573D15.8 – LIN28A- involved in cell metastasis and stemness in lung cancer. – LINC00668 ABI2, EMC10, KCNQ1OT1 , MIR6820, RP11-573D15.8, RP3-323A16.1, RPGR , SNX8 KCNQ1OT1 is paternally expressed and plays a role in silencing of six genes Ascl2, Kcnq1, Cdkn1c, Slc22a18, Phlda2, and Osbpl5.…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNAs (lncRNAs) in spermatogenesis and male infertility

Joshi

Rajender

2020

Reprod Biol Endocrinol

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Background Long non-coding RNAs (lncRNAs) have a size of more than 200 bp and are known to regulate a host of crucial cellular processes like proliferation, differentiation and apoptosis by regulating gene expression. While small noncoding RNAs (ncRNAs) such as miRNAs, siRNAs, Piwi-interacting RNAs have been extensively studied in male germ cell development, the role of lncRNAs in spermatogenesis remains largely unknown. Objective In this article, we have reviewed the biology and role of lncRNAs in spermatogenesis along with the tools available for data analysis. Results and conclusions Till date, three microarray and four RNA-seq studies have been undertaken to identify lncRNAs in mouse testes or germ cells. These studies were done on pre-natal, post-natal, adult testis, and different germ cells to identify lncRNAs regulating spermatogenesis. In case of humans, five RNA-seq studies on different germ cell populations, including two on sperm, were undertaken. We compared three studies on human germ cells to identify common lncRNAs and found 15 lncRNAs (LINC00635, LINC00521, LINC00174, LINC00654, LINC00710, LINC00226, LINC00326, LINC00494, LINC00535, LINC00616, LINC00662, LINC00668, LINC00467, LINC00608, and LINC00658) to show consistent differential expression across these studies. Some of the targets of these lncRNAs included CENPB, FAM98B, GOLGA6 family, RPGR, TPM2, GNB5, KCNQ10T1, TAZ, LIN28A, CDKN2B, CDKN2A, CDKN1A, CDKN1B, CDKN1C, EZH2, SUZ12, VEGFA genes. A lone study on human male infertility identified 9879 differentially expressed lncRNAs with three (lnc32058, lnc09522, and lnc98497) of them showing specific and high expression in immotile sperm in comparison to normal motile sperm. A few lncRNAs (Mrhl, Drm, Spga-lncRNAs, NLC1-C, HongrES2, Tsx, LncRNA-tcam1, Tug1, Tesra, AK015322, Gm2044, and LncRNA033862) have been functionally validated for their roles in spermatogenesis. Apart from rodents and humans, studies on sheep and bull have also identified lncRNAs potentially important for spermatogenesis. A number of these non-coding RNAs are strong candidates for further research on their roles in spermatogenesis.

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“…Secondly, the acroplaxome, a cytoskeletal plate composed of F-actin, keratin 5 modulates the exogenous forces generated by Sertoli cells. Moreover, the marginal ring of the acroplaxome and the perinuclear ring of the manchette reduce their diameter as they gradually descend along the nucleus toward the spermatid tail in a zipper-like movement that facilitates nuclear condensation and shaping [60,61]. Microtubules of manchette participate in intramanchette transport of proteins involved in spermatid development and removing residual cytoplasm [62].…”

Section: Discussionmentioning

confidence: 99%

Ethnic Differences in Spermatozoa Morphological Defects Diversity Among Men From Eastern Siberia, Russia

Клещев

Осадчук

Шантанова

et al. 2020

Preprint

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Background.Sperm morphology defects are considered to indicate several spermiogenesis anomalies, resulting in decreased men fertility, causing not only poor sperm morphology but also increased DNA fragmentation, chromatin condensation defects, increased oxidative stress. Consequently, detailed assessment of sperm morphological abnormalities in additional to counting percentage of sperm with normal morphology may be useful for andrologists and reproductive biologists. However, a little attention is paid to studying a regional and especially ethnic variability in the incidence of the sperm morphology defects. The aim of this research was to estimate ethnic variability in percentages of the sperm morphology abnormalities in men from general population living in the Siberian region of the Russian Federation.Methods.Male volunteers of Buryat (n=195), Yakut (n=130), and Slavic (n=89) ethnicity from the general population from Ulan-Ude and Yakutsk at least 5 years were enrolled in the study. Percentages of sperm morphological defects (according to the classification provided by the WHO laboratory manual, 2010) are counted after staining native ejaculate smears by Diff-Quick kits. Results.Among sperm defect, amorphous (62.25±12.18%), elongated (10.61±8.62%), pyriform(8.61±9.17%), and vacuolated heads (10.31±6.77%), abnormal acrosome (20.43±12.67%), asymmetrical insertion neck (18.33±6,43%), thick midpiece (6.21±3,41%), bent head (5.94±4,02%) and coiled tail (10.8±6.79%) were most frequent. In comparison with Buryats, Slavs and Yakuts were characterized by the decreased proportion sperm with normal morphology, the increased proportion of pyriform, elongated heads, abnormal acrosome, asymmertical tail insertion, excessive residual cytoplasm and increased teratozoospermia index (TZI). It was shown that impared progressive sperm motility and concentration associated with decreased percentage of sperm with normal morphology and increased proportion most of sperm morphology defects and TZI. However, increasing TZI in men with oligoastenozoospermia compared to men with astenozoospermia was revealed for Buryats only, suggesting ethnic features in the relationships between sperm morphology and concentration.Conclusion.The present study showed significant coordinated ethnic variability in the percentages of several sperm morphological defects in men from the general population located in Eastern Siberia. Damaged spermiogenesis processes underlying sperm head elongation and tail formation is considered to be significant contributors to geographical and ethnic variability of sperm morphology in men from the studied populations.

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Sperm Differentiation: The Role of Trafficking of Proteins

Cited by 35 publications

References 239 publications

Composition and function of the C1b/C1f region in the ciliary central apparatus

Composition and function of the C1b/C1f region in the ciliary central apparatus

Long non-coding RNAs (lncRNAs) in spermatogenesis and male infertility

Ethnic Differences in Spermatozoa Morphological Defects Diversity Among Men From Eastern Siberia, Russia

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